Substrate Reagent Pack

8 vials Color A, 8 vials Color B
Catalog # Availability Size / Price Qty
DY999
Ancillary Products Available
Substrate Reagent Pack
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Product Details
Citations (82)
FAQs
Supplemental Products
Reviews (37)

Substrate Reagent Pack Summary

INTENDED USE & DESCRIPTION

Substrate Reagent Pack contains 8 vials of Color Reagent A (stabilized hydrogen peroxide, Part # 895000) and 8 vials of Color Reagent B (stabilized tetramethylbenzidine, Part # 895001), both at 12 mL/vial, producing color development when contacting HRP-conjugated or biotinylated, streptavidin-HRP bound detection antibodies.   

STORAGE & STABILITY

Store unopened at 2-8 °C. Stable for up to 30 days after opening when stored at 2-8 °C.

REAGENT PREPARATION

Warm to room temperature before use. Color Reagent A and Color Reagent B should be mixed together in equal volumes within 15 minutes of use. Protect from light. Add 100 µL of the mixture to each well and incubate at room temperature for 20 minutes while protecting from light. Add Stop Solution (Catalog # DY994) and read the microplate.

TECHNICAL HINTS & LIMITATIONS OF THE PROCEDURE 

  • Caution: Color Reagent B may cause skin, eye, and respiratory irritation. Avoid breathing fumes. Wear protective gloves, clothing, and eye and face protection. Wash hands thoroughly after handling. Please refer to the MSDS on our website prior to use. 

Specifications

Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Applications
ELISA

Product Datasheets

Data Example

Substrate Reagent Pack

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Reagents Provided

Other Reagents Provided

Citations for Substrate Reagent Pack

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

82 Citations: Showing 1 - 10
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  1. Utility of Centrifugation-Controlled Convective (C3) Flow for Rapid On-chip ELISA
    Authors: W Espulgar, T Tadokoro, E Tamiya, M Saito
    Sci Rep, 2019;9(1):20150.  2019
  2. Infliximab Induced a Dissociated Response of Severe Periodontal Biomarkers in Rheumatoid Arthritis Patients
    Authors: M Rinaudo-Ga, V Blasco-Baq, P Miossec, P Gaudin, P Farge, X Roblin, T Thomas, S Paul, H Marotte
    J Clin Med, 2019;8(5):.  2019
  3. Natural Killer Response and Lipo-Metabolic Profile in Adults with Low HDL-Cholesterol and Mild Hypercholesterolemia: Beneficial Effects of Artichoke Leaf Extract Supplementation
    Authors: M Rondanelli, AM Castellazz, A Riva, P Allegrini, MA Faliva, G Peroni, M Naso, M Nichetti, C Tagliacarn, C Valsecchi, T Fazia, S Perna, F Graziano, M Grassi, L Bernardine
    Evid Based Complement Alternat Med, 2019;2019(0):2069701.  2019
  4. Electrostatic complex of neurotrophin 4 with dendrimer nanoparticles: controlled release of protein in vitro and in vivo
    Authors: M D?bkowska, D Rogi?ska, P K?os, A Sobu?, M Adamczak, Z Litwi?ska, A Machali?sk, B Machali?sk
    Int J Nanomedicine, 2019;14(0):6117-6131.  2019
  5. IL12 p35 and p40 subunit genes administered as pPAL plasmid constructs do not improve protection of pPAL-LACK vaccine against canine leishmaniasis
    Authors: PJ Alcolea, A Alonso, A Esteban, P Peris, A Cortés, JA Castillo, V Larraga
    PLoS ONE, 2019;14(2):e0212136.  2019
  6. Nkx-2.5 Regulates MDR1 Expression via Its Upstream Promoter in Breast Cancer Cells
    Authors: JS Lim, GY Jung, SY Park
    J. Korean Med. Sci., 2019;34(12):e100.  2019
  7. Increased expression of fibronectin leucine-rich transmembrane protein 3 in the dorsal root ganglion induces neuropathic pain in rats
    Authors: M Yamada, Y Fujita, Y Hayano, H Hayakawa, K Baba, H Mochizuki, T Yamashita
    J. Neurosci., 2019;0(0):.  2019
  8. Circulating Lactonase Activity but Not Protein Level of PON-1 Predicts Adverse Outcomes in Subjects with Chronic Kidney Disease
    Authors: CJ Mohammed, Y Xie, PS Brewster, S Ghosh, P Dube, T Sarsour, AL Kleinhenz, EL Crawford, D Malhotra, RW James, PA Kalra, ST Haller, DJ Kennedy
    J Clin Med, 2019;8(7):.  2019
  9. GPR31-dependent dendrite protrusion of intestinal CX3CR1+ cells by bacterial metabolites
    Authors: N Morita, E Umemoto, S Fujita, A Hayashi, J Kikuta, I Kimura, T Haneda, T Imai, A Inoue, H Mimuro, Y Maeda, H Kayama, R Okumura, J Aoki, N Okada, T Kida, M Ishii, R Nabeshima, K Takeda
    Nature, 2019;0(0):.  2019
  10. SRPK1 maintains acute myeloid leukemia through effects on isoform usage of epigenetic regulators including BRD4
    Authors: K Tzelepis, E De Braekel, D Aspris, I Barbieri, MS Vijayabask, WH Liu, M Gozdecka, E Metzakopia, HD Toop, M Dudek, SC Robson, F Hermida-Pr, YH Yang, R Babaei-Jad, DA Garyfallos, H Ponstingl, JML Dias, P Gallipoli, M Seiler, S Buonamici, B Vick, AJ Bannister, R Rad, RK Prinjha, JC Marioni, B Huntly, J Batson, JC Morris, C Pina, A Bradley, I Jeremias, DO Bates, K Yusa, T Kouzarides, GS Vassiliou
    Nat Commun, 2018;9(1):5378.  2018
  11. GM1 ganglioside prevents axonal regeneration inhibition and cognitive deficits in a mouse model of traumatic brain injury
    Authors: A Benady, D Freidin, CG Pick, V Rubovitch
    Sci Rep, 2018;8(1):13340.  2018
  12. Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs
    Authors: CH An, S Nazki, SC Park, YJ Jeong, JH Lee, SJ Park, A Khatun, WI Kim, YI Park, JC Jeong, CY Kim
    Planta, 2018;0(0):.  2018
  13. H7 Hemagglutinin nanoparticles retain immunogenicity after >3 months of 25�C storage
    Authors: TZ Chang, L Deng, BZ Wang, JA Champion
    PLoS ONE, 2018;13(8):e0202300.  2018
