Substrate Reagent Pack

In alignment with Bio-Techne’s commitment to sustainability and Go Green initiatives, we will no longer pack paper kit booklets in the following immunoassay kits:

  • Quantikine® RUO ELISA Kits                                   
  • Quantikine® HS ELISA Kits
  • QuantiGloELISA Kits
  • QuicKitELISA Kits
  • Fluorokine Kits
  • Parameter Assay Kits
  • ELISpot Kits
Newer Version Available: DY999B
NEW
8 vials Color A, 8 vials Color B
Catalog # Availability Size / Price Qty
DY999
Ancillary Products Available

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Substrate Reagent Pack
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Product Details
Citations (129)
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Supplemental Products
Reviews (49)

Substrate Reagent Pack Summary

INTENDED USE & DESCRIPTION

Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

REAGENT PREPARATION

Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Substrate Reagent Pack

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

129 Citations: Showing 1 - 10
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  1. Alpha-7 Nicotinic Acetylcholine Receptor Activation Inhibits Trauma Induced Pronociceptive Autoimmune Responses
    Authors: Li, WW;Shi, XY;Wei, T;Guo, TZ;Kingery, WS;David Clark, J;
    The journal of pain  2023-11-09
  2. LncRNA Neat1 targets NonO and miR-128-3p to promote antigen-specific Th17 cell responses and autoimmune inflammation
    Authors: Chen, S;Wang, J;Zhang, K;Ma, B;Li, X;Wei, R;Nian, H;
    Cell death & disease  2023-09-16
  3. Chitosan Resin-Modified Glass Ionomer Cement Containing Epidermal Growth Factor Promotes Pulp Cell Proliferation with a Minimum Effect on Fluoride and Aluminum Release
    Authors: Hengtrakool, C;Wanichpakorn, S;Kedjarune-Leggat, U;
    Polymers  2023-08-23
  4. Increased neutralization potency and breadth elicited by a SARS-CoV-2 mRNA vaccine forming virus-like particles
    Authors: Zhang, P;Falcone, S;Tsybovsky, Y;Singh, M;Gopan, V;Miao, H;Seo, Y;Rogers, D;Renzi, I;Lai, YT;Narayanan, E;Stewart-Jones, G;Himansu, S;Carfi, A;Fauci, AS;Lusso, P;
    Proceedings of the National Academy of Sciences of the United States of America  2023-07-18
  5. In vivo evolution of a Klebsiella pneumoniae capsule defect promotes complement-mediated opsono-phagocytosis and persistence during recurrent infection
    Authors: Bain, W;Ahn, B;Penaloza, H;McElheny, C;Tolman, N;van der Geest, R;Gonzalez-Ferrer, S;Chen, N;An, X;Hosuru, R;Tabary, M;Papke, E;Kohli, N;Farooq, N;Bachman, W;Olonisakin, T;Xiong, Z;Griffith, MP;Sullivan, M;Franks, J;Mustapha, M;Iovleva, A;Suber, T;Shanks, RM;Ferreira, V;Stolz, DB;Van Tyne, D;Doi, Y;Lee, J;
    bioRxiv : the preprint server for biology  2023-06-01
  6. MANF regulates neuronal survival and UPR through its ER-located receptor IRE1alpha
    Authors: V Kovaleva, LY Yu, L Ivanova, O Shpironok, J Nam, A Eesmaa, EP Kumpula, S Sakson, U Toots, M Ustav, JT Huiskonen, MH Voutilaine, P Lindholm, M Karelson, M Saarma
    Cell Reports, 2023-02-03;42(2):112066.  2023-02-03
  7. Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
    Authors: S Choi, Z Yang, Q Wang, Z Qiao, M Sun, J Wiggins, SH Xiang, Q Lu
    Science Advances, 2023-01-27;9(4):eade2708.  2023-01-27
  8. Does the Blood-Brain Barrier Integrity Change in Regard to the Onset of Fetal Growth Restriction?
    Authors: N Misan, S Michalak, K Kapska, K Osztynowic, M Ropacka-Le, K Kawka-Paci
    International Journal of Molecular Sciences, 2023-01-19;24(3):.  2023-01-19
  9. Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma
