Human MICB Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1599G-025
FAB1599G-100
Detection of MICB in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (4)
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Human MICB Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Source
Monoclonal Mouse IgG2B Clone # 236511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICB
Ala23-Gly298
Accession # CAI18747
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB1599G, filled histogram) or isotype control antibody (Catalog # IC0041G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.
Long Name
MHC Class I-related Protein B
Entrez Gene IDs
4277 (Human)
Alternate Names
MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MICB; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citations for Human MICB Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells
    Authors: Morimoto Y, Yamashita N, Daimon T et al.
    Journal for immunotherapy of cancer
  2. NKG2A and HLA-E define an alternative immune checkpoint axis in bladder cancer
    Authors: Bérengère Salomé, John P. Sfakianos, Daniel Ranti, Jorge Daza, Christine Bieber, Andrew Charap et al.
    Cancer Cell
  3. Targeting WEE1/AKT restores p53-dependent NK cell activation to induce immune checkpoint blockade responses in 'cold' melanoma
    Authors: SS Dinavahi, YC Chen, K Punnath, A Berg, M Herlyn, M Foroutan, ND Huntington, GP Robertson
    Cancer Immunology Research, 2022-06-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: FACS
  4. Overcoming Target Driven Fratricide for T Cell Therapy
    Authors: Eytan Breman, Benjamin Demoulin, Sophie Agaugué, Sebastien Mauën, Alexandre Michaux, Lorraine Springuel et al.
    Frontiers in Immunology

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