  14. Soluble interleukin-27 receptor alpha is a valuable prognostic biomarker for acute graft-versus-host disease after allogeneic haematopoietic stem cell transplantation
    Authors: S Liu, J Han, H Gong, Y Li, X Bao, J Qi, H Liu, J Chen, X Wu, Y Xu, S Ma, D Wu
    Sci Rep, 2018;8(1):10328.  2018
  15. Inhibition of anthrax lethal factor by ssDNA aptamers
    Authors: M Lahousse, HC Park, SC Lee, NR Ha, IP Jung, SR Schlesinge, K Shackelfor, MY Yoon, SK Kim
    Arch. Biochem. Biophys., 2018;646(0):16-23.  2018
  16. Safety Studies of Pneumococcal Endolysins Cpl-1 and Pal
    Authors: M Harhala, DC Nelson, P Miernikiew, RD Heselpoth, B Brzezicka, J Majewska, SB Linden, X Shang, A Szymczak, D Lecion, K Marek-Buko, M K?ak, B Wojciechow, K Lahutta, A Konieczny, K D?browska
    Viruses, 2018;10(11):.  2018
  17. Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs
    Authors: J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney
    Adv Healthc Mater, 2018;0(0):.  2018
  18. Soluble CD14 as a Diagnostic Biomarker for Smear-Negative HIV-Associated Tuberculosis
    Authors: Y Liu, OC Ndumnego, T Chen, RS Kim, ER Jenny-Avit, T Ndung'u, D Wilson, JM Achkar
    Pathogens, 2018;7(1):.  2018
  19. Serum Soluble Vascular Cell Adhesion Molecule-1 Overexpression Is a Disease Marker in Patients with First-Time Diagnosed Antinuclear Antibodies: A Prospective, Observational Pilot Study
    Authors: M Oleszowsky, MF Seidel
    Biomed Res Int, 2018;2018(0):8286067.  2018
  20. Elevated Hsp90-beta contributes to differential diagnosis of pleural effusion caused by lung cancer and correlates with malignant biological behavior of lung cancer
    Authors: R Biaoxue, L Min, F Tian, G Wenlong, L Hua
    BMC Pulm Med, 2018;18(1):188.  2018
  21. Neuroprotection by Caffeine in Hyperoxia-Induced Neonatal Brain Injury
    Authors: S Endesfelde, U Weichelt, E Strauá, A Schl”r, M Sifringer, T Scheuer, C Bhrer, T Schmitz
    Int J Mol Sci, 2017;18(1):.  2017
  22. Evaluation of multivalent H2 influenza pandemic vaccines in mice
    Authors: BJ Lenny, S Sonnberg, AF Danner, K Friedman, RJ Webby, RG Webster, JC Jones
    Vaccine, 2017;0(0):.  2017
  23. Lettuce-produced hepatitis C virus E1E2 heterodimer triggers immune responses in mice and antibody production after oral vaccination
    Authors: J Liu Clarke, L Paruch, MO Dobrica, I Caras, C Tucureanu, A Onu, S Ciulean, C Stavaru, A Eerde, Y Wang, H Steen, S Haugslien, C Petrareanu, C Lazar, I Popescu, R Bock, J Dubuisson, N Branza-Nic
    Plant Biotechnol. J., 2017;0(0):.  2017
  24. Restoring GM1 ganglioside expression ameliorates axonal outgrowth inhibition and cognitive impairments induced by blast traumatic brain injury
    Authors: V Rubovitch, Y Zilberstei, J Chapman, S Schreiber, CG Pick
    Sci Rep, 2017;7(0):41269.  2017
  25. Demyelination induced by oxidative stress is regulated by sphingosine 1-phosphate receptors
    Authors: SA O'Sullivan, M Velasco-Es, KK Dev
    Glia, 2017;0(0):.  2017
  26. Vitronectin as a Micromanager of Cell Response in Material-Driven Fibronectin Nanonetworks
    Authors: M Cantini, K Gomide, V Moulisova, C González-G, M Salmerón-S
    Adv Biosyst, 2017;1(9):1700047.  2017
  27. Antigen-specific immunoglobulin variable region sequencing measures humoral immune response to vaccination in the equine neonate
    Authors: RL Tallmadge, SC Miller, SA Parry, MJB Felippe
    PLoS ONE, 2017;12(5):e0177831.  2017
  28. Extracellular Lactate Dehydrogenase A Release From Damaged Neurons Drives Central Nervous System Angiogenesis
    Authors: H Lin, R Muramatsu, N Maedera, H Tsunematsu, M Hamaguchi, Y Koyama, M Kuroda, K Ono, M Sawada, T Yamashita
    EBioMedicine, 2017;0(0):.  2017
  29. IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice
    Authors: TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray
    JCI Insight, 2017;2(10):.  2017
  30. YeeJ is an inverse autotransporter from Escherichia coli that binds to peptidoglycan and promotes biofilm formation
    Authors: M Martinez-G, KGK Goh, E Rackaityte, C Sakamoto, B Audrain, DG Moriel, M Totsika, JM Ghigo, MA Schembri, C Beloin
    Sci Rep, 2017;7(1):11326.  2017
