Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC‑conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.
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Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes Authors: J Dooley, L Tian, S Schonefeld, V Delghingar, JE Garcia-Per, E Pasciuto, D Di Marino, EJ Carr, N Oskolkov, V Lyssenko, D Franckaert, V Lagou, L Overbergh, J Vandenbuss, J Allemeersc, G Chabot-Roy, JE Dahlstrom, DR Laybutt, N Petrovsky, L Socha, K Gevaert, AM Jetten, D Lambrechts, MA Linterman, CC Goodnow, CJ Nolan, S Lesage, SM Schlenner, A Liston Nat. Genet., 2016;48(5):519-27. Species: Mouse Sample Type: Whole Cells Application: Flow
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