Human MIF Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
SMF00B
DMF00B
PDMF00B
Control Products Available
Human MIF Standard Curve
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Product Details
Citations (20)
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Human MIF Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Platelet-poor EDTA Plasma (10 uL), Platelet-poor Heparin Plasma (10 uL), Urine (10 uL), Human Milk (10 uL)
Sensitivity
0.068 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk)
Specificity
Natural and recombinant human MIF
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human MIF Immunoassay is a 4.5 hour solid phase ELISA designed to measure human MIF in cell culture supernates, serum, plasma, urine, and human milk. It contains E. coli-expressed recombinant human MIF and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human MIF showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human MIF.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Urine, Human Milk

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.16 3.56 7.27 1.03 3.2 6.46
Standard Deviation 0.07 0.18 0.33 0.1 0.27 0.61
CV% 6 5.1 4.5 9.7 8.4 9.4

Recovery

The recovery of MIF spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 95 86-104
Platelet-poor EDTA Plasma (n=5) 95 80-108
Platelet-poor Heparin Plasma (n=5) 96 86-108
Serum (n=4) 99 94-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIF were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line
Human MIF ELISA Linearity

Data Examples

Human MIF Standard Curve

Product Datasheets

Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: MIF

MIF (macrophage migration inhibitory factor) is a pro-inflammatory cytokine that is released during microbial infection, ARDS, rheumatoid arthritis, atherosclerosis, type 2 diabetes, and cancer. It signals through CXCR4, CXCR7, or complexes of CD74-CD44. MIF exhibits potent paracrine and autocrine stimulatory effects on cell growth and survival and enhances the production of inflammatory cytokines. It also inhibits the effectiveness of immunosuppressive glucocorticoids.

