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Substrate Reagent Pack

Catalog #: DY999
114 Citations 47 Reviews Datasheet / COA / SDS
Newer Version Available: DY999B
NEW
8 vials Color A, 8 vials Color B
Catalog # Availability Size / Price Qty
DY999
Ancillary Products Available
DY008B: DuoSet ELISA Ancillary Reagent Kit 2
Substrate Reagent Pack
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Product Details
Citations (114)
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Supplemental Products
Reviews (47)
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Substrate Reagent Pack Summary

INTENDED USE & DESCRIPTION

Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

REAGENT PREPARATION

Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

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Product Datasheet
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Substrate Reagent Pack

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

114 Citations: Showing 1 - 10
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  1. Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
    Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
    Scientific Reports, 2022;12(1):20622.  2022
  2. Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy
    Authors: M Fassler, S Tshori, Y Barac, DE Bowles, C Benaim, J George
    Biology, 2022;11(10):.  2022
  3. E-Cigarette Aerosols Promote Oral S. aureus Colonization by Delaying an Immune Response and Bacterial Clearing
    Authors: AR Cátala-Val, J Almeda, JN Bernard, AM Cole, AL Cole, SD Moore, CD Andl
    Cells, 2022;11(5):.  2022
  4. Altered IgG glycosylation at COVID-19 diagnosis predicts disease severity
    Authors: MM Vicente, I Alves, J Gaifem, CS Rodrigues, Â Fernandes, AM Dias, J Štambuk, T Petrovi?, P Oliveira, F Ferreira-d, A Soares, N Seixas, T Teixeira, L Malheiro, MM Abreu, G Lauc, R Sarmento E, SS Pinho
    European Journal of Immunology, 2022;0(0):.  2022
  5. Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells
    Authors: PK Yadav, P Haruehanro, S Irani, T Wang, A Ansari, J Sheng, MM Hussain
    The Journal of Biological Chemistry, 2022;0(0):101813.  2022
  6. Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent rats
    Authors: A Fatahi, E Zarrinkala, K Azizbeigi, K Ranjbar
    Experimental gerontology, 2022;162(0):111758.  2022
  7. A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
    Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
    Scientific Reports, 2022;12(1):15008.  2022
  8. Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model
    Authors: KT Liu, YN Gong, CG Huang, PN Huang, KY Yu, HC Lee, SC Lee, HJ Chiang, YA Kung, YT Lin, MJ Hsiao, PW Huang, SY Huang, HT Wu, CC Wu, RL Kuo, KF Chen, CT Hung, KY Oguntuyo, CS Stevens, S Kowdle, HP Chiu, B Lee, GW Chen, SR Shih
    mSphere, 2022;7(1):e0088321.  2022
  9. Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing
    Authors: H Jahns, R Degaonkar, P Podbevsek, S Gupta, A Bisbe, K Aluri, J Szeto, P Kumar, S LeBlanc, T Racie, CR Brown, A Castoreno, DC Guenther, V Jadhav, MA Maier, J Plavec, M Egli, M Manoharan, I Zlatev
    Nucleic Acids Research, 2021;0(0):.  2021
  10. Acetylation of Calmodulin Regulates Synaptic Plasticity and Fear Learning
    Authors: HL Zhang, B Zhao, W Han, YB Sun, P Yang, Y Chen, D Ni, J Zhang, DM Yin
    The Journal of Biological Chemistry, 2021;0(0):101034.  2021
  11. Effect of PLC-beta1/CaM signaling pathway mediated by AT1R on the occurrence and development of hepatocellular carcinoma
    Authors: ZW Xu, NN Liu, XY Wang, BC Ding, HF Zhang, Y Li, WY Sun, W Wei
    Cancer Cell International, 2021;21(1):587.  2021
  12. (-)-Carveol Prevents Gastric Ulcers via Cytoprotective, Antioxidant, Antisecretory and Immunoregulatory Mechanisms in Animal Models
    Authors: CAL Serafim, MEC Araruna, EB Alves Júni, LMO Silva, AO Silva, MS da Silva, AF Alves, AA Araújo, LM Batista
    Frontiers in Pharmacology, 2021;12(0):736829.  2021
  13. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex
    Authors: R González-P, K Eifler-Oli, LA Claessens, E Willemstei, Z Xiao, CMP Talavera O, H Ovaa, HD Ulrich, ACO Vertegaal
    Cell Reports, 2021;34(4):108691.  2021
  14. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
    Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
    Nature, 2021;0(0):.  2021
  15. Development of a Low-Molecular-Weight Abeta42 Detection System Using a Enzyme-Linked Peptide Assay
    Authors: SH Kim, EH Lee, HJ Kim, AR Kim, YE Kim, JH Lee, MY Yoon, SH Koh
    Biomolecules, 2021;11(12):.  2021
