Human MICB APC-conjugated Antibody

(5 citations)   
  • Species Reactivity
  • Specificity
    Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
  • Source
    Monoclonal Mouse IgG2B Clone # 236511
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human MICB
    Accession # CAI18747
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB APC‑conjugated Monoclonal Antibody (Catalog # FAB1599A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

  • References:
    1. Groh, V. et al. (2001) Nature Immunol. 2:255.
    2. Stephens, H. (2001) Trends Immunol. 22:378.
    3. Bauer, S. et al. (1999) Science 285:727.
    4. Groh, V. et al. (2002) Nature 419:734.
    5. Steinle, A. et al. (2001) Immunogenetics 53:279.
    6. Pende, D. et al. (2002) Cancer Res. 62:6178.
    7. Salih, H. et al. (2003) Blood 102:1389.
  • Long Name:
    MHC Class I-related Protein B
  • Entrez Gene IDs:
    4277 (Human)
  • Alternate Names:
    MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog
Related Research Areas

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 5 of 5
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Sample Type
  1. Increase of IFN-gamma and TNF-alpha production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.
    Authors: Gomez-Lomeli P, Bravo-Cuellar A, Hernandez-Flores G, Jave-Suarez L, Aguilar-Lemarroy A, Lerma-Diaz J, Dominguez-Rodriguez J, Sanchez-Reyes K, Ortiz-Lazareno P
    Cancer Cell Int, 2014;14(1):100.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
    Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
    J Exp Med, 2013;210(12):2675-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012;86(16):8693-704.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines.
    Authors: Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K, Kiessling R
    J. Immunol., 2012;188(5):2136-45.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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