Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Mimecan Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Mimecan
Ala20-Phe298
Accession # P20774

Specificity

Detects human Mimecan in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse Mimecan is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Mimecan Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Mimecan

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mimecan

Mimecan, also known as KSPG25 (25 kDa Keratan Sulfate Proteoglycan) and Osteoglycin, is an extracellular matrix protein belonging to the class III subfamily of the small leucine rich proteoglycan (SLRP) family. SLRPs are characterized by leucine‑rich repeats in the central domain, which is flanked by N- and C‑terminal cysteine‑rich domains. The core proteins contain N-linked oligosaccharides as well as glycosaminoglycan side chains. Mimecan is synthesized as a preproprotein that is processed to generate proteins of different sizes including Osteoglycin (containing 93 most C‑terminal amino acid residues) and Mimecan (containing 223 most C‑terminal residues). Human Mimecan shares approximately 85% amino acid sequence homology with the mouse or rat Mimecan.

Alternate Names

KSPG25, OGN, OIF, SLRR3A

Entrez Gene IDs

4969 (Human); 18295 (Mouse)

Gene Symbol

OGN

UniProt

P20774

Additional Mimecan Products

Product Documents for Human Mimecan Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Mimecan Biotinylated Antibody

For research use only

Related Research Areas

Proteoglycans

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