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Human MICB APC-conjugated Antibody

Catalog #: FAB1599A
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1599A-025
FAB1599A-100
Detection of MICB in K562 Human Cell Line by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human MICB APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Source
Monoclonal Mouse IgG2B Clone # 236511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICB
Ala23-Gly298
Accession # CAI18747
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB APC-conjugated Monoclonal Antibody (Catalog # FAB1599A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.
Long Name
MHC Class I-related Protein B
Entrez Gene IDs
4277 (Human)
Alternate Names
MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MICB; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog

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Citations for Human MICB APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Establishment of HLA class I and MICA/B null HEK-293T panel expressing single MICA alleles to detect anti-MICA antibodies
    Authors: JH Jeon, IC Baek, CH Hong, KH Park, H Lee, EJ Oh, TG Kim
    Scientific Reports, 2021;11(1):15716.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
    Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
    Cell Death & Disease, 2021;12(9):836.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Complete Remission with Reduction of High-risk Clones following Haploidentical NK Cell Therapy against MDS and AML
    Authors: AT Björklund, M Carlsten, E Sohlberg, LL Liu, T Clancy, M Karimi, S Cooley, JS Miller, M Klimkowska, M Schaffer, E Watz, KI Wikstrom, P Blomberg, BE Wahlin, M Palma, L Hansson, P Ljungman, E Hellström-, HG Ljunggren, KJ Malmberg
    Clin. Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. All-trans retinoic acid enhances cytotoxicity of CIK cells against human lung adenocarcinoma by upregulating MICA and IL-2 secretion
    Authors: XY Fan, PY Wang, C Zhang, YL Zhang, Y Fu, C Zhang, QX Li, JN Zhou, BE Shan, DW He
    Sci Rep, 2017;7(1):16481.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Low-dose bortezomib increases the expression of NKG2D and DNAM-1 ligands and enhances induced NK and ?? T cell-mediated lysis in multiple myeloma
    Authors: C Niu, H Jin, M Li, S Zhu, L Zhou, F Jin, Y Zhou, D Xu, J Xu, L Zhao, S Hao, W Li, J Cui
    Oncotarget, 2017;8(4):5954-5964.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Increase of IFN-gamma and TNF-alpha production in CD107a + NK-92 cells co-cultured with cervical cancer cell lines pre-treated with the HO-1 inhibitor.
    Authors: Gomez-Lomeli P, Bravo-Cuellar A, Hernandez-Flores G, Jave-Suarez L, Aguilar-Lemarroy A, Lerma-Diaz J, Dominguez-Rodriguez J, Sanchez-Reyes K, Ortiz-Lazareno P
    Cancer Cell Int, 2014;14(1):100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Modulation of NKG2D ligand expression and metastasis in tumors by spironolactone via RXRgamma activation.
    Authors: Leung W, Vong Q, Lin W, Janke L, Chen T, Leung W
    J Exp Med, 2013;210(12):2675-92.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Kaposi's sarcoma-associated herpesvirus ORF54/dUTPase downregulates a ligand for the NK activating receptor NKp44.
    Authors: Madrid, Alexis S, Ganem, Don
    J Virol, 2012;86(16):8693-704.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. HER2/HER3 signaling regulates NK cell-mediated cytotoxicity via MHC class I chain-related molecule A and B expression in human breast cancer cell lines.
    Authors: Okita R, Mougiakakos D, Ando T, Mao Y, Sarhan D, Wennerberg E, Seliger B, Lundqvist A, Mimura K, Kiessling R
    J. Immunol., 2012;188(5):2136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. Expression of the ULBP ligands for NKG2D by B-NHL cells plays an important role in determining their susceptibility to rituximab-induced ADCC.
    Authors: Inagaki A, Ishida T, Yano H, Ishii T, Kusumoto S, Ito A, Ri M, Mori F, Ding J, Komatsu H, Iida S, Ueda R
    Int. J. Cancer, 2009;125(1):212-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Isotype Controls

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Flow Cytometry Staining Buffer (1X)

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