MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.
Loading...
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), Platelet-poor EDTA Plasma (10 µL), Platelet-poor Heparin Plasma (10 µL), Urine (10 µL), Human Milk (10 µL)
Sensitivity
0.068 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Loading...
Product Summary for Human MIF Quantikine ELISA Kit
The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Natural and recombinant human MIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 1.16 | 3.56 | 7.27 | 1.03 | 3.20 | 6.46 |
| Standard Deviation | 0.07 | 0.18 | 0.33 | 0.10 | 0.27 | 0.61 |
| CV% | 6.0 | 5.1 | 4.5 | 9.7 | 8.4 | 9.4 |
Recovery for Human MIF Quantikine ELISA Kit
The recovery of MIF spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-104 |
| Platelet-poor EDTA Plasma (n=5) | 95 | 80-108 |
| Platelet-poor Heparin Plasma (n=5) | 96 | 86-108 |
| Serum (n=4) | 99 | 94-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Scientific Data Images for Human MIF Quantikine ELISA Kit
Human MIF ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MIF
Long Name
Macrophage Migration Inhibitory Factor
Alternate Names
EC 5.3.2.1, EC 5.3.3.12, GIFmacrophage migration inhibitory factor, GLIF, Glycosylation-inhibiting factor, L-dopachrome isomerase, L-dopachrome tautomerase, macrophage migration inhibitory factor (glycosylation-inhibiting factor), MMIF, Phenylpyruvate tautomerase
Gene Symbol
MIF
Additional MIF Products
Product Documents for Human MIF Quantikine ELISA Kit
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human MIF Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Related Research Areas
Citations for Human MIF Quantikine ELISA Kit
Powered by Bioz
Customer Reviews for Human MIF Quantikine ELISA Kit (2)
5 out of 5
2 Customer Ratings
Have you used Human MIF Quantikine ELISA Kit?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Customer Images
Showing
1
-
2 of
2 reviews
Showing All
Filter By:
-
Sample Tested: 293T human embryonic kidney cell lineVerified Customer | Posted 06/18/2022
-
Sample Tested: EDTA PlasmaVerified Customer | Posted 05/26/2020
There are no reviews that match your criteria.
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars