Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Knockout Validated, ELISA, Flow Cytometry, CyTOF-ready

Cited:

Flow Cytometry, Immunocytochemistry, CAR-T (Flow Cytometry), Mass Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 420411
View all Mesothelin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272

Specificity

Detects human Mesothelin in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Mesothelin Antibody

Detection of Mesothelin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).
Mesothelin Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line

Mesothelin Specificity is Shown by Flow Cytometry in Knockout Cell Line

Mesothelin knockout HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram) followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B). No staining in the Mesothelin knockout HeLa cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Human Mesothelin Antibody in ELISA Standard Curve.

Human Mesothelin ELISA Standard Curve.

Recombinant Human Mesothelin protein was serially diluted 2-fold and captured by Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32652) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Applications for Human Mesothelin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human Mesothelin Monoclonal Antibody (Catalog # MAB32655).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Mesothelin DuoSet ELISA Kit (Catalog # DY3265) for convenient development of a sandwich ELISA or the Human Mesothelin Quantikine ELISA Kit (Catalog # DMSLN0) for a complete optimized ELISA.

Flow Cytometry

0.25 µg/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Knockout Validated

Mesothelin is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Mesothelin knockout HeLa cell line.

Reviewed Applications

Read 4 reviews rated 4.3 using MAB32652 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272

Additional Mesothelin Products

Product Documents for Human Mesothelin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Mesothelin Antibody

Customer Reviews for Human Mesothelin Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Showing  1 - 4 of 4 reviews Showing All
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  • Human Mesothelin Antibody
    Name: Pinar Aksoy
    Application: Flow Cytometry
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 12/08/2022
    Used the antibody at 1:100 dilution to stain HeLa cells. The secondary antibody was Rat F(ab)2 IgG (H+L) PE-conjugated antibody. Pink is the isotype control stained sample, and blue is the MSLN antibody stained sample. Iso control is Rat IgG2A.
    Human Mesothelin Antibody MAB32652
  • Human Mesothelin Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: NCI-H226 lung squamous cell carcinoma cells
    Species: Human
    Verified Customer | Posted 12/23/2020
    Detection of Mesothelin on NCI-H226 lung squamous cell carcinoma cells. NCI-H226 cells were treated with 67 nM of the Human Mesothelin Antibody (catalog # MAB32652) or rat IgG2a isotype control, followed by a secondary APC AffiniPure F(ab')₂ Fragment Goat Anti-Rat IgG, F(ab')₂ fragment specific. Isotype control (RED), human TROP2 antibody (Blue).
    Human Mesothelin Antibody MAB32652
  • Human Mesothelin Antibody
    Name: Jenna Nguyen
    Application: Affinity Purification
    Sample Tested: Ovarian cancer tissue
    Species: Mouse
    Verified Customer | Posted 07/27/2018
  • Human Mesothelin Isoform 1 Antibody
    Name: Patricia Culp
    Application: Flow Cytometry
    Sample Tested: AsPC1 human pancreatic cancer cell line
    Species: Human
    Verified Customer | Posted 02/22/2016
    Human Mesothelin Antibody MAB32652

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Protocols

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