Human/Mouse/Bovine Integrin  alpha 6/CD49f Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB13501G Datasheet / COA / SDS
Clone GoH3 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
FAB13501G-100UG
Detection of Integrin  alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry.
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Human/Mouse/Bovine Integrin  alpha 6/CD49f Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.
Source
Monoclonal Rat IgG2A Clone # GoH3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse mammary tumor cells
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

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Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of Integrin  alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of Integrin alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB13501G) and Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A). Quadrant markers were set based on control antibody staining (Catalog # IC006G). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of Integrin  alpha 6/CD49f in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of Integrin alpha 6/CD49f in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB13501G, filled histogram) or isotype control antibody (Catalog # IC006G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 6/CD49f

The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits and recognizes laminin as a ligand.

Entrez Gene IDs
3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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