  16. LRG1 Expression Is Elevated in the Eyes of Patients with Neovascular Age-Related Macular Degeneration
    Authors: L Mundo, GM Tosi, S Lazzi, G Pertile, B Parolini, G Neri, M Posarelli, E De Benedet, T Bacci, E Silvestri, MC Siciliano, S Barbera, M Orlandini, J Greenwood, SE Moss, F Galvagni
    International Journal of Molecular Sciences, 2021;22(16):.  2021
  17. A simple detection method for the serum sFLT1 protein in preeclampsia
    Authors: M Shibuya, H Matsui, T Sasagawa, T Nagamatsu
    Scientific Reports, 2021;11(1):20613.  2021
  18. In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
    Authors: T Kishimoto, W Ishida, T Nasukawa, T Ujihara, I Nakajima, T Suzuki, J Uchiyama, D Todokoro, M Daibata, A Fukushima, S Matsuzaki, K Fukuda
    Microorganisms, 2021;9(2):.  2021
  19. &alpha-Phellandrene exhibits antinociceptive and tumor-reducing effects in a mouse model of oncologic pain
    Authors: FR Pinheiro-N, EM Lopes, BT Acha, LDS Gomes, WA Dias, ACD Reis Filho, BS Leal, DCDN Rodrigues, JDN Silva, D Dittz, PMP Ferreira, FRC Almeida
    Toxicology and Applied Pharmacology, 2021;418(0):115497.  2021
  20. Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
    Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
    Oncology Letters, 2021;22(5):780.  2021
  21. Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
    Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
    International Journal of Molecular Sciences, 2021;22(18):.  2021
  22. Short-term treatment of golden retriever muscular dystrophy (GRMD) dogs with rAAVrh74.MHCK7.GALGT2 induces muscle glycosylation and utrophin expression but has no significant effect on muscle strength
    Authors: PT Martin, DA Zygmunt, A Ashbrook, S Hamilton, D Packer, SM Birch, AK Bettis, CJ Balog-Alva, LJ Guo, PP Nghiem, JN Kornegay
    PLoS ONE, 2021;16(3):e0248721.  2021
  23. Serum IL-18 Is a Potential Biomarker for Predicting Severe Dengue Disease Progression
    Authors: JD Nanda, CJ Jung, RD Satria, MK Jhan, TJ Shen, PC Tseng, YT Wang, TS Ho, CF Lin
    Journal of Immunology Research, 2021;2021(0):7652569.  2021
  24. Double stranded RNA drives innate immune responses, sickness behavior and cognitive impairment dependent on dsRNA length, IFNAR1 expression and age
    Authors: N McGarry, C Murray, S Garvey, A Wilkinson, L Tortorelli, L Ryan, L Hayden, D Healy, EW Griffin, E Hennessy, DT Skelly, M Arumugam, KJ Mitchell, C Cunningham
    bioRxiv : the preprint server for biology, 2021;0(0):.  2021
  25. Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
    Authors: CD Hodge, DJ Rosenberg, M Wilamowski, A Joachimiak, GL Hura, M Hammel
    bioRxiv : the preprint server for biology, 2021;0(0):.  2021
  26. Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections
    Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
    Cell Rep, 2020;31(9):107725.  2020
  27. The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response
    Authors: S Lee, S Kim, HY Kang, HR Lim, Y Kwon, M Jo, YM Jeon, SR Kim, K Kim, CM Ha, S Lee, HJ Kim
    J Neuroinflammation, 2020;17(1):299.  2020
  28. SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia
    Authors: A Moutal, LF Martin, L Boinon, K Gomez, D Ran, Y Zhou, HJ Stratton, S Cai, S Luo, KB Gonzalez, S Perez-Mill, A Patwardhan, MM Ibrahim, R Khanna
    bioRxiv : the preprint server for biology, 2020;0(0):.  2020
  29. TRI microparticles prevent inflammatory arthritis in a collagen-induced arthritis model
    Authors: EJ Bassin, AR Buckley, JD Piganelli, SR Little
    PLoS ONE, 2020;15(9):e0239396.  2020
  30. Cross-reactive antibody response between SARS-CoV-2 and SARS-CoV infections
    Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
    Cell Rep, 2020;31(9):107725.  2020
  31. Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
    Authors: L Kang, S Smith, C Wang
    Journal of colloid and interface science, 2020;588(0):101-109.  2020
  32. Utility of Centrifugation-Controlled Convective (C3) Flow for Rapid On-chip ELISA
    Authors: W Espulgar, T Tadokoro, E Tamiya, M Saito
    Sci Rep, 2019;9(1):20150.  2019
  33. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019;8(7):.  2019
  34. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019;14(0):6117-6131.  2019
  35. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019;0(0):.  2019
  36. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019;27(11):3117-3123.e5.  2019
  37. Natural Killer Response and Lipo-Metabolic Profile in Adults with Low HDL-Cholesterol and Mild Hypercholesterolemia: Beneficial Effects of Artichoke Leaf Extract Supplementation