  31. The ESRP1-GPR137 axis contributes to intestinal pathogenesis
    Authors: LF Mager, VH Koelzer, R Stuber, L Thoo, I Keller, I Koeck, M Langenegge, C Simillion, SP Pfister, M Faderl, V Genitsch, I Tcymbarevi, P Juillerat, X Li, Y Xia, E Karamitopo, R Lyck, I Zlobec, S Hapfelmeie, R Bruggmann, KD McCoy, AJ Macpherson, C Müller, B Beutler, P Krebs
    Elife, 2017;6(0):.  2017
  32. Marked elevation in plasma osteoprotegerin constitutes an early and consistent feature of cerebral malaria
    Thromb. Haemost., 2016;115(4):773-80.  2016
  33. The Robo4 cytoplasmic domain is dispensable for vascular permeability and neovascularization
    Nat Commun, 2016;7(0):13517.  2016
  34. Regulation of C3 Activation by the Alternative Complement Pathway in the Mouse Retina
    PLoS ONE, 2016;11(8):e0161898.  2016
  35. Non-Human Primates Harbor Diverse Mammalian and Avian Astroviruses Including Those Associated with Human Infections.
    Authors: Karlsson E, Small C, Freiden P, Feeroz M, Matsen F, San S, Hasan M, Wang D, Jones-Engel L, Schultz-Cherry S
    PLoS Pathog, 2015;11(11):e1005225.  2015
  36. Innate immunity drives the initiation of a murine model of primary biliary cirrhosis.
    Authors: Chang C, Chen Y, Zhang W, Leung P, Gershwin M, Chuang Y
    PLoS ONE, 2015;10(3):e0121320.  2015
  37. A C-terminal HSP90 inhibitor restores glucocorticoid sensitivity and relieves a mouse allograft model of Cushing disease.
    Authors: Riebold M, Kozany C, Freiburger L, Sattler M, Buchfelder M, Hausch F, Stalla G, Paez-Pereda M
    Nat Med, 2015;21(3):276-80.  2015
  38. Mechanism of the antigen-independent cytokinergic SPE-7 IgE activation of human mast cells in vitro.
    Authors: Bax H, Bowen H, Dodev T, Sutton B, Gould H
    Sci Rep, 2015;5(0):9538.  2015
  39. Bone marrow-derived macrophages from aged rats are more responsive to inflammatory stimuli.
    Authors: Barrett J, Costello D, O'Sullivan J, Cowley T, Lynch M
    J Neuroinflammation, 2015;12(0):67.  2015
  40. Macrophage Migration Inhibitory Factor in Acute Adipose Tissue Inflammation.
    Authors: Kim B, Rongisch R, Hager S, Grieb G, Nourbakhsh M, Rennekampff H, Bucala R, Bernhagen J, Pallua N
    PLoS ONE, 2015;10(9):e0137366.  2015
  41. Interleukin-22 is increased in multiple sclerosis patients and targets astrocytes.
    Authors: Perriard G, Mathias A, Enz L, Canales M, Schluep M, Gentner M, Schaeren-Wiemers N, Du Pasquier R
    J Neuroinflammation, 2015;12(0):119.  2015
  42. Crucial role of IL1beta and C3a in the in vitro-response of multipotent mesenchymal stromal cells to inflammatory mediators of polytrauma.
    Authors: Hengartner N, Fiedler J, Schrezenmeier H, Huber-Lang M, Brenner R
    PLoS ONE, 2015;10(1):e0116772.  2015
  43. An automated method for determining the cytoadhesion of Plasmodium falciparum-infected erythrocytes to immobilized cells.
    Authors: Hempel C, Boisen I, Efunshile A, Kurtzhals J, Staalso T
    Malar J, 2015;14(0):112.  2015
  44. Upregulated interleukin-21 receptor on B cells associated with the downregulation of IgE in patients with allergic rhinitis.
    Authors: Jen, Hsiao-Yu, Yang, Yao-Hsu, Chiang, Bor-Luen, Chuang, Ya-Hui
    J Interferon Cytokine Res, 2015;35(1):42-8.  2015
  45. Early decline in serum phospho-CSE1L levels in vemurafenib/sunitinib-treated melanoma and sorafenib/lapatinib-treated colorectal tumor xenografts.
    Authors: Lee W, Shen S, Shih Y, Chou C, Tseng J, Chin S, Liu K, Chen Y, Jiang M
    J Transl Med, 2015;13(0):191.  2015
  46. Alloimmune activation promotes anti-cancer cytotoxicity after rat liver transplantation.
    Authors: Lacotte S, Oldani G, Slits F, Orci L, Rubbia-Brandt L, Morel P, Mentha G, Toso C
    PLoS ONE, 2014;9(3):e91515.  2014
  47. Cyclooxygenase inhibition abrogates aeroallergen-induced immune tolerance by suppressing prostaglandin I2 receptor signaling.
    Authors: Zhou W, Goleniewska K, Zhang J, Dulek D, Toki S, Lotz M, Newcomb D, Boswell M, Polosukhin V, Milne G, Wu P, Moore M, FitzGerald G, Peebles R
    J Allergy Clin Immunol, 2014;134(3):698-705.e5.  2014
  48. Nonfunctional variant 3 factor H binding proteins as meningococcal vaccine candidates.
