Integrin alpha 6/CD49f Antibody (GoH3)

R&D Systems | Catalog # MAB13501

Clone GoH3 was used by HLDA to establish CD designation
R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Bovine

Cited:

Human, Mouse, Porcine, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-reported

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, Bioassay, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # GoH3
View all Integrin alpha 6/CD49f Primary Antibodies »

Product Specifications

Immunogen

Mouse mammary tumor cells

Specificity

Detects human, mouse, and bovine Integrin alpha 6/CD49f. Recognizes an epitope in the extracellular domain of the Integrin alpha 6 subunit.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Integrin alpha 6/CD49f Antibody (GoH3)

Detection of bnosnryjaefcltnlovp2.jpg by Immunocytochemistry/ Immunofluorescence

Detection of bnosnryjaefcltnlovp2.jpg by Immunocytochemistry/ Immunofluorescence

Effect of HSF1 down-regulation on the growth and ability to differentiate of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 expression in either unmodified (WT) cells and cells transduced with lentiviruses containing shRNAs: control (sh.CTRL) or targeting HSF1 (sh1.HSF1, sh2.HSF1). Actin (ACTB) was used as a protein loading control. The graphs below show the results of densitometric analyses (n = 3). (b) Proliferation rates (assessed with crystal violet staining) of MCF10A (n = 5) and MCF12A (n = 3) cell variants. (c) The number of Ki-67 positive cells in MCF10A cell variants (n = 3). (d) Senescence-associated beta -galactosidase activity (yielding blue color) analyzed in MCF10A cell variants (n = 3). Scale bar 50 μm. Morphology of MCF10A (e) MCF12A (g) cell variants cultured in 2D (upper panels) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel; scale bar 20 µm). Arrows point to representative acini with disruptive morphology, and arrowheads indicate representative acini with multi-acinar morphology. (f) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. Statistically significant differences between unmodified cells, cells with empty vector or cells overexpressing HSF1 are marked. (h) Morphology of spheroids formed by MCF12A cell variants was distinguished as normal, disruptive and multi-acinar, as described by Ref [20]. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of cbahzowni61yztxplzxs.jpg by Immunocytochemistry/ Immunofluorescence

Detection of cbahzowni61yztxplzxs.jpg by Immunocytochemistry/ Immunofluorescence

Effect of HSF1 down-regulation on the growth and ability to differentiate of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 expression in either unmodified (WT) cells and cells transduced with lentiviruses containing shRNAs: control (sh.CTRL) or targeting HSF1 (sh1.HSF1, sh2.HSF1). Actin (ACTB) was used as a protein loading control. The graphs below show the results of densitometric analyses (n = 3). (b) Proliferation rates (assessed with crystal violet staining) of MCF10A (n = 5) and MCF12A (n = 3) cell variants. (c) The number of Ki-67 positive cells in MCF10A cell variants (n = 3). (d) Senescence-associated beta -galactosidase activity (yielding blue color) analyzed in MCF10A cell variants (n = 3). Scale bar 50 μm. Morphology of MCF10A (e) MCF12A (g) cell variants cultured in 2D (upper panels) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel; scale bar 20 µm). Arrows point to representative acini with disruptive morphology, and arrowheads indicate representative acini with multi-acinar morphology. (f) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. Statistically significant differences between unmodified cells, cells with empty vector or cells overexpressing HSF1 are marked. (h) Morphology of spheroids formed by MCF12A cell variants was distinguished as normal, disruptive and multi-acinar, as described by Ref [20]. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of h0b3jom1ydnycg1hftz3.jpg by Flow Cytometry

Detection of h0b3jom1ydnycg1hftz3.jpg by Flow Cytometry

Mesenchymal and stem cell-like characteristics of MCF10A and MCF12A cell variants with altered HSF1 expression. (a) Subpopulations defined by expression of EPCAM and ITGA6 or (b) CD24 and CD44 using flow cytometry. The percentages of subpopulations shown in the bar graphs are the mean ± SEM of three independent experiments. Ovals point out cell populations, which are enriched when HSF1 expression levels are altered. * p < 0.05 (significance of differences). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of hbztgz90jr62iigxkbjk.jpg by Immunocytochemistry/ Immunofluorescence

Detection of hbztgz90jr62iigxkbjk.jpg by Immunocytochemistry/ Immunofluorescence

HSF1 levels affect the expression of EMT markers and phenotype of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 and EMT markers in cells with overexpression (MCF10A) or knockdown (MCF12A) of HSF1. WT, unmodified cells; pLVX or shCTRL, cells transduced with lentiviruses containing the empty vector or control shRNA; HSF1-pLVX or sh1.HSF1, cells transduced with lentiviruses containing the HSF1 cDNA or shRNA targeting HSF1. ACTB and HSPA8 were used as loading controls. Graphs show the results of densitometric analyses (n = 3). (b) Proliferation rates assessed through crystal violet staining in MCF10A cell variants (n = 5). (c) Morphology of MCF10A cell variants in 2D culture (subconfluent; upper panel) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel). (d) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. (e,f) Confocal images of spheroids grown in Matrigel for 14 or 21 days and stained for CDH1 and DNA (scale bar 20 µm and 50 µm, respectively). (g) Migratory capacity of MCF10A cell variants analyzed with Boyden chamber assay (n = 3). (h) Time of cell detachment from the plastic surface by trypsinization. Boxplots represent the median, upper and lower quartiles, maximum and minimum. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

Effect of HSF1 down-regulation on the growth and ability to differentiate of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 expression in either unmodified (WT) cells and cells transduced with lentiviruses containing shRNAs: control (sh.CTRL) or targeting HSF1 (sh1.HSF1, sh2.HSF1). Actin (ACTB) was used as a protein loading control. The graphs below show the results of densitometric analyses (n = 3). (b) Proliferation rates (assessed with crystal violet staining) of MCF10A (n = 5) and MCF12A (n = 3) cell variants. (c) The number of Ki-67 positive cells in MCF10A cell variants (n = 3). (d) Senescence-associated beta -galactosidase activity (yielding blue color) analyzed in MCF10A cell variants (n = 3). Scale bar 50 μm. Morphology of MCF10A (e) MCF12A (g) cell variants cultured in 2D (upper panels) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel; scale bar 20 µm). Arrows point to representative acini with disruptive morphology, and arrowheads indicate representative acini with multi-acinar morphology. (f) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. Statistically significant differences between unmodified cells, cells with empty vector or cells overexpressing HSF1 are marked. (h) Morphology of spheroids formed by MCF12A cell variants was distinguished as normal, disruptive and multi-acinar, as described by Ref [20]. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

Effect of HSF1 down-regulation on the growth and ability to differentiate of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 expression in either unmodified (WT) cells and cells transduced with lentiviruses containing shRNAs: control (sh.CTRL) or targeting HSF1 (sh1.HSF1, sh2.HSF1). Actin (ACTB) was used as a protein loading control. The graphs below show the results of densitometric analyses (n = 3). (b) Proliferation rates (assessed with crystal violet staining) of MCF10A (n = 5) and MCF12A (n = 3) cell variants. (c) The number of Ki-67 positive cells in MCF10A cell variants (n = 3). (d) Senescence-associated beta -galactosidase activity (yielding blue color) analyzed in MCF10A cell variants (n = 3). Scale bar 50 μm. Morphology of MCF10A (e) MCF12A (g) cell variants cultured in 2D (upper panels) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel; scale bar 20 µm). Arrows point to representative acini with disruptive morphology, and arrowheads indicate representative acini with multi-acinar morphology. (f) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. Statistically significant differences between unmodified cells, cells with empty vector or cells overexpressing HSF1 are marked. (h) Morphology of spheroids formed by MCF12A cell variants was distinguished as normal, disruptive and multi-acinar, as described by Ref [20]. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Integrin alpha 6/CD49f by Flow Cytometry

