Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

R&D Systems | Catalog # MAB13501

Clone GoH3 was used by HLDA to establish CD designation
R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human, Mouse, Porcine, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # GoH3
View all Integrin alpha 6/CD49f Primary Antibodies »

Product Specifications

Immunogen

Mouse mammary tumor cells

Specificity

Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037. Knapp, W.B. et al. Eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. Aumailley, M.R. et al. (1990) Exp. Cell Res. 188:55.

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunohistochemistry

Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037.

Reviewed Applications

Read 4 reviews rated 5 using MAB13501 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 6/CD49f

The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits and recognizes laminin as a ligand.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Gene Symbol

ITGA6

Additional Integrin alpha 6/CD49f Products

Product Documents for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

For research use only

Citations for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

Customer Reviews for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody (4)

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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic retina and retina
    Species: Human
    Verified Customer | Posted 03/01/2022
    Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody MAB13501
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 09/09/2021
    Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody MAB13501
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: U937 human histiocytic lymphoma cell line
    Species: Human
    Verified Customer | Posted 05/20/2019
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 09/30/2018

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