Long Name:
Macrophage Migration Inhibitory Factor
Entrez Gene IDs:
4282 (Human); 17319 (Mouse)
Alternate Names:
EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human MIF Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. Comparison of macrophage migration inhibitory factor and neutrophil gelatinase-associated lipocalin-2 to predict acute kidney injury after liver transplantation: An observational pilot study
    Authors: J Baron-Stef, J Schiefer, EJ Miller, GA Berlakovic, DM Baron, P Faybik
    PLoS ONE, 2017;12(8):e0183162.
    Species: Human
    Sample Types: Serum
  2. Graft-derived macrophage migration inhibitory factor correlates with hepatocellular injury in patients undergoing liver transplantation
    Authors: J Baron-Stef, J Schiefer, EJ Miller, W Plöchl, CG Krenn, GA Berlakovic, DM Baron, P Faybik
    Clin Transplant, 2017;0(0):.
    Species: Human
    Sample Types: Complex Sample Type
  3. Temporal trend of autonomic nerve function and HSP27, MIF and PAI-1 in type 1 diabetes
    Authors: M Zimmerman, S Rolandsson, H Skärstrand, K Pourhamidi, A Gottsäter, P Wollmer, O Rolandsson, G Westergren, LB Dahlin
    J Clin Transl Endocrinol, 2017;8(0):15-21.
    Species: Human
    Sample Types: Serum
  4. Modulating glioma-mediated myeloid-derived suppressor cell development with sulforaphane
    Authors: R Kumar, T de Mooij, TE Peterson, T Kaptzan, AJ Johnson, DJ Daniels, IF Parney
    PLoS ONE, 2017;12(6):e0179012.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017;15(1):121.
    Species: Human
    Sample Types: Plasma
  6. MIF and D-DT are potential disease severity modifiers in male MS subjects
    Authors: G Benedek, R Meza-Romer, K Jordan, Y Zhang, H Nguyen, G Kent, J Li, E Siu, J Frazer, M Piecychna, X Du, A Sreih, L Leng, J Wiedrick, SJ Caillier, H Offner, JR Oksenberg, V Yadav, D Bourdette, R Bucala, AA Vandenbark
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Human
    Sample Types: Plasma
  7. The increased concentration of macrophage migration inhibitory factor in serum and cerebrospinal fluid of patients with tick-borne encephalitis
    Authors: S Grygorczuk, M Parczewski, R ?wierzbi?s, P Czupryna, A Moniuszko, J Dunaj, M Kondrusik, S Pancewicz
    J Neuroinflammation, 2017;14(1):126.
    Species: Human
    Sample Types: Serum
  8. Significance of the vascular endothelial growth factor and the macrophage migration inhibitory factor in the progression of hepatocellular carcinoma.
    Authors: Wang D, Luo L, Chen W, Chen L, Zeng W, Li W, Huang X
    Oncol Rep, 2014;31(3):1199-204.
    Species: Human
    Sample Types: Serum
  9. Effects of high intensity training and high volume training on endothelial microparticles and angiogenic growth factors.
    Authors: Wahl P, Jansen F, Achtzehn S, Schmitz T, Bloch W, Mester J, Werner N
    PLoS ONE, 2014;9(4):e96024.
    Species: Human
    Sample Types: Serum
  10. Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
    Authors: Kreutz M, Karrer S, Hoffmann P
    J. Invest. Dermatol., 2012;132(1):179-87.
    Species: Human
    Sample Types: Serum
  11. Serum macrophage migration-inhibitory factor as a diagnostic and prognostic biomarker for gastric cancer.
    Authors: Xia HH, Yang Y, Chu KM, Gu Q, Zhang YY, He H, Wong WM, Leung SY, Yuen ST, Yuen MF, Chan AO, Wong BC
    Cancer, 2009;115(23):5441-9.
    Species: Human
    Sample Types: Serum
  12. Identification of macrophage migration inhibitory factor and human neutrophil peptides 1-3 as potential biomarkers for gastric cancer.
    Authors: Mohri Y, Mohri T, Wei W, Qi YJ, Martin A, Miki C, Kusunoki M, Ward DG, Johnson PJ
    Br. J. Cancer, 2009;101(2):295-302.
    Species: Human
    Sample Types: Serum
  13. Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes.
    Authors: Wang B, Wood IS, Trayhurn P
    J. Endocrinol., 2008;198(1):127-34.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Panel of markers can accurately predict endometriosis in a subset of patients.
    Authors: Seeber B, Sammel MD, Fan X, Gerton GL, Shaunik A, Chittams J, Barnhart KT
    Fertil. Steril., 2007;89(5):1073-81.
    Species: Human
    Sample Types: Serum
  15. The role of macrophage migration inhibitory factor in the cascade of events leading to reperfusion-induced inflammatory injury and lethality.
    Authors: Amaral FA, Fagundes CT, Guabiraba R, Vieira AT, Souza AL, Russo RC, Soares MP, Teixeira MM, Souza DG
    Am. J. Pathol., 2007;171(6):1887-93.
    Species: Mouse
    Sample Types: Serum
  16. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  17. Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis.
    Authors: Ren Y, Lin CL, Li Z, Chen XY, Huang X, Lui V, Nicholls J, Lan HY, Tam PK
    Histopathology, 2005;46(6):659-67.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Insulin treatment improves hepatic morphology and function through modulation of hepatic signals after severe trauma.
    Authors: Schubert T, Horch RE
    Ann. Surg., 2004;240(2):340-9.
    Species: Rat
    Sample Types: Tissue Homogenates
  19. Insulin attenuates the systemic inflammatory response in endotoxemic rats.
    Authors: Jeschke MG, Klein D, Bolder U, Einspanier R
    Endocrinology, 2004;145(9):4084-93.
    Species: Rat
    Sample Types: Tissue Homogenates
  20. MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
    Authors: Rolandsson Enes S, Andersson Sjoland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
    Sci Rep, 0;6(0):29160.
    Species: Human
    Sample Types: Cell Culture Supernates

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