Detection of Integrin alpha 6/CD49f by Flow Cytometry

Mesenchymal and stem cell-like characteristics of MCF10A and MCF12A cell variants with altered HSF1 expression. (a) Subpopulations defined by expression of EPCAM and ITGA6 or (b) CD24 and CD44 using flow cytometry. The percentages of subpopulations shown in the bar graphs are the mean ± SEM of three independent experiments. Ovals point out cell populations, which are enriched when HSF1 expression levels are altered. * p < 0.05 (significance of differences). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

Detection of Integrin alpha 6/CD49f by Immunocytochemistry/ Immunofluorescence

HSF1 levels affect the expression of EMT markers and phenotype of MCF10A and MCF12A cells. (a) Western blot analysis of HSF1 and EMT markers in cells with overexpression (MCF10A) or knockdown (MCF12A) of HSF1. WT, unmodified cells; pLVX or shCTRL, cells transduced with lentiviruses containing the empty vector or control shRNA; HSF1-pLVX or sh1.HSF1, cells transduced with lentiviruses containing the HSF1 cDNA or shRNA targeting HSF1. ACTB and HSPA8 were used as loading controls. Graphs show the results of densitometric analyses (n = 3). (b) Proliferation rates assessed through crystal violet staining in MCF10A cell variants (n = 5). (c) Morphology of MCF10A cell variants in 2D culture (subconfluent; upper panel) and Matrigel (middle and bottom panels). Phase-contrast images (upper and middle panels; scale bar 100 µm) and confocal images of spheroids stained for ITGA6 and DNA (bottom panel). (d) The volume of spheroids formed in Matrigel by MCF10A cell variants. Boxplots represent the median, upper and lower quartiles, maximum and minimum. (e,f) Confocal images of spheroids grown in Matrigel for 14 or 21 days and stained for CDH1 and DNA (scale bar 20 µm and 50 µm, respectively). (g) Migratory capacity of MCF10A cell variants analyzed with Boyden chamber assay (n = 3). (h) Time of cell detachment from the plastic surface by trypsinization. Boxplots represent the median, upper and lower quartiles, maximum and minimum. *** p < 0.001, ** p < 0.01, * p < 0.05 (significance of differences). Original western blots are presented in File S1. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37894333), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Integrin alpha 6/CD49f Antibody (GoH3)

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037. Knapp, W.B. et al. Eds. (1989) Leukocyte Typing IV: White Cell Differentiation Antigens, Oxford University Press, New York. Aumailley, M.R. et al. (1990) Exp. Cell Res. 188:55.

CyTOF-reported

This clone has been commercially reported for use in CyTOF®. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells

Immunohistochemistry

Sonnenberg, A. et al. (1986) J. Histochem. Cytochem. 34:1037.

Reviewed Applications

Read 4 reviews rated 5 using MAB13501 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Integrin alpha 6/CD49f

The Integrin alpha 6 subunit, also known as CD49f and VLA-6 alpha subunit, forms a heterodimer with Integrin beta 1 (CD29) or beta 4 (CD104) subunits and recognizes laminin as a ligand.

Alternate Names

CD49f, ITGA6, Platelet gpl, VLA-6

Entrez Gene IDs

3655 (Human); 16403 (Mouse); 101925271 (Cynomolgus Monkey)

Gene Symbol

ITGA6

Additional Integrin alpha 6/CD49f Products

Product Documents for Integrin alpha 6/CD49f Antibody (GoH3)

Certificate of Analysis

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Product Specific Notices for Integrin alpha 6/CD49f Antibody (GoH3)

For research use only

Citations for Integrin alpha 6/CD49f Antibody (GoH3)

Customer Reviews for Integrin alpha 6/CD49f Antibody (GoH3) (4)

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Customer Images

Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic retina and retina
    Species: Human
    Verified Customer | Posted 03/01/2022
    Integrin alpha 6/CD49f Antibody (GoH3) MAB13501
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 09/09/2021
    Integrin alpha 6/CD49f Antibody (GoH3) MAB13501
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: U937 human histiocytic lymphoma cell line
    Species: Human
    Verified Customer | Posted 05/20/2019
  • Human/Mouse/Bovine Integrin  alpha 6/CD49f Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Peritoneal macrophages
    Species: Mouse
    Verified Customer | Posted 09/30/2018

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