    Authors: MJ Rodrigo, I Bravo-Osun, M Subias, A Montolío, J Cegoñino, T Martinez-R, S Mendez-Mar, A Aragón-Nav, D Garcia-Her, LE Pablo, R Herrero-Va, AP Del Paloma, E Garcia-Mar
    Scientific Reports, 2022-11-30;12(1):20622.  2022-11-30
  10. Dual Targeting of Soluble Oligomeric and Aggregated Transthyretin with a Monoclonal Antibody Ameliorates Experimental Neuropathy
    Authors: M Fassler, S Tshori, Y Barac, DE Bowles, C Benaim, J George
    Biology, 2022-10-15;11(10):.  2022-10-15
  11. A TGFbetaR inhibitor represses keratin-7 expression in 3D cultures of human salivary gland progenitor cells
    Authors: EW Fowler, EJ van Venroo, RL Witt, X Jia
    Scientific Reports, 2022-09-02;12(1):15008.  2022-09-02
  12. A pilot study on nitration/dysfunction of NK1 segment of myogenic stem cell activator HGF
    Authors: A Elgaabari, N Imatomi, H Kido, M Seki, S Tanaka, Y Matsuyoshi, T Nakashima, S Sawano, W Mizunoya, T Suzuki, M Nakamura, JE Anderson, R Tatsumi
    Biochemistry and Biophysics Reports, 2022-06-11;31(0):101295.  2022-06-11
  13. Altered IgG glycosylation at COVID-19 diagnosis predicts disease severity
    Authors: MM Vicente, I Alves, J Gaifem, CS Rodrigues, Â Fernandes, AM Dias, J Štambuk, T Petrovi?, P Oliveira, F Ferreira-d, A Soares, N Seixas, T Teixeira, L Malheiro, MM Abreu, G Lauc, R Sarmento E, SS Pinho
    European Journal of Immunology, 2022-04-04;0(0):.  2022-04-04
  14. Novel efficacious microRNA-30c analogs reduce apolipoprotein B secretion in human hepatoma and primary hepatocyte cells
    Authors: PK Yadav, P Haruehanro, S Irani, T Wang, A Ansari, J Sheng, MM Hussain
    The Journal of Biological Chemistry, 2022-03-10;0(0):101813.  2022-03-10
  15. Cardioprotective effects of exercise preconditioning on ischemia-reperfusion injury and ventricular ectopy in young and senescent rats
    Authors: A Fatahi, E Zarrinkala, K Azizbeigi, K Ranjbar
    Experimental gerontology, 2022-03-02;162(0):111758.  2022-03-02
  16. E-Cigarette Aerosols Promote Oral S. aureus Colonization by Delaying an Immune Response and Bacterial Clearing
    Authors: AR Cátala-Val, J Almeda, JN Bernard, AM Cole, AL Cole, SD Moore, CD Andl
    Cells, 2022-02-23;11(5):.  2022-02-23
  17. Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model
    Authors: KT Liu, YN Gong, CG Huang, PN Huang, KY Yu, HC Lee, SC Lee, HJ Chiang, YA Kung, YT Lin, MJ Hsiao, PW Huang, SY Huang, HT Wu, CC Wu, RL Kuo, KF Chen, CT Hung, KY Oguntuyo, CS Stevens, S Kowdle, HP Chiu, B Lee, GW Chen, SR Shih
    mSphere, 2022-02-02;7(1):e0088321.  2022-02-02
  18. Development of a Low-Molecular-Weight Abeta42 Detection System Using a Enzyme-Linked Peptide Assay
    Authors: SH Kim, EH Lee, HJ Kim, AR Kim, YE Kim, JH Lee, MY Yoon, SH Koh
    Biomolecules, 2021-12-02;11(12):.  2021-12-02
  19. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion
    Authors: X Sun, B Wu, HC Chiang, H Deng, X Zhang, W Xiong, J Liu, AM Rozeboom, BT Harris, E Blommaert, A Gomez, RE Garcia, Y Zhou, P Mitra, M Prevost, D Zhang, D Banik, C Isaacs, D Berry, C Lai, K Chaldekas, PS Latham, CA Brantner, A Popratilof, VX Jin, N Zhang, Y Hu, MA Pujana, TJ Curiel, Z An, R Li
    Nature, 2021-11-03;0(0):.  2021-11-03
  20. Effect of PLC-beta1/CaM signaling pathway mediated by AT1R on the occurrence and development of hepatocellular carcinoma
    Authors: ZW Xu, NN Liu, XY Wang, BC Ding, HF Zhang, Y Li, WY Sun, W Wei
    Cancer Cell International, 2021-11-02;21(1):587.  2021-11-02
  21. Serum IL-18 Is a Potential Biomarker for Predicting Severe Dengue Disease Progression
    Authors: JD Nanda, CJ Jung, RD Satria, MK Jhan, TJ Shen, PC Tseng, YT Wang, TS Ho, CF Lin
    Journal of Immunology Research, 2021-10-25;2021(0):7652569.  2021-10-25
  22. A simple detection method for the serum sFLT1 protein in preeclampsia
    Authors: M Shibuya, H Matsui, T Sasagawa, T Nagamatsu
    Scientific Reports, 2021-10-18;11(1):20613.  2021-10-18
  23. Small circular interfering RNAs (sciRNAs) as a potent therapeutic platform for gene-silencing
    Authors: H Jahns, R Degaonkar, P Podbevsek, S Gupta, A Bisbe, K Aluri, J Szeto, P Kumar, S LeBlanc, T Racie, CR Brown, A Castoreno, DC Guenther, V Jadhav, MA Maier, J Plavec, M Egli, M Manoharan, I Zlatev
    Nucleic Acids Research, 2021-10-11;0(0):.  2021-10-11
  24. Identification of soluble tissue-derived biomarkers from human thyroid tissue explants maintained on a microfluidic device
    Authors: A Riley, H Jones, J England, D Kuvshinov, V Green, J Greenman
    Oncology Letters, 2021-09-13;22(5):780.  2021-09-13
  25. Human-Specific Regulation of Neurotrophic Factors MANF and CDNF by microRNAs
    Authors: J Konovalova, D Gerasymchu, SN Arroyo, S Kluske, F Mastroiann, AV Pereyra, A Domanskyi
    International Journal of Molecular Sciences, 2021-09-07;22(18):.  2021-09-07
  26. (-)-Carveol Prevents Gastric Ulcers via Cytoprotective, Antioxidant, Antisecretory and Immunoregulatory Mechanisms in Animal Models
    Authors: CAL Serafim, MEC Araruna, EB Alves Júni, LMO Silva, AO Silva, MS da Silva, AF Alves, AA Araújo, LM Batista
    Frontiers in Pharmacology, 2021-08-23;12(0):736829.  2021-08-23
  27. LRG1 Expression Is Elevated in the Eyes of Patients with Neovascular Age-Related Macular Degeneration
    Authors: L Mundo, GM Tosi, S Lazzi, G Pertile, B Parolini, G Neri, M Posarelli, E De Benedet, T Bacci, E Silvestri, MC Siciliano, S Barbera, M Orlandini, J Greenwood, SE Moss, F Galvagni
    International Journal of Molecular Sciences, 2021-08-18;22(16):.  2021-08-18
  28. Acetylation of Calmodulin Regulates Synaptic Plasticity and Fear Learning
    Authors: HL Zhang, B Zhao, W Han, YB Sun, P Yang, Y Chen, D Ni, J Zhang, DM Yin
    The Journal of Biological Chemistry, 2021-07-31;0(0):101034.  2021-07-31
  29. Short-term treatment of golden retriever muscular dystrophy (GRMD) dogs with rAAVrh74.MHCK7.GALGT2 induces muscle glycosylation and utrophin expression but has no significant effect on muscle strength
    Authors: PT Martin, DA Zygmunt, A Ashbrook, S Hamilton, D Packer, SM Birch, AK Bettis, CJ Balog-Alva, LJ Guo, PP Nghiem, JN Kornegay
    PLoS ONE, 2021-03-26;16(3):e0248721.  2021-03-26
  30. &alpha-Phellandrene exhibits antinociceptive and tumor-reducing effects in a mouse model of oncologic pain
    Authors: FR Pinheiro-N, EM Lopes, BT Acha, LDS Gomes, WA Dias, ACD Reis Filho, BS Leal, DCDN Rodrigues, JDN Silva, D Dittz, PMP Ferreira, FRC Almeida
    Toxicology and Applied Pharmacology, 2021-03-17;418(0):115497.  2021-03-17
  31. Global non-covalent SUMO interaction networks reveal SUMO-dependent stabilization of the non-homologous end joining complex
    Authors: R González-P, K Eifler-Oli, LA Claessens, E Willemstei, Z Xiao, CMP Talavera O, H Ovaa, HD Ulrich, ACO Vertegaal
    Cell Reports, 2021-01-26;34(4):108691.  2021-01-26
  32. In Vitro and In Vivo Evaluation of Three Newly Isolated Bacteriophage Candidates, phiEF7H, phiEF14H1, phiEF19G, for Treatment of Enterococcus faecalis Endophthalmitis
    Authors: T Kishimoto, W Ishida, T Nasukawa, T Ujihara, I Nakajima, T Suzuki, J Uchiyama, D Todokoro, M Daibata, A Fukushima, S Matsuzaki, K Fukuda
    Microorganisms, 2021-01-20;9(2):.  2021-01-20
  33. Rigid monoclonal antibodies improve detection of SARS-CoV-2 nucleocapsid protein
    Authors: CD Hodge, DJ Rosenberg, M Wilamowski, A Joachimiak, GL Hura, M Hammel
    bioRxiv : the preprint server for biology, 2021-01-13;0(0):.  2021-01-13
  34. Double stranded RNA drives innate immune responses, sickness behavior and cognitive impairment dependent on dsRNA length, IFNAR1 expression and age
    Authors: N McGarry, C Murray, S Garvey, A Wilkinson, L Tortorelli, L Ryan, L Hayden, D Healy, EW Griffin, E Hennessy, DT Skelly, M Arumugam, KJ Mitchell, C Cunningham
    bioRxiv : the preprint server for biology, 2021-01-09;0(0):.  2021-01-09
  35. Stabilization of surface-bound antibodies for ELISA based on a reversable zeolitic imidazolate framework-8 coating
    Authors: L Kang, S Smith, C Wang
    Journal of colloid and interface science, 2020-12-24;588(0):101-109.  2020-12-24
  36. Rapid production of SARS-CoV-2 receptor binding domain (RBD) and spike specific monoclonal antibody CR3022 in Nicotiana benthamiana
    Authors: K Rattanapis, B Shanmugara, S Manopwised, PB Purwono, K Siriwattan, N Khorattana, O Hanittinan, W Boonyayoth, A Thitithany, DR Smith, W Phoolcharo
    Sci Rep, 2020-10-19;10(1):17698.  2020-10-19
  37. The overexpression of TDP-43 in astrocytes causes neurodegeneration via a PTP1B-mediated inflammatory response
    Authors: S Lee, S Kim, HY Kang, HR Lim, Y Kwon, M Jo, YM Jeon, SR Kim, K Kim, CM Ha, S Lee, HJ Kim
    J Neuroinflammation, 2020-10-14;17(1):299.  2020-10-14
  38. TRI microparticles prevent inflammatory arthritis in a collagen-induced arthritis model
    Authors: EJ Bassin, AR Buckley, JD Piganelli, SR Little
    PLoS ONE, 2020-09-23;15(9):e0239396.  2020-09-23
  39. SARS-CoV-2 Spike protein co-opts VEGF-A/Neuropilin-1 receptor signaling to induce analgesia
    Authors: A Moutal, LF Martin, L Boinon, K Gomez, D Ran, Y Zhou, HJ Stratton, S Cai, S Luo, KB Gonzalez, S Perez-Mill, A Patwardhan, MM Ibrahim, R Khanna
    bioRxiv : the preprint server for biology, 2020-09-14;0(0):.  2020-09-14
  40. Cross-reactive antibody response between SARS-CoV-2 and SARS-CoV infections
    Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
    Cell Rep, 2020-05-18;31(9):107725.  2020-05-18
  41. Cross-reactive Antibody Response between SARS-CoV-2 and SARS-CoV Infections
    Authors: H Lv, NC Wu, OT Tsang, M Yuan, RAPM Perera, WS Leung, RTY So, JMC Chan, GK Yip, TSH Chik, Y Wang, CYC Choi, Y Lin, WW Ng, J Zhao, LLM Poon, JSM Peiris, IA Wilson, CKP Mok
    Cell Rep, 2020-01-01;31(9):107725.  2020-01-01
  42. Utility of Centrifugation-Controlled Convective (C3) Flow for Rapid On-chip ELISA
    Authors: W Espulgar, T Tadokoro, E Tamiya, M Saito
    Sci Rep, 2019-12-27;9(1):20150.  2019-12-27
  43. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019-08-01;14(0):6117-6131.  2019-08-01
  44. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019-07-25;0(0):.  2019-07-25
  45. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019-07-15;8(7):.  2019-07-15
  46. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019-06-11;27(11):3117-3123.e5.  2019-06-11
  47. Infliximab Induced a Dissociated Response of Severe Periodontal Biomarkers in Rheumatoid Arthritis Patients
    Authors: M Rinaudo-Ga, V Blasco-Baq, P Miossec, P Gaudin, P Farge, X Roblin, T Thomas, S Paul, H Marotte
    J Clin Med, 2019-05-26;8(5):.  2019-05-26
  48. Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells
    Authors: JS Lim, GY Jung, SY Park
    J. Korean Med. Sci., 2019-03-19;34(12):e100.  2019-03-19
  49. IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis
    Authors: PJ Alcolea, A Alonso, A Esteban, P Peris, A Cortés, JA Castillo, V Larraga
    PLoS ONE, 2019-02-22;14(2):e0212136.  2019-02-22
  50. GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites
    Authors: N Morita, E Umemoto, S Fujita, A Hayashi, J Kikuta, I Kimura, T Haneda, T Imai, A Inoue, H Mimuro, Y Maeda, H Kayama, R Okumura, J Aoki, N Okada, T Kida, M Ishii, R Nabeshima, K Takeda
    Nature, 2019-01-23;0(0):.  2019-01-23
  51. Natural Killer Response and Lipo-Metabolic Profile in Adults with Low HDL-Cholesterol and Mild Hypercholesterolemia: Beneficial Effects of Artichoke Leaf Extract Supplementation
    Authors: M Rondanelli, AM Castellazz, A Riva, P Allegrini, MA Faliva, G Peroni, M Naso, M Nichetti, C Tagliacarn, C Valsecchi, T Fazia, S Perna, F Graziano, M Grassi, L Bernardine
    Evid Based Complement Alternat Med, 2019-01-06;2019(0):2069701.  2019-01-06
  52. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4
    Authors: K Tzelepis, E De Braekel, D Aspris, I Barbieri, MS Vijayabask, WH Liu, M Gozdecka, E Metzakopia, HD Toop, M Dudek, SC Robson, F Hermida-Pr, YH Yang, R Babaei-Jad, DA Garyfallos, H Ponstingl, JML Dias, P Gallipoli, M Seiler, S Buonamici, B Vick, AJ Bannister, R Rad, RK Prinjha, JC Marioni, B Huntly, J Batson, JC Morris, C Pina, A Bradley, I Jeremias, DO Bates, K Yusa, T Kouzarides, GS Vassiliou
    Nat Commun, 2018-12-19;9(1):5378.  2018-12-19
  53. Elevated Hsp90-beta contributes to differential diagnosis of pleural effusion caused by lung cancer and correlates with malignant biological behavior of lung cancer
    Authors: R Biaoxue, L Min, F Tian, G Wenlong, L Hua
    BMC Pulm Med, 2018-12-06;18(1):188.  2018-12-06
  54. Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal
    Authors: M Harhala, DC Nelson, P Miernikiew, RD Heselpoth, B Brzezicka, J Majewska, SB Linden, X Shang, A Szymczak, D Lecion, K Marek-Buko, M K?ak, B Wojciechow, K Lahutta, A Konieczny, K D?browska
    Viruses, 2018-11-15;10(11):.  2018-11-15
  55. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 2018-10-10;156(1):74-85.  2018-10-10
  56. GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury
    Authors: A Benady, D Freidin, CG Pick, V Rubovitch
    Sci Rep, 2018-09-06;8(1):13340.  2018-09-06
  57. H7 Hemagglutinin nanoparticles retain immunogenicity after >3 months of 25�C storage
    Authors: TZ Chang, L Deng, BZ Wang, JA Champion
    PLoS ONE, 2018-08-09;13(8):e0202300.  2018-08-09
  58. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018-07-09;8(1):10328.  2018-07-09
  59. Inhibition of anthrax lethal factor by ssDNA aptamers
    Authors: M Lahousse, HC Park, SC Lee, NR Ha, IP Jung, SR Schlesinge, K Shackelfor, MY Yoon, SK Kim
    Arch. Biochem. Biophys., 2018-03-24;646(0):16-23.  2018-03-24
  60. Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis
    Authors: Y Liu, OC Ndumnego, T Chen, RS Kim, ER Jenny-Avit, T Ndung'u, D Wilson, JM Achkar
    Pathogens, 2018-02-27;7(1):.  2018-02-27
  61. Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
    Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
    Adv Healthc Mater, 2018-02-14;0(0):.  2018-02-14
  62. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018-02-01;2018(0):8286067.  2018-02-01
  63. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018-01-08;0(0):.  2018-01-08
  64. Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
    Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
    EBioMedicine, 2017-12-07;0(0):.  2017-12-07
  65. The ESRP1-GPR137 axis contributes to intestinal pathogenesis
