Human/Mouse/Bovine Insulin Alexa Fluor® 488-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry View Larger

Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC1417G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Preparation and Storage

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Product Datasheets

Related Research Areas

• Diabetes
• Endocrine Regulation of Lipid Metabolism
• Glucose Homeostasis
• Growth Hormone/IGF-I Axis
• IGF Family
• Metabolic Peptide Hormones and Regulators
• Metabolism
• Nutrition and Appetite
• Pancreatic Endoderm
• Pancreatic Endoderm Cell Markers
• Preeclampsia