    Authors: M Rondanelli, AM Castellazz, A Riva, P Allegrini, MA Faliva, G Peroni, M Naso, M Nichetti, C Tagliacarn, C Valsecchi, T Fazia, S Perna, F Graziano, M Grassi, L Bernardine
    Evid Based Complement Alternat Med, 2019;2019(0):2069701.  2019
  38. IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis
    Authors: PJ Alcolea, A Alonso, A Esteban, P Peris, A Cortés, JA Castillo, V Larraga
    PLoS ONE, 2019;14(2):e0212136.  2019
  39. Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells
    Authors: JS Lim, GY Jung, SY Park
    J. Korean Med. Sci., 2019;34(12):e100.  2019
  40. GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites
    Authors: N Morita, E Umemoto, S Fujita, A Hayashi, J Kikuta, I Kimura, T Haneda, T Imai, A Inoue, H Mimuro, Y Maeda, H Kayama, R Okumura, J Aoki, N Okada, T Kida, M Ishii, R Nabeshima, K Takeda
    Nature, 2019;0(0):.  2019
  41. Infliximab Induced a Dissociated Response of Severe Periodontal Biomarkers in Rheumatoid Arthritis Patients
    Authors: M Rinaudo-Ga, V Blasco-Baq, P Miossec, P Gaudin, P Farge, X Roblin, T Thomas, S Paul, H Marotte
    J Clin Med, 2019;8(5):.  2019
  42. H7 Hemagglutinin nanoparticles retain immunogenicity after >3 months of 25�C storage
    Authors: TZ Chang, L Deng, BZ Wang, JA Champion
    PLoS ONE, 2018;13(8):e0202300.  2018
  43. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.  2018
  44. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4
    Authors: K Tzelepis, E De Braekel, D Aspris, I Barbieri, MS Vijayabask, WH Liu, M Gozdecka, E Metzakopia, HD Toop, M Dudek, SC Robson, F Hermida-Pr, YH Yang, R Babaei-Jad, DA Garyfallos, H Ponstingl, JML Dias, P Gallipoli, M Seiler, S Buonamici, B Vick, AJ Bannister, R Rad, RK Prinjha, JC Marioni, B Huntly, J Batson, JC Morris, C Pina, A Bradley, I Jeremias, DO Bates, K Yusa, T Kouzarides, GS Vassiliou
    Nat Commun, 2018;9(1):5378.  2018
  45. GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury
    Authors: A Benady, D Freidin, CG Pick, V Rubovitch
    Sci Rep, 2018;8(1):13340.  2018
  46. Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis
    Authors: Y Liu, OC Ndumnego, T Chen, RS Kim, ER Jenny-Avit, T Ndung'u, D Wilson, JM Achkar
    Pathogens, 2018;7(1):.  2018
  47. Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
    Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
    Adv Healthc Mater, 2018;0(0):.  2018
  48. Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal
    Authors: M Harhala, DC Nelson, P Miernikiew, RD Heselpoth, B Brzezicka, J Majewska, SB Linden, X Shang, A Szymczak, D Lecion, K Marek-Buko, M K?ak, B Wojciechow, K Lahutta, A Konieczny, K D?browska
    Viruses, 2018;10(11):.  2018
  49. Elevated Hsp90-beta contributes to differential diagnosis of pleural effusion caused by lung cancer and correlates with malignant biological behavior of lung cancer
    Authors: R Biaoxue, L Min, F Tian, G Wenlong, L Hua
    BMC Pulm Med, 2018;18(1):188.  2018
  50. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.  2018
  51. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018;0(0):.  2018
  52. Inhibition of anthrax lethal factor by ssDNA aptamers
    Authors: M Lahousse, HC Park, SC Lee, NR Ha, IP Jung, SR Schlesinge, K Shackelfor, MY Yoon, SK Kim
    Arch. Biochem. Biophys., 2018;646(0):16-23.  2018
  53. Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
    Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
    Int J Mol Sci, 2017;18(1):.  2017
  54. Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
    Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
    Adv Biosyst, 2017;1(9):1700047.  2017
  55. YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
    Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
    Sci Rep, 2017;7(1):11326.  2017
  56. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.  2017
  57. Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate
    Authors: RL Tallmadge, SC Miller, SA Parry, MJB Felippe
    PLoS ONE, 2017;12(5):e0177831.  2017
  58. Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors
    Authors: SA O'Sullivan, M Velasco-Es, KK Dev
    Glia, 2017;0(0):.  2017
  59. Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
    Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
    EBioMedicine, 2017;0(0):.  2017
  60. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury
    Authors: V Rubovitch, Y Zilberstei, J Chapman, S Schreiber, CG Pick
    Sci Rep, 2017;7(0):41269.  2017
  61. Lettuce-produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination
