Detects human MICL/CLEC12A in direct ELISAs.
In direct ELISAs, no cross-reactivity
with recombinant human (rh) CLEC1, 2, 2A, 3B, 9A, 10A, 12B, 14A,
rhCD302/CLEC13A recombinant mouse (rm) CLEC4B2, or rmMICL is observed.
Monoclonal Mouse IgG2B Clone # 687317
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human MICL/CLEC12A Thr67-Ala265 Accession # Q5QGZ9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of MICL/CLEC12A in Human Peripheral Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human MICL/CLEC12A Monoclonal Antibody (Catalog # MAB2946, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human MICL (myeloid inhibitory C-type lectin-like receptor; also CLL-1 and KLRL-1) is a 75 kDa member of the group V C-type lectin-like receptor family of molecules. Group V molecules have a lectin-type domain that binds non-sugar ligands. MICL is a 265 amino acid (aa) type II transmembrane (TM) glycoprotein that contains a 200 aa extracellular domain (ECD). Multiple isoforms of MICL are reported. One potentially utilizes an alternate start site that adds 10 aa to the cytoplasmic domain. There is one potential soluble form that lacks the TM segment, and two truncated ECD isoforms. Human MICL ECD is 53%, 65%, and 55% aa identical to the ECD in mouse, dog and cow, respectively.
Myeloid Inhibitory C-type Lectin-like Receptor
Entrez Gene IDs:
160364 (Human); 232413 (Mouse)
CD371; CLEC12A; CLL1; CLL-1; C-type lectin domain family 12, member A; DCAL-2; MICL
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.