Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

Clone GoH3 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB13501
MAB13501-SP
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody Summary

Species Reactivity
Human, Mouse, Bovine
Specificity
Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.
Source
Monoclonal Rat IgG2A Clone # GoH3
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse mammary tumor cells
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells
Immunohistochemistry

Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037.

 
Blockade of Receptor-ligand Interaction
Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037. Knapp, W.B. et al. Eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. Aumailley, M.R. et al. (1990) Exp. Cell Res. 188:55.
 
CyTOF-reported
This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin alpha 6/CD49f

The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits and recognizes laminin as a ligand.

Entrez Gene IDs
3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)
Alternate Names
CD49 antigen-like family member F; CD49f antigen; CD49f; DKFZp686J01244; FLJ18737; Integrin alpha 6; integrin alpha-6; integrin alpha6B; integrin, alpha 6; ITGA6; ITGA6B; Platelet gpl; VLA-6

Product Datasheets

Citations for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Phenotypic Analysis of BrdU Label-Retaining Cells during the Maturation of Conducting Airway Epithelium in a Porcine Lung
    Authors: Y Jia, X You, N Ma, H Li, G Liu, Y Wang, J Xue, J Shi, J Wei, Y Li, J Yang, X Liu
    Stem Cells Int, 2019;2019(0):7043890.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling
    Authors: NJ Weng, C Cheung, P Talbot
    Stem Cell Res, 2018;33(0):233-246.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Antibody-based targeting of alternatively spliced tissue factor: a new approach to impede the primary growth and spread of pancreatic ductal adenocarcinoma
    Oncotarget, 2016;7(18):25264-75.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  4. RANK signaling blockade reduces breast cancer recurrence by inducing tumor cell differentiation
    Cancer Res, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Endothelial podosome rosettes regulate vascular branching in tumour angiogenesis.
    Authors: Seano G, Chiaverina G, Gagliardi P, di Blasio L, Puliafito A, Bouvard C, Sessa R, Tarone G, Sorokin L, Helley D, Jain R, Serini G, Bussolino F, Primo L
    Nat Cell Biol, 2014;16(10):931-41, 1-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  6. alpha6-Integrin is required for the adhesion and vasculogenic potential of hemangioma stem cells.
    Authors: Smadja D, Guerin C, Boscolo E, Bieche I, Mulliken J, Bischoff J
    Stem Cells, 2014;32(3):684-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. Neuroligin 1 induces blood vessel maturation by cooperating with the alpha6 integrin.
    Authors: Samarelli A, Riccitelli E, Bizzozero L, Silveira T, Seano G, Pergolizzi M, Vitagliano G, Cascone I, Carpentier G, Bottos A, Primo L, Bussolino F, Arese M
    J Biol Chem, 2014;289(28):19466-76.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Monoclonal antibodies to human laminin alpha4 chain globular domain inhibit tumor cell adhesion and migration on laminins 411 and 421, and binding of alpha6beta1 integrin and MCAM to alpha4-laminins.
    Authors: Ishikawa T, Wondimu Z, Oikawa Y, Ingerpuu S, Virtanen I, Patarroyo M
    Matrix Biol, 2014;36(0):5-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  9. A novel multistep mechanism for initial lymphangiogenesis in mouse embryos based on ultramicroscopy.
    Authors: Hagerling R, Pollmann C, Andreas M, Schmidt C, Nurmi H, Adams R, Alitalo K, Andresen V, Schulte-Merker S, Kiefer F
    EMBO J, 2013;32(5):629-44.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. CD98hc (SLC3A2) regulation of skin homeostasis wanes with age.
    Authors: Boulter E, Estrach S, Errante A, Pons C, Cailleteau L, Tissot F, Meneguzzi G, Feral C
    J Exp Med, 2013;210(1):173-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Dedifferentiated follicular granulosa cells derived from pig ovary can transdifferentiate into osteoblasts.
    Authors: Oki, Yoshinao, Ono, Hiromasa, Motohashi, Takeharu, Sugiura, Nobuki, Nobusue, Hiroyuki, Kano, Koichiro
    Biochem J, 2012;447(2):239-48.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  12. Transcriptional activation of p53 by Pitx1.
    Authors: Liu DX, Lobie PE
    Cell Death Differ., 2007;14(11):1893-907.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Integrin-mediated host cell invasion by type 1-piliated uropathogenic Escherichia coli.
    Authors: Eto D, Jones T, Sundsbak J, Mulvey M
    PLoS Pathog, 0;3(7):e100.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization

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Reviews for Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody

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Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
By Anonymous on 05/20/2019
Application: WB Sample Tested: U937 human histiocytic lymphoma cell line Species: Human

Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
By Anonymous on 09/30/2018
Application: Flow Sample Tested: Peritoneal macrophages Species: Mouse