    Authors: J Liu Clarke, L Paruch, MO Dobrica, I Caras, C Tucureanu, A Onu, S Ciulean, C Stavaru, A Eerde, Y Wang, H Steen, S Haugslien, C Petrareanu, C Lazar, I Popescu, R Bock, J Dubuisson, N Branza-Nic
    Plant Biotechnol. J., 2017;0(0):.  2017
  62. Evaluation of multivalent H2 influenza pandemic vaccines in mice
    Authors: BJ Lenny, S Sonnberg, AF Danner, K Friedman, RJ Webby, RG Webster, JC Jones
    Vaccine, 2017;0(0):.  2017
  63. The ESRP1-GPR137 axis contributes to intestinal pathogenesis
    Authors: LF Mager, VH Koelzer, R Stuber, L Thoo, I Keller, I Koeck, M Langenegge, C Simillion, SP Pfister, M Faderl, V Genitsch, I Tcymbarevi, P Juillerat, X Li, Y Xia, E Karamitopo, R Lyck, I Zlobec, S Hapfelmeie, R Bruggmann, KD McCoy, AJ Macpherson, C Müller, B Beutler, P Krebs
    Elife, 2017;6(0):.  2017
  64. Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina
    PLoS ONE, 2016;11(8):e0161898.  2016
  65. Marked elevation in plasma osteoprotegerin constitutes an early and consistent feature of cerebral malaria
    Thromb. Haemost., 2016;115(4):773-80.  2016
  66. The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
    Nat Commun, 2016;7(0):13517.  2016
  67. Mechanism of the antigen-independent cytokinergic SPE-7 IgE activation of human mast cells in vitro.
    Authors: Bax H, Bowen H, Dodev T, Sutton B, Gould H
    Sci Rep, 2015;5(0):9538.  2015
  68. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
    Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
    PLoS ONE, 2015;10(9):e0137366.  2015
  69. An automated method for determining the cytoadhesion of Plasmodium falciparum-infected erythrocytes to immobilized cells.
    Authors: Hempel C, Boisen I, Efunshile A, Kurtzhals J, Staalso T
    Malar J, 2015;14(0):112.  2015
  70. Non-Human Primates Harbor Diverse Mammalian and Avian Astroviruses Including Those Associated with Human Infections.
    Authors: Karlsson E, Small C, Freiden P, Feeroz M, Matsen F, San S, Hasan M, Wang D, Jones-Engel L, Schultz-Cherry S
    PLoS Pathog, 2015;11(11):e1005225.  2015
  71. Innate immunity drives the initiation of a murine model of primary biliary cirrhosis.
    Authors: Chang C, Chen Y, Zhang W, Leung P, Gershwin M, Chuang Y
    PLoS ONE, 2015;10(3):e0121320.  2015
  72. Bone marrow-derived macrophages from aged rats are more responsive to inflammatory stimuli.
    Authors: Barrett J, Costello D, O'Sullivan J, Cowley T, Lynch M
    J Neuroinflammation, 2015;12(0):67.  2015
  73. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.  2015
  74. Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
    Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
    J Interferon Cytokine Res, 2015;35(1):42-8.  2015
  75. A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.
    Authors: Riebold M, Kozany C, Freiburger L, Sattler M, Buchfelder M, Hausch F, Stalla G, Paez-Pereda M
    Nat Med, 2015;21(3):276-80.  2015
  76. Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
    Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
    J Transl Med, 2015;13(0):191.  2015
  77. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.  2015
  78. Detection of antibodies against Turkey astrovirus in humans.
    Authors: Meliopoulos V, Kayali G, Burnham A, Oshansky C, Thomas P, Gray G, Beck M, Schultz-Cherry S
    PLoS ONE, 2014;9(5):e96934.  2014
  79. Netrin-1 controls sympathetic arterial innervation.
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  87. Hepatocytes induce Foxp3(+) regulatory T cells by Notch signaling.
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    J Leukoc Biol, 2014;96(4):571-7.  2014
  88. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
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  89. Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.
    Authors: Noda K, Dabovic B, Takagi K, Inoue T, Horiguchi M, Hirai M, Fujikawa Y, Akama T, Kusumoto K, Zilberberg L, Sakai L, Koli K, Naitoh M, von Melchner H, Suzuki S, Rifkin D, Nakamura T
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  90. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
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    PLoS Pathog, 2013;9(12):e1003852.  2013
  91. Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer.
    Authors: Fu, Le, Guo, Wei, Liu, Bingshan, Sun, Linlin, Bi, Zhenghon, Zhu, Li, Wang, Xinyan, Liu, Bin, Xie, Qian, Li, Ke
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  92. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
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    Circ Heart Fail, 2011;4(2):114-20.  2011
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DuoSet Ancillary Reagent Kits

