Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

R&D Systems | Catalog # FAB13501A

Clone GoH3 was used by HLDA to establish CD designation
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human, Mouse, Bovine

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Affinity Purification

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # GoH3
View all Integrin alpha 6/CD49f Primary Antibodies »

Product Specifications

Immunogen

Mouse mammary tumor cells

Specificity

Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

Detection of Integrin a6/CD49f antibody in Human Blood Lymphocytes antibody by Flow Cytometry.

Detection of Integrin alpha 6/CD49f in Human Blood Lymphocytes by Flow Cytometry.

Human peripheral blood lymphocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f APC-conjugated Monoclonal Antibody (Catalog # FAB13501A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Integrin a6/CD49f antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of Integrin alpha 6/CD49f in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes were stained with Rat Anti-Human/Mouse/Bovine Integrin a6/CD49f APC-conjugated Monoclonal Antibody (Catalog # FAB13501A) and Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P). Quadrant markers were set based on control antibody staining (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood lymphocytes and mouse splenocytes

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Integrin alpha 6/CD49f

Integrin alpha 6 (also VLA-6, CD49F and ITA6) is a 130-150 kDa member of the Integrin alpha chain family of molecules. Mature Integrin  alpha 6 is a 1107 amino acid (aa) type I transmembrane glycoprotein that contains a 1027 aa extracellular region and a 93 aa cytoplasmic tail. The ECD is cleaved to generate a disulfide-linked dimer that shows a 125 kDa heavy chain (aa 24-938), and a 30-31 kDa membrane-bound light chain (aa 942-1130). Integrin alpha 6 forms a nondisulfide-linked heterodimeric complex with both integrin beta 1 and beta 4. Each complex serves as a receptor for laminin.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Gene Symbol

ITGA6

Additional Integrin alpha 6/CD49f Products

Product Documents for Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

For research use only

Citations for Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody

Customer Reviews

There are currently no reviews for this product. Be the first to review Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody and earn rewards!

Have you used Human/Mouse/Bovine Integrin  alpha 6/CD49f APC‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies