Stop Solution 2N Sulfuric Acid Summary
INTENDED USE & DESCRIPTION
Stop Solution (Part # 895032) contains 15 vials (6 mL/vial) of 2N sulfuric acid for the stopping the action of horseradish peroxidase on the tetramethylbenzidine substrate in an ELISA.
STORAGE & STABILITY
Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.
Add 50-100 µL/well to stop the action of horseradish peroxidase on the substrate, tetramethylbenzidine. If the color in the wells is green or the color change does not appear to be uniform, gently tap the plate to ensure thorough mixing.
TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE
- Caution: Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use.
Preparation and Storage
Citations for Stop Solution 2N Sulfuric Acid
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
Sci Rep, 2017;7(1):11326. 2017
Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
EBioMedicine, 2017;0(0):. 2017
IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
JCI Insight, 2017;2(10):. 2017
Proprotein convertase subtilisin/Kexin type 9 (PCSK9) single domain antibodies are potent inhibitors of LDL receptor degradation
Authors: Elodie Weider
J Biol Chem, 2016;0(0):. 2016
Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
PLoS ONE, 2015;10(1):e0116772. 2015
Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum.
Authors: Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S
PLoS ONE, 2013;8(3):e56186. 2013
The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
PLoS Pathog, 2013;9(12):e1003852. 2013
First-trimester follistatin-like-3 levels in pregnancies complicated by subsequent gestational diabetes mellitus.
Authors: Thadhani R, Powe CE, Tjoa ML
Diabetes Care, 2010;33(3):664-9. 2010
Soluble erythropoietin receptor contributes to erythropoietin resistance in end-stage renal disease.
Authors: Khankin EV, Mutter WP, Tamez H
PLoS ONE, 2010;5(2):e9246. 2010
Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
Authors: Palmer JC, Baig S, Kehoe PG, Love S
Am. J. Pathol., 2009;175(1):262-70. 2009
Nanobacteria are mineralo fetuin complexes.
Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
PLoS Pathog., 2008;4(2):e41. 2008
Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
Clin. Exp. Immunol., 2008;152(2):328-35. 2008
Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
Chest, 2007;132(1):58-63. 2007
Leptin Levels Are Higher in Whole Compared to Skim Human Milk, Supporting a Cellular Contribution.
Authors: Kugananthan S, Lai C, Gridneva Z, Mark P, Geddes D, Kakulas F
Nutrients, 0;8(11):. 0
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Reviews for Stop Solution 2N Sulfuric Acid
Average Rating: 4.8 (Based on 13 Reviews)
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Used stop solution to read the plate and it works perfect
Perfect for stopping the action of HRP on TMB in ELISA