    Authors: van der Veen, Stijn, Johnson, Steven, Jongerius, Ilse, Malik, Talat, Genovese, Alessia, Santini, Laura, Staunton, David, Ufret-Vincenty, Rafael L, Pickering, Matthew, Lea, Susan M, Tang, Christop
    Infect Immun, 2014;82(3):1157-63.  2014
  49. GPR56 inhibits melanoma growth by internalizing and degrading its ligand TG2.
    Authors: Yang L, Friedland S, Corson N, Xu L
    Cancer Res, 2014;74(4):1022-31.  2014
  50. Modulation of endotoxicity of Shigella generalized modules for membrane antigens (GMMA) by genetic lipid A modifications: relative activation of TLR4 and TLR2 pathways in different mutants.
    Authors: Rossi O, Pesce I, Giannelli C, Aprea S, Caboni M, Citiulo F, Valentini S, Ferlenghi I, MacLennan C, D'Oro U, Saul A, Gerke C
    J Biol Chem, 2014;289(36):24922-35.  2014
  51. Hepatocytes induce Foxp3(+) regulatory T cells by Notch signaling.
    Authors: Burghardt S, Claass B, Erhardt A, Karimi K, Tiegs G
    J Leukoc Biol, 2014;96(4):571-7.  2014
  52. Dimerization is not a determining factor for functional high affinity human plasminogen binding by the group A streptococcal virulence factor PAM and is mediated by specific residues within the PAM a1a2 domain.
    Authors: Bhattacharya S, Liang Z, Quek A, Ploplis V, Law R, Castellino F
    J Biol Chem, 2014;289(31):21684-93.  2014
  53. Microparticle alpha-2-macroglobulin enhances pro-resolving responses and promotes survival in sepsis.
    Authors: Dalli, Jesmond, Norling, Lucy V, Montero-Melendez, Trinidad, Federici Canova, Donata, Lashin, Hazem, Pavlov, Anton M, Sukhorukov, Gleb B, Hinds, Charles, Perretti, Mauro
    EMBO Mol Med, 2014;6(1):27-42.  2014
  54. Netrin-1 controls sympathetic arterial innervation.
    Authors: Brunet I, Gordon E, Han J, Cristofaro B, Broqueres-You D, Liu C, Bouvree K, Zhang J, Del Toro R, Mathivet T, Larrivee B, Jagu J, Pibouin-Fragner L, Pardanaud L, Machado M, Kennedy T, Zhuang Z, Simons M, Levy B, Tessier-Lavigne M, Grenz A, Eltzschig H, Eichmann A
    J Clin Invest, 2014;124(7):3230-40.  2014
  55. Cationic lipid-formulated DNA vaccine against hepatitis B virus: immunogenicity of MIDGE-Th1 vectors encoding small and large surface antigen in comparison to a licensed protein vaccine.
    Authors: Endmann A, Klunder K, Kapp K, Riede O, Oswald D, Talman E, Schroff M, Kleuss C, Ruiters M, Juhls C
    PLoS ONE, 2014;9(7):e101715.  2014
  56. Detection of antibodies against Turkey astrovirus in humans.
    Authors: Meliopoulos V, Kayali G, Burnham A, Oshansky C, Thomas P, Gray G, Beck M, Schultz-Cherry S
    PLoS ONE, 2014;9(5):e96934.  2014
  57. Shedding of c-Met ectodomain correlates with c-Met expression in non-small cell lung cancer.
    Authors: Fu, Le, Guo, Wei, Liu, Bingshan, Sun, Linlin, Bi, Zhenghon, Zhu, Li, Wang, Xinyan, Liu, Bin, Xie, Qian, Li, Ke
    Biomarkers, 2013;18(2):126-35.  2013
  58. Latent TGF-beta binding protein 4 promotes elastic fiber assembly by interacting with fibulin-5.
    Authors: Noda K, Dabovic B, Takagi K, Inoue T, Horiguchi M, Hirai M, Fujikawa Y, Akama T, Kusumoto K, Zilberberg L, Sakai L, Koli K, Naitoh M, von Melchner H, Suzuki S, Rifkin D, Nakamura T
    Proc Natl Acad Sci U S A, 2013;110(8):2852-7.  2013
  59. The CD8-derived chemokine XCL1/lymphotactin is a conformation-dependent, broad-spectrum inhibitor of HIV-1.
    Authors: Guzzo C, Fox J, Lin Y, Miao H, Cimbro R, Volkman B, Fauci A, Lusso P
    PLoS Pathog, 2013;9(12):e1003852.  2013
  60. A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys.
    Authors: Ohshima-Hosoyama S, Simmons HA, Goecks N
    PLoS ONE, 2012;7(6):e39036.  2012
  61. A novel role for insulin resistance in the connection between obesity and postmenopausal breast cancer.
    Authors: Weichhaus M, Broom J, Wahle K, Bermano G
    Int. J. Oncol., 2012;41(2):745-52.  2012
  62. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012;7(2):e32125.  2012
  63. Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide.
    Authors: Ibebuogu UN, Gladysheva IP, Houng AK
    Circ Heart Fail, 2011;4(2):114-20.  2011
  64. Influence of galectin-9/Tim-3 interaction on herpes simplex virus-1 latency.
