Human MICB Antibody

Catalog # Availability Size / Price Qty
MAB1599-SP
MAB1599-500
MAB1599-100
Detection of MICB in K562 Human Cell Line by Flow Cytometry.
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Product Details
Citations (36)
FAQs
Supplemental Products
Reviews (1)

Human MICB Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICB in direct ELISAs and Western blots. Does not cross-react with recombinant human MICA.
Source
Monoclonal Mouse IgG2B Clone # 236511
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICB
Ala23-Gly298
Accession # CAI18747
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human MICB Fc Chimera, aa 23-298 (Catalog # 1599-MB)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
MICB is specifically detected in K562 myelogenous leukemia parental cell line but is not detectable in MICB knockout K562 cell line.
 

Human MICB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human MICB Biotinylated Antibody (Catalog # BAF1599)

Standard: Recombinant Human MICB (aa 23-298) Fc Chimera Protein, CF (Catalog # 1599-MB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of MICB antibody in K562 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of MICB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

Knockout Validated MICB Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line. View Larger

MICB Specificity is Shown by Flow Cytometry in Knockout Cell Line. MICB knockout K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human MICB Monoclonal Antibody (Catalog # MAB1599, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B). No staining in the MICB knockout K562 cell line was observed. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MICB

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response (1-7).

References
  1. Groh, V. et al. (2001) Nature Immunol. 2:255.
  2. Stephens, H. (2001) Trends Immunol. 22:378.
  3. Bauer, S. et al. (1999) Science 285:727.
  4. Groh, V. et al. (2002) Nature 419:734.
  5. Steinle, A. et al. (2001) Immunogenetics 53:279.
  6. Pende, D. et al. (2002) Cancer Res. 62:6178.
  7. Salih, H. et al. (2003) Blood 102:1389.
Long Name
MHC Class I-related Protein B
Entrez Gene IDs
4277 (Human)
Alternate Names
MHC class I chain-related protein B; MHC class I mic-B antigen; MHC class I polypeptide-related sequence B; MICB; MIC-B; PERB11.2MHC class I-like molecule PERB11.2-IMX; stress inducible class I homolog

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Citations for Human MICB Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

