Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC835G-025
IC835G-100
Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
Source
Monoclonal Rabbit IgG Clone # 269518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # U26943
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or  treated with 1 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC835G, filled histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Entrez Gene IDs
836 (Human); 12367 (Mouse)
Alternate Names
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA

Product Datasheets

Citations for Human/Mouse Cleaved Caspase-3 Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. EMAPII Monoclonal Antibody Ameliorates Influenza A Virus-Induced Lung Injury
    Authors: H Lu, S Chelvanamb, C Poirier, J Saliba, KL March, M Clauss, NV Bogatcheva
    Mol. Ther., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chemotherapy-induced differential cell cycle arrest in B cell lymphomas affects their sensitivity to Wee1 inhibition
    Authors: X Wang, Z Chen, AK Mishra, A Silva, W Ren, Z Pan, JH Wang
    Haematologica, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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