hh Human MIF Quantikine ELISA Kit DMF00B: R&D Systems

Human MIF Quantikine ELISA Kit

(18 citations)   
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL), Human Milk (10 uL)
  • Sensitivity
    0.068 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
  • Specificity
    Natural and recombinant human MIF
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC122, Quantikine Immunoassay Control Set 694 for Human MIF - Please Inquire
Product Summary
The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1.163.567.271.033.26.46
Standard Deviation0.070.180.330.10.270.61
CV%65.14.59.78.49.4

Recovery

The recovery of MIF spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 86-104
Platelet-poor EDTA Plasma (n=5) 95 80-108
Platelet-poor Heparin Plasma (n=5) 96 86-108
Serum (n=4) 99 94-104
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human MIF Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MIF
MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.
    • Long Name
      Macrophage Migration Inhibitory Factor
    • Entrez Gene IDs
      4282 (Human); 17319 (Mouse);
    • Alternate Names
      EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    18 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. MIF and D-DT are potential disease severity modifiers in male MS subjects
      Authors: G Benedek, R Meza-Romer, K Jordan, Y Zhang, H Nguyen, G Kent, J Li, E Siu, J Frazer, M Piecychna, X Du, A Sreih, L Leng, J Wiedrick, SJ Caillier, H Offner, JR Oksenberg, V Yadav, D Bourdette, R Bucala, AA Vandenbark
      Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
      Species: Human
      Sample Type: Plasma
    2. Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane
      Authors: R Kumar, T de Mooij, TE Peterson, T Kaptzan, AJ Johnson, DJ Daniels, IF Parney
      PLoS ONE, 2017;12(6):e0179012.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
      Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
      J Neuroinflammation, 2017;14(1):126.
      Species: Human
      Sample Type: Serum
    4. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
      Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
      J Transl Med, 2017;15(1):121.
      Species: Human
      Sample Type: Plasma
    5. Comparison of macrophage migration inhibitory factor and neutrophil gelatinase-associated lipocalin-2 to predict acute kidney injury after liver transplantation: An observational pilot study
      Authors: J Baron-Stef, J Schiefer, EJ Miller, GA Berlakovic, DM Baron, P Faybik
      PLoS ONE, 2017;12(8):e0183162.
      Species: Human
      Sample Type: Serum
    6. Significance of the vascular endothelial growth factor and the macrophage migration inhibitory factor in the progression of hepatocellular carcinoma.
      Authors: Wang D, Luo L, Chen W, Chen L, Zeng W, Li W, Huang X
      Oncol Rep, 2014;31(3):1199-204.
      Species: Human
      Sample Type: Serum
    7. Effects of high intensity training and high volume training on endothelial microparticles and angiogenic growth factors.
      Authors: Wahl P, Jansen F, Achtzehn S, Schmitz T, Bloch W, Mester J, Werner N
      PLoS ONE, 2014;9(4):e96024.
      Species: Human
      Sample Type: Serum
    8. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
      Authors: Kreutz M, Karrer S, Hoffmann P
      J. Invest. Dermatol., 2012;132(1):179-87.
      Species: Human
      Sample Type: Serum
    9. Serum macrophage migration-inhibitory factor as a diagnostic and prognostic biomarker for gastric cancer.
      Authors: Xia HH, Yang Y, Chu KM, Gu Q, Zhang YY, He H, Wong WM, Leung SY, Yuen ST, Yuen MF, Chan AO, Wong BC
      Cancer, 2009;115(23):5441-9.
      Species: Human
      Sample Type: Serum
    10. Identification of macrophage migration inhibitory factor and human neutrophil peptides 1-3 as potential biomarkers for gastric cancer.
      Authors: Mohri Y, Mohri T, Wei W, Qi YJ, Martin A, Miki C, Kusunoki M, Ward DG, Johnson PJ
      Br. J. Cancer, 2009;101(2):295-302.
      Species: Human
      Sample Type: Serum
    11. Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
      Authors: Wang B, Wood IS, Trayhurn P
      J. Endocrinol., 2008;198(1):127-34.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. The role of macrophage migration inhibitory factor in the cascade of events leading to reperfusion-induced inflammatory injury and lethality.
      Authors: Amaral FA, Fagundes CT, Guabiraba R, Vieira AT, Souza AL, Russo RC, Soares MP, Teixeira MM, Souza DG
      Am. J. Pathol., 2007;171(6):1887-93.
      Species: Mouse
      Sample Type: Serum
    13. UVA induces granzyme B in human keratinocytes through MIF: implication in extracellular matrix remodeling.
      Authors: Hernandez-Pigeon H, Jean C, Charruyer A, Haure MJ, Baudouin C, Charveron M, Quillet-Mary A, Laurent G
      J. Biol. Chem., 2007;282(11):8157-64.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Panel of markers can accurately predict endometriosis in a subset of patients.
      Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
      Fertil. Steril., 2007;89(5):1073-81.
      Species: Human
      Sample Type: Serum
    15. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
      Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
      Blood, 2007;111(4):2339-46.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
      Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
      Histopathology, 2005;46(6):659-67.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
      Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
      Endocrinology, 2004;145(9):4084-93.
      Species: Rat
      Sample Type: Tissue Homogenates
    18. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
      Authors: Schubert T, Horch RE
      Ann. Surg., 2004;240(2):340-9.
      Species: Rat
      Sample Type: Tissue Homogenates
    Expand to show all 18 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Set 694 for Human MIF

    Ctrl QC122
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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