    Authors: LF Mager, VH Koelzer, R Stuber, L Thoo, I Keller, I Koeck, M Langenegge, C Simillion, SP Pfister, M Faderl, V Genitsch, I Tcymbarevi, P Juillerat, X Li, Y Xia, E Karamitopo, R Lyck, I Zlobec, S Hapfelmeie, R Bruggmann, KD McCoy, AJ Macpherson, C Müller, B Beutler, P Krebs
    Elife, 2017-10-04;6(0):.  2017-10-04
  66. YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
    Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
    Sci Rep, 2017-09-12;7(1):11326.  2017-09-12
  67. Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
    Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
    Adv Biosyst, 2017-08-10;1(9):1700047.  2017-08-10
  68. Lettuce-produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination
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    Plant Biotechnol. J., 2017-06-09;0(0):.  2017-06-09
  69. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
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  70. Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate
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  71. Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors
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    Glia, 2017-04-04;0(0):.  2017-04-04
  72. Evaluation of multivalent H2 influenza pandemic vaccines in mice
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    Vaccine, 2017-02-08;0(0):.  2017-02-08
  73. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury
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  79. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
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  80. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
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  81. Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
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  82. Mechanism of the antigen-independent cytokinergic SPE-7 IgE activation of human mast cells in vitro.
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  83. Bone marrow-derived macrophages from aged rats are more responsive to inflammatory stimuli.
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  87. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
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    J Biol Chem, 2014-07-14;289(36):24922-35.  2014-07-14
  90. Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
    Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
    J Interferon Cytokine Res, 2014-07-09;35(1):42-8.  2014-07-09
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    PLoS ONE, 2014-07-03;9(7):e101715.  2014-07-03
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    J Leukoc Biol, 2014-06-26;96(4):571-7.  2014-06-26
  94. Dimerization is not a determining factor for functional high affinity human plasminogen binding by the group A streptococcal virulence factor PAM and is mediated by specific residues within the PAM a1a2 domain.
    Authors: Bhattacharya S, Liang Z, Quek A, Ploplis V, Law R, Castellino F
    J Biol Chem, 2014-06-24;289(31):21684-93.  2014-06-24
  95. Netrin-1 controls sympathetic arterial innervation.
    Authors: Brunet I, Gordon E, Han J, Cristofaro B, Broqueres-You D, Liu C, Bouvree K, Zhang J, Del Toro R, Mathivet T, Larrivee B, Jagu J, Pibouin-Fragner L, Pardanaud L, Machado M, Kennedy T, Zhuang Z, Simons M, Levy B, Tessier-Lavigne M, Grenz A, Eltzschig H, Eichmann A
    J Clin Invest, 2014-06-17;124(7):3230-40.  2014-06-17
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    PLoS ONE, 2014-05-14;9(5):e96934.  2014-05-14
  97. A soluble form of IL-27Ralpha is a natural IL-27 antagonist.
    Authors: Dietrich C, Candon S, Ruemmele F, Devergne O
    J Immunol, 2014-04-25;192(11):5382-9.  2014-04-25
