Human/Mouse/Bovine Insulin APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC1417A
Detection of Insulin in  beta TC‑6 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human/Mouse/Bovine Insulin APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects bovine, human, and mouse insulin.
Source
Monoclonal Rat IgG2A Clone # 182410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Insulin
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Insulin in  beta TC‑6 Mouse Cell Line by Flow Cytometry. View Larger

Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC‑conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

Product Datasheets

Citations for Human/Mouse/Bovine Insulin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes
    Authors: J Dooley, L Tian, S Schonefeld, V Delghingar, JE Garcia-Per, E Pasciuto, D Di Marino, EJ Carr, N Oskolkov, V Lyssenko, D Franckaert, V Lagou, L Overbergh, J Vandenbuss, J Allemeersc, G Chabot-Roy, JE Dahlstrom, DR Laybutt, N Petrovsky, L Socha, K Gevaert, AM Jetten, D Lambrechts, MA Linterman, CC Goodnow, CJ Nolan, S Lesage, SM Schlenner, A Liston
    Nat. Genet., 2016;48(5):519-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow
  2. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013;46(4):259-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow

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