Key Product Details

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 275407
View all RGM-A Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse RGM-A
Cys48-Gly421
Accession # Q6PCX7

Specificity

Detects mouse RGM-A in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross‑reactivity with recombinant human RGM-A and recombinant chicken RGM is observed. In Western blots, approximately 25% cross-reactivity with recombinant mouse RGM-C is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human/Mouse/Chicken RGM‑A Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Repulsive Guidance Molecule A/RGM-A (Catalog # 2459-RM)
Recombinant Mouse Repulsive Guidance Molecule A/RGM-A (Catalog # 2458-RG)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: RGM-A

RGM-A is the founding member of the RGM family of glycosylphosphatidylinositol (GPI)- anchored axon guidance proteins. It is a disulfide-linked dimer of the N‑terminal and C-terminal RGD-containing segments. RGM-A binds with high affinity to Neogenin.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Q6PCX7

Additional RGM-A Products

Product Documents for Human/Mouse/Chicken RGM‑A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Chicken RGM‑A Antibody

For research use only

Related Research Areas

BMP Family

Citations for Human/Mouse/Chicken RGM‑A Antibody

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FAQs

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