  98. Alloimmune activation promotes anti-cancer cytotoxicity after rat liver transplantation.
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    PLoS ONE, 2014-03-20;9(3):e91515.  2014-03-20
  99. Nonfunctional variant 3 factor H binding proteins as meningococcal vaccine candidates.
    Authors: van der Veen, Stijn, Johnson, Steven, Jongerius, Ilse, Malik, Talat, Genovese, Alessia, Santini, Laura, Staunton, David, Ufret-Vincenty, Rafael L, Pickering, Matthew, Lea, Susan M, Tang, Christop
    Infect Immun, 2013-12-30;82(3):1157-63.  2013-12-30
  100. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013-12-26;9(12):e1003852.  2013-12-26
  101. GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2.
    Authors: Yang L, Friedland S, Corson N, Xu L
    Cancer Res, 2013-12-19;74(4):1022-31.  2013-12-19
  102. Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
    Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
    EMBO Mol Med, 2013-12-15;6(1):27-42.  2013-12-15
  103. Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer.
    Authors: Fu, Le, Guo, Wei, Liu, Bingshan, Sun, Linlin, Bi, Zhenghon, Zhu, Li, Wang, Xinyan, Liu, Bin, Xie, Qian, Li, Ke
    Biomarkers, 2013-03-01;18(2):126-35.  2013-03-01
  104. Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.
    Authors: Noda K, Dabovic B, Takagi K, Inoue T, Horiguchi M, Hirai M, Fujikawa Y, Akama T, Kusumoto K, Zilberberg L, Sakai L, Koli K, Naitoh M, von Melchner H, Suzuki S, Rifkin D, Nakamura T
    Proc Natl Acad Sci U S A, 2013-02-04;110(8):2852-7.  2013-02-04
  105. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012-06-20;7(6):e39036.  2012-06-20
  106. A novel role for insulin resistance in the connection between obesity and postmenopausal breast cancer.
    Authors: Weichhaus M, Broom J, Wahle K, Bermano G
    Int. J. Oncol., 2012-05-16;41(2):745-52.  2012-05-16
  107. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012-02-29;7(2):e32125.  2012-02-29
  108. Adiponectin inhibits leptin signalling via multiple mechanisms to exert protective effects against hepatic fibrosis.
    Authors: Handy JA, Fu PP, Kumar P
    Biochem. J., 2011-12-15;440(3):385-95.  2011-12-15
  109. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011-10-21;187(11):5745-55.  2011-10-21
  110. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011-04-19;19(10):1793-801.  2011-04-19
  111. Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
    Authors: Sakamoto N, Rosenberg AS
    J. Immunol., 2011-03-14;186(8):5004-11.  2011-03-14
  112. Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide.
    Authors: Ibebuogu UN, Gladysheva IP, Houng AK
    Circ Heart Fail, 2011-01-07;4(2):114-20.  2011-01-07
  113. Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
    PLoS ONE, 2010-10-21;5(10):e13568.  2010-10-21
  114. PEGylation of interleukin-10 for the mitigation of enhanced pain states.
    Authors: Soderquist RG, Milligan ED, Harrison JA, Chavez RA, Johnson KW, Watkins LR, Mahoney MJ
    J Biomed Mater Res A, 2010-06-01;93(3):1169-79.  2010-06-01
  115. Enzyme-linked enzyme-binding assay for Pin1 WW domain ligands.
    Authors: Mercedes-Camacho AY, Etzkorn FA
    Anal. Biochem., 2010-03-15;402(1):77-82.  2010-03-15
  116. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
    Vaccine, 2010-02-23;28(8):1942-51.  2010-02-23
  117. A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.
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  118. Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
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Reviews for Substrate Reagent Pack