    Authors: Reddy PB, Sehrawat S, Suryawanshi A
    J. Immunol., 2011;187(11):5745-55.  2011
  65. Apolipoprotein B binding domains: evidence that they are cell-penetrating peptides that efficiently deliver antigenic peptide for cross-presentation of cytotoxic T cells.
    Authors: Sakamoto N, Rosenberg AS,
    J. Immunol., 2011;186(8):5004-11.  2011
  66. Adiponectin inhibits leptin signalling via multiple mechanisms to exert protective effects against hepatic fibrosis.
    Authors: Handy JA, Fu PP, Kumar P
    Biochem. J., 2011;440(3):385-95.  2011
  67. Combined Flt3L/TK gene therapy induces immunological surveillance which mediates an immune response against a surrogate brain tumor neoantigen.
    Authors: King GD, Muhammad AK, Larocque D
    Mol. Ther., 2011;19(10):1793-801.  2011
  68. A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.
    Authors: Kotarsky K, Sitnik KM, Stenstad H, Kotarsky H, Schmidtchen A, Koslowski M, Wehkamp J, Agace WW
    Mucosal Immunol, 2010;3(1):40-8.  2010
  69. Enzyme-linked enzyme-binding assay for Pin1 WW domain ligands.
    Authors: Mercedes-Camacho AY, Etzkorn FA,
    Anal. Biochem., 2010;402(1):77-82.  2010
  70. Macrophage migration inhibitory factor anti-thrombin III complexes are decreased in bladder cancer patient serum: Complex formation as a mechanism of inactivation.
    Authors: Meyer-Siegler KL, Cox J, Leng L, Bucala R, Vera PL
    Cancer Lett., 2010;290(1):49-57.  2010
  71. Evaluation of soluble junctional adhesion molecule-A as a biomarker of human brain endothelial barrier breakdown.
    Authors: Haarmann A, Deiss A, Prochaska J, Foerch C, Weksler B, Romero I, Couraud PO, Stoll G, Rieckmann P, Buttmann M
    PLoS ONE, 2010;5(10):e13568.  2010
  72. Systemic immunization with CCL27/CTACK modulates immune responses at mucosal sites in mice and macaques.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Khan AS, Draghia-Akli R, Sardesal NY, Lewis MG, Yan J, Weiner DB
    Vaccine, 2010;28(8):1942-51.  2010
  73. PEGylation of interleukin-10 for the mitigation of enhanced pain states.
    Authors: Soderquist RG, Milligan ED, Harrison JA, Chavez RA, Johnson KW, Watkins LR, Mahoney MJ
    J Biomed Mater Res A, 2010;93(3):1169-79.  2010
  74. Endothelin-converting enzyme-2 is increased in Alzheimer's disease and up-regulated by Abeta.
    Authors: Palmer JC, Baig S, Kehoe PG, Love S
    Am. J. Pathol., 2009;175(1):262-70.  2009
  75. A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
    Authors: Tatsumi R, Wuollet AL, Tabata K, Nishimura S, Tabata S, Mizunoya W, Ikeuchi Y, Allen RE
    Am. J. Physiol., Cell Physiol., 2009;296(4):C922-9.  2009
  76. Nanobacteria are mineralo fetuin complexes.
    Authors: Raoult D, Drancourt M, Azza S, Nappez C, Guieu R, Rolain JM, Fourquet P, Campagna B, La Scola B, Mege JL, Mansuelle P, Lechevalier E, Berland Y, Gorvel JP, Renesto P
    PLoS Pathog., 2008;4(2):e41.  2008
  77. Up-regulated macrophage migration inhibitory factor protects apoptosis of dermal fibroblasts in patients with systemic sclerosis.
    Authors: Kim JY, Kwok SK, Hur KH, Kim HJ, Kim NS, Yoo SA, Kim WU, Cho CS
    Clin. Exp. Immunol., 2008;152(2):328-35.  2008
  78. Soluble ErbB3 levels in bone marrow and plasma of men with prostate cancer.
    Authors: Lin SH, Lee YC, Choueiri MB, Wen S, Mathew P, Ye X, Do KA, Navone NM, Kim J, Tu SM, Yu-Lee LY, Logothetis CJ
    Clin. Cancer Res., 2008;14(12):3729-36.  2008
  79. Soluble triggering receptor expressed on myeloid cell-1 is increased in patients with ventilator-associated pneumonia: a preliminary report.
    Authors: Horonenko G, Hoyt JC, Robbins RA, Singarajah CU, Umar A, Pattengill J, Hayden JM
    Chest, 2007;132(1):58-63.  2007
  80. Human cytomegalovirus-specific CD4+-T-cell cytokine response induces fractalkine in endothelial cells.
    Authors: Bolovan-Fritts CA, Trout RN, Spector SA
    J. Virol., 2004;78(23):13173-81.  2004
  81. ELR+ CXC chemokines in human milk.
    Authors: Maheshwari A, Christensen RD, Calhoun DA
    Cytokine, 2003;24(3):91-102.  2003
  82. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 0;156(1):74-85.  0