36 Citations: Showing 1 - 10
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  1. CRISPR activation screen identifies BCL-2 proteins and B3GNT2 as drivers of cancer resistance to T cell-mediated cytotoxicity
    Authors: J Joung, PC Kirchgatte, A Singh, JH Cho, SP Nety, RC Larson, RK Macrae, R Deasy, YY Tseng, MV Maus, F Zhang
    Nature Communications, 2022;13(1):1606.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Immunomodulatory effect of NEDD8-activating enzyme inhibition in Multiple Myeloma: upregulation of NKG2D ligands and sensitization to Natural Killer cell recognition
    Authors: S Petillo, C Capuano, R Molfetta, C Fionda, A Mekhloufi, C Pighi, F Antonangel, A Zingoni, A Soriani, MT Petrucci, R Galandrini, R Paolini, A Santoni, M Cippitelli
    Cell Death & Disease, 2021;12(9):836.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. A slowly cleaved viral signal peptide acts as a protein-integral immune evasion domain
    Authors: E Seidel, L Dassa, S Kahlon, B Tirosh, A Halenius, T Seidel Mal, O Mandelboim
    Nature Communications, 2021;12(1):2061.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The human cytomegalovirus protein UL147A downregulates the most prevalent MICA allele: MICA*008, to evade NK cell-mediated killing
    Authors: E Seidel, L Dassa, C Schuler, E Oiknine-Dj, DG Wolf, VTK Le-Trillin, O Mandelboim
    PloS Pathogens, 2021;17(5):e1008807.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Rac1/ROCK-driven membrane dynamics promote natural killer cell cytotoxicity via granzyme-induced necroptosis
    Authors: Y Zhu, J Xie, J Shi
    Bmc Biology, 2021;19(1):140.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Human Metapneumovirus Escapes NK Cell Recognition through the Downregulation of Stress-Induced Ligands for NKG2D
    Authors: M Diab, D Schmiedel, E Seidel, E Bacharach, O Mandelboim
    Viruses, 2020;12(7):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Allogeneic ADSCs Induce the Production of Alloreactive Memory-CD8 T Cells through HLA-ABC Antigens
    Authors: SH Chang, HJ Kim, CG Park
    Cells, 2020;9(5):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Targetable mechanisms driving immunoevasion of persistent senescent cells link chemotherapy-resistant cancer to aging
    Authors: DP Muñoz, SM Yannone, A Daemen, Y Sun, F Vakar-Lope, M Kawahara, AM Freund, F Rodier, JD Wu, PY Desprez, DH Raulet, PS Nelson, LJ van 't Vee, J Campisi, JP Coppé
    JCI Insight, 2019;5(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  9. NK cell detachment from target cells is regulated by successful cytotoxicity and influences cytokine production
    Authors: M Anft, P Netter, D Urlaub, I Prager, S Schaffner, C Watzl
    Cell. Mol. Immunol., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. The combination of ionizing radiation and proteasomal inhibition by bortezomib enhances the expression of NKG2D ligands in multiple myeloma cells
    Authors: YS Lee, W Heo, J Nam, YH Jeung, J Bae
    J. Radiat. Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Vigilin Regulates the Expression of the Stress-Induced Ligand MICB by Interacting with Its 5' Untranslated Region
    Authors: O Berhani, D Nachmani, R Yamin, D Schmiedel, Y Bar-On, O Mandelboim
    J. Immunol., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Increasing TIMP3 expression by hypomethylating agents diminishes soluble MICA, MICB and ULBP2 shedding in acute myeloid leukemia, facilitating NK cell-mediated immune recognition
    Authors: AB Raneros, AM Puras, RM Rodriguez, E Colado, T Bernal, E Anguita, AV Mogorron, AC Gil, JR Vidal-Cast, L Márquez-Ki, PD Bulnes, AM Marin, MCG Garay, B Suarez-Alv, C Lopez-Larr
    Oncotarget, 2017;8(19):31959-31976.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Purine nucleotide metabolism regulates expression of the human immune ligand MICA
    Authors: MT McCarthy, G Moncayo, TK Hiron, NA Jakobsen, A Valli, T Soga, J Adam, CA O'Callagha
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. MicroRNA-519a-3p mediates apoptosis resistance in breast cancer cells and their escape from recognition by natural killer cells
    Authors: C Breunig, J Pahl, M Küblbeck, M Miller, D Antonelli, N Erdem, C Wirth, R Will, A Bott, A Cerwenka, S Wiemann
    Cell Death Dis, 2017;8(8):e2973.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Human cytomegalovirus escapes immune recognition by NK cells through the downregulation of B7-H6 by the viral genes US18 and US20
    Authors: Y Charpak-Am, T Kubsch, E Seidel, E Oiknine-Dj, N Cavaletto, R Yamin, D Schmiedel, D Wolf, G Gribaudo, M Messerle, L Cicin-Sain, O Mandelboim
    Sci Rep, 2017;7(1):8661.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Combination treatment with decitabine and ionizing radiation enhances tumor cells susceptibility of T cells
    Sci Rep, 2016;6(0):32470.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Genotoxic Stress Induces Senescence-Associated ADAM10-Dependent Release of NKG2D MIC Ligands in Multiple Myeloma Cells.
    Authors: Zingoni A, Cecere F, Vulpis E, Fionda C, Molfetta R, Soriani A, Petrucci M, Ricciardi M, Fuerst D, Amendola M, Mytilineos J, Cerboni C, Paolini R, Cippitelli M, Santoni A
    J Immunol, 2015;195(2):736-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  18. Cytotoxicity and infiltration of human NK cells in in vivo-like tumor spheroids.
    Authors: Giannattasio A, Weil S, Kloess S, Ansari N, Stelzer E, Cerwenka A, Steinle A, Koehl U, Koch J
    BMC Cancer, 2015;15(0):351.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. NKG2D-dependent activation of dendritic epidermal T cells in contact hypersensitivity.
    Authors: Nielsen M, Dyring-Andersen B, Schmidt J, Witherden D, Lovato P, Woetmann A, Odum N, Poulsen S, Havran W, Geisler C, Bonefeld C
