Human MIS RII DuoSet ELISA

Key Product Details

Product Specifications

Scientific Data Images for Human MIS RII DuoSet ELISA

Kit Contents for Human MIS RII DuoSet ELISA

• Capture Antibody
• Detection Antibody
• Recombinant Standard
• Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Background: MIS RII

Müllerian inhibiting substance receptor II (MIS RII) is a type II TGF-beta superfamily receptor that forms a heteromeric complex with various type I receptors to mediate MIS signaling. Mature MIS RII has a cysteine-rich, glycosylated, extracellular domain, and a cytoplasmic region containing a serine/threonine kinase domain. Rat MIS RII shares 95% and 82% aa sequence identity with its mouse and human orthologs, respectively. Embryonically, it is expressed in mesenchymal cells surrounding the Müllerian ducts. Postnatally, it is expressed in uterine tissues and Leydig cells, showing coexpression with MIS in the testicular Sertoli and ovarian granulosa cells.

Additional MIS RII Products

• All Products for MIS RII
• MIS RII cDNA Clones
• MIS RII Lysates
• MIS RII Primary Antibodies
• MIS RII Proteins and Enzymes

Product Documents for Human MIS RII DuoSet ELISA