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Substrate Reagent Pack
By Anonymous on 02/11/2020
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Substrate Reagent Pack
By Anonymous on 01/17/2020
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Substrate Reagent Pack
By Anonymous on 01/10/2020
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Substrate Reagent Pack
By Anonymous on 12/23/2019
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One pair of A and B is enough for one plate which makes it very easy to use while keep the reagent stable


Substrate Reagent Pack
By Anonymous on 09/07/2019
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Substrate Reagent Pack
By Anonymous on 07/02/2019
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Substrate Reagent Pack
By Anonymous on 03/28/2019
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I used this a my detection substrate (100uL) in a direct ELISA after my plate was treated with an anti-mouse IgG-HRP antibody. I find the background is very low which allows me to achieved a better sensitivity with my ELISAs compared another commercial substrate


Substrate Reagent Pack
By Anonymous on 03/13/2019
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Substrate Reagent Pack
By Anonymous on 01/03/2019
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Substrate Reagent Pack
By Anonymous on 12/10/2018
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Substrate Reagent Pack
By Anonymous on 11/21/2018
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It is very stable before mixing the reagent A and B. One bottle of each is exactly enough for two plates which makes measuring simple.


Substrate Reagent Pack
By Anonymous on 11/13/2018
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Substrate Reagent Pack
By Anonymous on 09/30/2018
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Substrate Reagent Pack
By Anonymous on 08/17/2018
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Substrate Reagent Pack
By Anonymous on 06/19/2018
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Substrate Reagent Pack
By Anonymous on 06/15/2018
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Substrate Reagent Pack
By Anonymous on 05/10/2018
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Substrate Reagent Pack
By Anonymous on 03/30/2018
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Substrate Reagent Pack
By Anonymous on 02/22/2018
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Substrate Reagent Pack
By Anonymous on 02/06/2018
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Substrate Reagent Pack
By Anonymous on 02/06/2018
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Substrate Reagent Pack
By Anonymous on 01/19/2018
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Substrate Reagent Pack
By Anonymous on 12/20/2017
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Substrate Reagent Pack
By Anonymous on 12/15/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 12/05/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 09/07/2017
Application:

Our lab does many ELISAs. These substrate solutions are excellent, affordable, and easy to use.


Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/19/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/17/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/14/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 07/06/2017
Application:

Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 06/15/2017
Application:

Really Easy to use.
After the SA-HRP detection step for ELISA, wash wells 4 times with wash buffer, and add the Substrate and incubate for 20 mins in the dark. Stop the reaction using 0.2 M sulfuric acid. Read the plate at 450nm


Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 05/03/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 03/29/2017
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 11/28/2016
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 09/01/2016
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 04/26/2016
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Substrate Reagent Pack (8 vials Color A, 8 vials Color B)
By Anonymous on 01/18/2016
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