Human Mesothelin PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB32652P
Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry.
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Product Details
Citations (9)
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Human Mesothelin PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Mesothelin in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 420411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below
Knockout Validated
Mesothelin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Mesothelin knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Mesothelin antibody in HeLa Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin PE‑conjugated Monoclonal Antibody (Catalog # FAB32652P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Knockout Validated Mesothelin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

Mesothelin Specificity is Shown by Flow Cytometry in Knockout Cell Line. Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin PE-conjugated Monoclonal Antibody (Catalog # FAB32652P, filled histogram) or isotype control antibody (Catalog # IC006P, open histogram). No staining in the Mesothelin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Entrez Gene IDs
10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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Citations for Human Mesothelin PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Targeting Immunosuppressive Tumor-Associated Macrophages Using Innate T Cells for Enhanced Antitumor Reactivity
    Authors: YR Li, J Brown, Y Yu, D Lee, K Zhou, ZS Dunn, R Hon, M Wilson, A Kramer, Y Zhu, Y Fang, L Yang
    Cancers, 2022;14(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. An Ex Vivo 3D Tumor Microenvironment-Mimicry Culture to Study TAM Modulation of Cancer Immunotherapy
    Authors: YR Li, Y Yu, A Kramer, R Hon, M Wilson, J Brown, L Yang
    Cells, 2022;11(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CRISPR/Cas9-mediated TGFbetaRII disruption enhances anti-tumor efficacy of human chimeric antigen receptor T cells in vitro
    Authors: K Alishah, M Birtel, E Masoumi, L Jafarzadeh, HR Mirzaee, J Hadjati, RH Voss, M Diken, S Asad
    Journal of Translational Medicine, 2021;19(1):482.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Clonal architecture in mesothelioma is prognostic and shapes the tumour microenvironment
    Authors: M Zhang, JL Luo, Q Sun, J Harber, AG Dawson, A Nakas, S Busacca, AJ Sharkey, D Waller, MT Sheaff, C Richards, P Wells-Jord, A Gaba, C Poile, EY Baitei, A Bzura, J Dzialo, M Jama, J Le Quesne, A Bajaj, L Martison, JA Shaw, C Pritchard, T Kamata, N Kuse, L Brannan, P De Philip, H Yang, G Griffiths, G Wilson, C Swanton, F Dudbridge, EJ Hollox, DA Fennell
    Nature Communications, 2021;12(1):1751.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Genetic and pharmacological targeting of A2a receptor improves function of anti-mesothelin CAR T cells
    Authors: E Masoumi, L Jafarzadeh, HR Mirzaei, K Alishah, K Fallah-Meh, H Rostamian, M Khakpoor-K, R Meshkani, F Noorbakhsh, J Hadjati
    J. Exp. Clin. Cancer Res., 2020;39(1):49.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Recombination of a dual-CAR-modified T lymphocyte to accurately eliminate pancreatic malignancy
    Authors: E Zhang, P Yang, J Gu, H Wu, X Chi, C Liu, Y Wang, J Xue, W Qi, Q Sun, S Zhang, J Hu, H Xu
    J Hematol Oncol, 2018;11(1):102.
    Species: Human
    Sample Types: Transfected Whole Cells
    Applications: Flow Cytometry
  7. High-throughput screening of rare metabolically active tumor cells in pleural effusion and peripheral blood of lung cancer patients
    Authors: Y Tang, Z Wang, Z Li, J Kim, Y Deng, Y Li, JR Heath, W Wei, S Lu, Q Shi
    Proc. Natl. Acad. Sci. U.S.A, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human CAR T cells with cell-intrinsic PD-1 checkpoint blockade resist tumor-mediated inhibition
    J Clin Invest, 2016;126(8):3130-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: CAR-T (Flow Cytometry)
  9. An in vivo platform for tumor biomarker assessment.
    Authors: Servais EL, Suzuki K, Colovos C
    PLoS ONE, 2011;6(10):e26722.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human Mesothelin PE-conjugated Antibody
By Anonymous on 12/23/2020
Application: Detection of Mesothelin on NCI-H226 lung squamous cell carcinoma cells. NCI-H226 cells were treated with the Human Mesothelin PE-conjugated Antibody (catalog # FAB32652P) (BLUE) or PE-conjugated Rat IgG2a isotype control (RED). Sample Tested: NCI-H226 lung squamous cell carcinoma cells Species: Human