Human/Mouse/Bovine Insulin APC-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of Insulin in  beta TC‑6 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (7)
Supplemental Products

Human/Mouse/Bovine Insulin APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Detects bovine, human, and mouse insulin.
Monoclonal Rat IgG2A Clone # 182410
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Insulin
Supplied in a saline solution containing BSA and Sodium Azide.
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of Insulin antibody in  beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Insulin in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Rat Anti-Human/Mouse/Bovine Insulin APC-conjugated Monoclonal Antibody (Catalog # IC1417A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Insulin

Insulin is a disulfide-linked heterodimeric protein secreted by the pancreatic Islets of Langerhans. Mature insulin is generated by the proteolytic removal of a peptide from proinsulin. It is involved in the regulation of glucose metabolism through interactions with the Insulin Receptor.

Entrez Gene IDs
3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)
Alternate Names
IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

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Citations for Human/Mouse/Bovine Insulin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Differentially expressed microRNAs during the differentiation of muscle-derived stem cells into insulin-producing cells, a promoting role of microRNA-708-5p/STK4 axis
    Authors: Y Ren, X Wang, H Liang, Y Ma
    PLoS ONE, 2022;17(4):e0266609.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. The IGFBP3/TMEM219 pathway regulates beta cell homeostasis
    Authors: F D'Addio, A Maestroni, E Assi, M Ben Nasr, G Amabile, V Usuelli, C Loretelli, F Bertuzzi, B Antonioli, F Cardarelli, B El Essawy, A Solini, IC Gerling, C Bianchi, G Becchi, S Mazzucchel, D Corradi, GP Fadini, D Foschi, JF Markmann, E Orsi, J Škrha, MG Camboni, R Abdi, AM James Shap, F Folli, J Ludvigsson, S Del Prato, G Zuccotti, P Fiorina
    Nature Communications, 2022;13(1):684.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. RNA aptamers specific for transmembrane p24 trafficking protein 6 and Clusterin for the targeted delivery of imaging reagents and RNA therapeutics to human beta cells
    Authors: D Van Simaey, A De La Fuen, S Zilio, A Zoso, V Kuznetsova, O Alcazar, P Buchwald, A Grilli, J Caroli, S Bicciato, P Serafini
    Nature Communications, 2022;13(1):1815.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. microRNA-690 regulates induced pluripotent stem cells (iPSCs) differentiation into insulin-producing cells by targeting Sox9
    Authors: Y Xu, Y Huang, Y Guo, Y Xiong, S Zhu, L Xu, J Lu, X Li, J Wan, Y Lu, Z Wang
    Stem Cell Res Ther, 2019;10(1):59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress
    Authors: C Merriman, D Fu
    J. Biol. Chem., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Genetic predisposition for beta cell fragility underlies type 1 and type 2 diabetes
    Authors: J Dooley, L Tian, S Schonefeld, V Delghingar, JE Garcia-Per, E Pasciuto, D Di Marino, EJ Carr, N Oskolkov, V Lyssenko, D Franckaert, V Lagou, L Overbergh, J Vandenbuss, J Allemeersc, G Chabot-Roy, JE Dahlstrom, DR Laybutt, N Petrovsky, L Socha, K Gevaert, AM Jetten, D Lambrechts, MA Linterman, CC Goodnow, CJ Nolan, S Lesage, SM Schlenner, A Liston
    Nat. Genet., 2016;48(5):519-27.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013;46(4):259-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry


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