Human MICL/CLEC12A PE-conjugated Antibody

Clone 687317 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB2946P
Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry.
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Human MICL/CLEC12A PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human MICL/CLEC12A in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) CLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A, rhCD302/CLEC13A recombinant mouse (rm) CLEC4B2, or rmMICL is observed.
Source
Monoclonal Mouse IgG2B Clone # 687317
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A
Thr67-Ala265
Accession # Q5QGZ9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry. View Larger

Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human MICL/CLEC12A PE-conjugated Monoclonal Antibody (Catalog # FAB2946P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MICL/CLEC12A

Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.

Long Name
Myeloid Inhibitory C-type Lectin-like Receptor
Entrez Gene IDs
160364 (Human); 232413 (Mouse)
Alternate Names
CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL

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Human MICL/CLEC12A PE-conjugated Antibody
By Jenna Nguyen on 05/07/2019
Application: ICC/IF Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line Species: Mouse