Human Mesothelin Alexa Fluor® 700-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects human Mesothelin in direct ELISAs.
  • Source
    Monoclonal Rat IgG2A Clone # 420411
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Mesothelin
    Accession # AAH09272
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 700
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB32652N, filled histogram) or isotype control antibody (Catalog # IC006N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

  • Entrez Gene IDs:
    10232 (Human); 56047 (Mouse); 60333 (Rat)
  • Alternate Names:
    CAK1 antigen; CAK1; megakaryocyte potentiating factor; mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Alexa Fluor® 700-conjugated Antibody

Ctrl IC006N
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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