DuoSet ELISA Ancillary Reagent Kit 2

DY008B
76 Citations
2 Reviews
ELISA

Reviews for Substrate Reagent Pack

Average Rating: 4.7 (Based on 47 Reviews)

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Substrate Reagent Pack
By Anonymous on 02/27/2023
Substrate Reagent Pack
By Anonymous on 05/23/2022

Substrate works well in direct and sandwich ELISAs

Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 05/13/2021
Substrate Reagent Pack
By Anonymous on 01/12/2021
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 12/17/2020
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Gagandeep Kaur on 11/11/2020
Substrate Reagent Pack
By Walker McHugh on 10/23/2020
Substrate Reagent Pack
By Anonymous on 08/11/2020
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Kumudhini Haran on 07/13/2020
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 06/20/2020
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 02/11/2020
Substrate Reagent Pack
By Anonymous on 01/17/2020
Substrate Reagent Pack
By Anonymous on 01/10/2020
Substrate Reagent Pack
By Tahmineh Safaie on 12/23/2019

One pair of A and B is enough for one plate which makes it very easy to use while keep the reagent stable

Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 09/07/2019
Substrate Reagent Pack
By Anonymous on 07/02/2019
Substrate Reagent Pack
By Anonymous on 03/28/2019

I used this a my detection substrate (100uL) in a direct ELISA after my plate was treated with an anti-mouse IgG-HRP antibody. I find the background is very low which allows me to achieved a better sensitivity with my ELISAs compared another commercial substrate

Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 03/13/2019
Substrate Reagent Pack
By Ivan Luzardo on 01/03/2019
Substrate Reagent Pack
By Anonymous on 12/10/2018
Substrate Reagent Pack
By Marry Tahani on 11/21/2018

It is very stable before mixing the reagent A and B. One bottle of each is exactly enough for two plates which makes measuring simple.

Substrate Reagent Pack DY999
Substrate Reagent Pack
By Sijia He on 11/13/2018
Substrate Reagent Pack
By Anonymous on 09/30/2018
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 08/17/2018
Substrate Reagent Pack
By Anonymous on 06/19/2018
Substrate Reagent Pack
By Anonymous on 06/15/2018
Substrate Reagent Pack
By Anonymous on 05/10/2018
Substrate Reagent Pack
By Leslie Priddy on 03/30/2018
Substrate Reagent Pack
By Anonymous on 02/22/2018
Substrate Reagent Pack
By Anonymous on 02/06/2018
Substrate Reagent Pack
By Anonymous on 02/06/2018
Substrate Reagent Pack DY999
Substrate Reagent Pack
By Anonymous on 01/19/2018
Substrate Reagent Pack
By Balaji Mahender on 12/20/2017
Substrate Reagent Pack
By Jomkuan Theprungsirikul on 12/15/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Jomkuan Theprungsirikul on 12/05/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By David Bolick on 09/07/2017

Our lab does many ELISAs. These substrate solutions are excellent, affordable, and easy to use.

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/19/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/17/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/14/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/06/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Goutham Pattabiraman on 06/15/2017

Really Easy to use.
After the SA-HRP detection step for ELISA, wash wells 4 times with wash buffer, and add the Substrate and incubate for 20 mins in the dark. Stop the reaction using 0.2 M sulfuric acid. Read the plate at 450nm

Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Abby Sukarto on 05/03/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 03/29/2017
Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 11/28/2016
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Adam Guess on 09/01/2016
Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By John Yu on 04/26/2016
Substrate Reagent Pack (8 vials Color A, 8 vials Color B) DY999
Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 01/18/2016