    J Invest Dermatol, 2015;135(5):1311-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: FACS
  20. MicroRNA editing facilitates immune elimination of HCMV infected cells.
    Authors: Nachmani D, Zimmermann A, Oiknine Djian E, Weisblum Y, Livneh Y, Khanh Le V, Galun E, Horejsi V, Isakov O, Shomron N, Wolf D, Hengel H, Mandelboim O
    PLoS Pathog, 2014;10(2):e1003963.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Two novel human cytomegalovirus NK cell evasion functions target MICA for lysosomal degradation.
    Authors: Fielding C, Aicheler R, Stanton R, Wang E, Han S, Seirafian S, Davies J, McSharry B, Weekes M, Antrobus P, Prod'homme V, Blanchet F, Sugrue D, Cuff S, Roberts D, Davison A, Lehner P, Wilkinson G, Tomasec P
    PLoS Pathog, 2014;10(5):e1004058.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  22. Altered microRNA expression after infection with human cytomegalovirus leads to TIMP3 downregulation and increased shedding of metalloprotease substrates, including MICA.
    Authors: Esteso G, Luzon E, Sarmiento E, Gomez-Caro R, Steinle A, Murphy G, Carbone J, Vales-Gomez M, Reyburn H
    J Immunol, 2014;193(3):1344-52.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  23. RNA-binding proteins regulate the expression of the immune activating ligand MICB.
    Authors: Nachmani, Daphna, Gutschner, Tony, Reches, Adi, Diederichs, Sven, Mandelboim, Ofer
    Nat Commun, 2014;5(0):4186.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Cytotoxicity of CD56(bright) NK cells towards autologous activated CD4+ T cells is mediated through NKG2D, LFA-1 and TRAIL and dampened via CD94/NKG2A.
    Authors: Nielsen N, Odum N, Urso B, Lanier LL, Spee P
    PLoS ONE, 2012;7(2):e31959.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. MiR-10b Downregulates the Stress-Induced Cell Surface Molecule MICB, a Critical Ligand for Cancer Cell Recognition by Natural Killer Cells.
    Authors: Tsukerman P, Stern-Ginossar N, Gur C, Glasner A, Nachmani D, Bauman Y, Yamin R, Vitenshtein A, Stanietsky N, Bar-Mag T, Lankry D, Mandelboim O
    Cancer Res, 2012;72(21):5463-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Thermal- and Oxidative Stress Causes Enhanced Release of NKG2D Ligand-Bearing Immunosuppressive Exosomes in Leukemia/Lymphoma T and B Cells.
    Authors: Hedlund M, Nagaeva O, Kargl D, Baranov V, Mincheva-Nilsson L
    PLoS ONE, 2011;6(2):e16899.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  27. Rapid expansion and long-term persistence of elevated NK cell numbers in humans infected with hantavirus.
    Authors: Bjorkstrom NK, Lindgren T, Stoltz M, Fauriat C, Braun M, Evander M, Michaelsson J, Malmberg KJ, Klingstrom J, Ahlm C, Ljunggren HG
    J. Exp. Med., 2011;208(1):13-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. DNAM-1 ligand expression on Ag-stimulated T lymphocytes is mediated by ROS-dependent activation of DNA-damage response: relevance for NK-T cell interaction.
    Authors: Ardolino M, Zingoni A, Cerboni C, Cecere F, Soriani A, Iannitto ML, Santoni A
    Blood, 2011;117(18):4778-86.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  29. Post-translational modification of the NKG2D ligand RAET1G leads to cell surface expression of a glycosylphosphatidylinositol-linked isoform.
    Authors: Ohashi M, Eagle RA, Trowsdale J
    J. Biol. Chem., 2010;285(22):16408-15.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  30. Inhibition of NKG2D expression in NK cells by cytokines secreted in response to human cytomegalovirus infection.
    Authors: Muntasell A, Magri G, Pende D, Angulo A, Lopez-Botet M
    Blood, 2010;115(25):5170-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. Obstructing shedding of the immunostimulatory MHC class I chain-related gene B prevents tumor formation.
    Authors: Wu JD, Atteridge CL, Wang X, Seya T, Plymate SR
    Clin. Cancer Res., 2009;15(2):632-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Cutting edge: the metalloproteinase ADAM17/TNF-alpha-converting enzyme regulates proteolytic shedding of the MHC class I-related chain B protein.
    Authors: Boutet P, Aguera-Gonzalez S, Atkinson S, Pennington CJ, Edwards DR, Murphy G, Reyburn HT, Vales-Gomez M
    J. Immunol., 2009;182(1):49-53.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  33. Detuning CD8+ T lymphocytes by down-regulation of the activating receptor NKG2D: role of NKG2D ligands released by activated T cells.
    Authors: Cerboni C, Ardolino M, Santoni A, Zingoni A
    Blood, 2009;113(13):2955-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  34. NK cells recognize and lyse Ewing sarcoma cells through NKG2D and DNAM-1 receptor dependent pathways.
    Authors: Verhoeven DH, de Hooge AS, Mooiman EC, Santos SJ, ten Dam MM, Gelderblom H, Melief CJ, Hogendoorn PC, Egeler RM, van Tol MJ, Schilham MW, Lankester AC
    Mol. Immunol., 2008;45(15):3917-25.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. Induction of MHC class I-related chain B (MICB) by 5-aza-2'-deoxycytidine.
    Authors: Tang KF, He CX, Zeng GL, Wu J, Song GB, Shi YS, Zhang WG, Huang AL, Steinle A, Ren H
    Biochem. Biophys. Res. Commun., 2008;370(4):578-83.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  36. Cancer cells become susceptible to natural killer cell killing after exposure to histone deacetylase inhibitors due to glycogen synthase kinase-3-dependent expression of MHC class I-related chain A and B.
    Authors: Skov S, Pedersen MT, Andresen L, Straten PT, Woetmann A, Odum N
    Cancer Res., 2005;65(23):11136-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human MICB Antibody
By Anonymous on 10/24/2022
Application: Flow Sample Tested: T98G human glioblastoma cell line Species: Human

This antibody, followed by anti-mouse secondary antibodies, achieves a clean staining of surface MICB in glioma cells