Average Rating: 4.8 (Based on 49 Reviews)

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Substrate Reagent Pack
By Anonymous on 06/23/2023

Substrate Reagent Pack
By Do-Hyun Kim on 03/28/2023

Substrate Reagent Pack
By Anonymous on 02/27/2023

Substrate Reagent Pack
By Anonymous on 05/23/2022

Substrate works well in direct and sandwich ELISAs


Substrate Reagent Pack
By Anonymous on 05/13/2021

Substrate Reagent Pack
By Anonymous on 01/12/2021

Substrate Reagent Pack
By Anonymous on 12/17/2020

Substrate Reagent Pack
By Gagandeep Kaur on 11/11/2020

Substrate Reagent Pack
By Walker McHugh on 10/23/2020

Substrate Reagent Pack
By Anonymous on 08/11/2020

Substrate Reagent Pack
By Kumudhini Haran on 07/13/2020

Substrate Reagent Pack
By Anonymous on 06/20/2020

Substrate Reagent Pack
By Anonymous on 02/11/2020

Substrate Reagent Pack
By Anonymous on 01/17/2020

Substrate Reagent Pack
By Anonymous on 01/10/2020

Substrate Reagent Pack
By Tahmineh Safaie on 12/23/2019

One pair of A and B is enough for one plate which makes it very easy to use while keep the reagent stable


Substrate Reagent Pack
By Anonymous on 09/07/2019

Substrate Reagent Pack
By Anonymous on 07/02/2019

Substrate Reagent Pack
By Anonymous on 03/28/2019

I used this a my detection substrate (100uL) in a direct ELISA after my plate was treated with an anti-mouse IgG-HRP antibody. I find the background is very low which allows me to achieved a better sensitivity with my ELISAs compared another commercial substrate


Substrate Reagent Pack
By Anonymous on 03/13/2019

Substrate Reagent Pack
By Ivan Luzardo on 01/03/2019

Substrate Reagent Pack
By Anonymous on 12/10/2018

Substrate Reagent Pack
By Marry Tahani on 11/21/2018

It is very stable before mixing the reagent A and B. One bottle of each is exactly enough for two plates which makes measuring simple.


Substrate Reagent Pack
By Sijia He on 11/13/2018

Substrate Reagent Pack
By Anonymous on 09/30/2018

Substrate Reagent Pack
By Anonymous on 08/17/2018

Substrate Reagent Pack
By Anonymous on 06/19/2018

Substrate Reagent Pack
By Anonymous on 06/15/2018

Substrate Reagent Pack
By Anonymous on 05/10/2018

Substrate Reagent Pack
By Leslie Priddy on 03/30/2018

Substrate Reagent Pack
By Anonymous on 02/22/2018

Substrate Reagent Pack
By Anonymous on 02/06/2018

Substrate Reagent Pack
By Anonymous on 02/06/2018

Substrate Reagent Pack
By Anonymous on 01/19/2018

Substrate Reagent Pack
By Balaji Mahender on 12/20/2017

Substrate Reagent Pack
By Jomkuan Theprungsirikul on 12/15/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Jomkuan Theprungsirikul on 12/05/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By David Bolick on 09/07/2017

Our lab does many ELISAs. These substrate solutions are excellent, affordable, and easy to use.


Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/19/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/17/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/14/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/06/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Goutham Pattabiraman on 06/15/2017

Really Easy to use.
After the SA-HRP detection step for ELISA, wash wells 4 times with wash buffer, and add the Substrate and incubate for 20 mins in the dark. Stop the reaction using 0.2 M sulfuric acid. Read the plate at 450nm


Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Abby Sukarto on 05/03/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 03/29/2017

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 11/28/2016

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Adam Guess on 09/01/2016

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By John Yu on 04/26/2016

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 01/18/2016