Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 420411
View all Mesothelin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mesothelin
Glu296-Gly580
Accession # AAH09272

Specificity

Detects human Mesothelin in direct ELISAs.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human Mesothelin APC‑conjugated Antibody

Detection of Mesothelin antibody in HeLa Human Cell Line antibody by Flow Cytometry.

Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry.

HeLa human cervical epithelial carcinoma cell line was stained with Rat Anti-Human Mesothelin APC-conjugated Monoclonal Antibody (Catalog # FAB32652A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human Mesothelin APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HeLa human cervical epithelial carcinoma cell line

Reviewed Applications

Read 4 reviews rated 4 using FAB32652A in the following applications:

Spectra Viewer

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Mesothelin

Mesothelin is a 40 kDa glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes Megakaryocyte-Potentiating Factor (MPF). Mature human Mesothelin shares 60% amino acid identity with either mouse or rat Mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Alternate Names

CAK1, MPF, MSLN, SMR

Entrez Gene IDs

10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)

Gene Symbol

MSLN

UniProt

AAH09272

Additional Mesothelin Products

Product Documents for Human Mesothelin APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mesothelin APC‑conjugated Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Citations for Human Mesothelin APC‑conjugated Antibody

Customer Reviews for Human Mesothelin APC‑conjugated Antibody (4)

4 out of 5
4 Customer Ratings
5 Stars
50%
4 Stars
0%
3 Stars
50%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human Mesothelin APC-conjugated Antibody
    Name: Arun Kashyap
    Application: Flow Cytometry
    Sample Tested: Lung tissue
    Species: Human
    Verified Customer | Posted 06/22/2025
    Expression of human Mesothelin in single, live, CD45-negative cells in single cell suspension from a human lung cancer biopsy. Cells were treated with the human Mesothelin APC-conjugated antibody (BLUE) or Rat IgG2A APC isotype control antibody (RED)
    Human Mesothelin APC‑conjugated Antibody FAB32652A
  • Human Mesothelin APC-conjugated Antibody
    Name: William Jackson
    Application: Flow Cytometry
    Sample Tested: HeLa cells
    Species: Human
    Verified Customer | Posted 03/14/2024
    The antibody epitope is sensitive to enzymatic cleavage and this is not included in the spec sheet. Staining is completely prevented by Trypsin or Accutase.
    Human Mesothelin APC‑conjugated Antibody FAB32652A
  • Human Mesothelin APC-conjugated Antibody
    Name: Emanuele Sasso
    Application: Flow Cytometry
    Sample Tested: OVCAR-3 human ovarian carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/22/2019
    Human Mesothelin APC‑conjugated Antibody FAB32652A
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22498111
    Species: Human
    Verified Customer | Posted 01/23/2015

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