Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

(26 citations)
(3 Reviews)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
  • Source
    Monoclonal Rabbit IgG Clone # 269518
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    KLH-conjugated human Caspase-3 synthetic peptide
    CRGTELDCGIETD
    Accession # U26943
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase‑3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Immunocytochemistry
Caspase‑3 in Jurkat Human Cell Line. Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3
Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.
  • Entrez Gene IDs:
    836 (Human); 12367 (Mouse)
  • Alternate Names:
    Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017;8(7):e2955.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  2. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
    Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
    Sci Rep, 2017;7(1):8225.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. The protective effects of Resveratrol against radiation-induced intestinal injury
    Authors: H Zhang, H Yan, X Zhou, H Wang, Y Yang, J Zhang, H Wang
    BMC Complement Altern Med, 2017;17(1):410.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  4. Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist
    Authors: K Sato, R Shirai, M Hontani, R Shinooka, A Hasegawa, T Kichise, T Yamashita, H Yoshizawa, R Watanabe, TA Matsuyama, H Ishibashi-, S Koba, Y Kobayashi, T Hirano, T Watanabe
    J Am Heart Assoc, 2017;6(4):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Resveratrol fuels HER2 and ER?-positive breast cancer behaving as proteasome inhibitor
    Authors: C Andreani, C Bartolacci, K Wijnant, R Crinelli, M Bianchi, M Magnani, A Hysi, M Iezzi, A Amici, C Marchini
    Aging (Albany NY), 2017;9(2):508-523.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  6. Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice
    Authors: X Wang, GX Xue, WC Liu, H Shu, M Wang, Y Sun, X Liu, YE Sun, CF Liu, J Liu, W Liu, X Jin
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. H-Ras and K-Ras oncoproteins induce different tumor spectra when driven by the same regulatory sequences
    Cancer Res., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  8. Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever
    PLoS Pathog, 2016;12(5):e1005656.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  9. Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
    Authors: Himmelein S, Lindemann A, Sinicina I, Strupp M, Brandt T, Hufner K
    J Virol, 2015;89(10):5747-50.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  10. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2015;34(29):3826-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Fresh
  11. Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
    Authors: Yuan, Dongqing, Xu, Yidan, Hang, Hui, Liu, Xiaoyi, Chen, Xi, Xie, Ping, Yuan, Songtao, Zhang, Weiwei, Lin, Xiaojun, Liu, Qinghuai
    PLoS ONE, 2014;9(6):e99219.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  12. Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever.
    Authors: Oestereich L, Rieger T, Neumann M, Bernreuther C, Lehmann M, Krasemann S, Wurr S, Emmerich P, de Lamballerie X, Olschlager S, Gunther S
    PLoS Negl Trop Dis, 2014;8(5):e2804.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  13. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
    Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
    Int J Cancer, 2014;134(10):2489-503.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  14. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  15. Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics.
    Authors: Hahn W, Kuzmicic J, Burrill J, Donoghue M, Foncea R, Jensen M, Lavandero S, Arriaga E, Bernlohr D
    Am J Physiol Endocrinol Metab, 2014;306(9):E1033-45.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  16. The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
    Authors: Diefenbacher M, Popov N, Blake S, Schulein-Volk C, Nye E, Spencer-Dene B, Jaenicke L, Eilers M, Behrens A
    J Clin Invest, 2014;124(8):3407-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  17. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013;4(0):e627.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  18. Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
    Authors: Janek T, Krasowska A, Radwanska A, Lukaszewicz M
    PLoS ONE, 2013;8(3):e57991.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  19. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
    Authors: Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F
    Cancer Res, 2012;72(13):3424-36.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Frozen
  20. The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure.
    Authors: Downs I, Liu J, Aw TY, Adegboyega PA, Ajuebor MN
    PLoS ONE, 2012;7(6):e38051.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  21. In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression.
    Authors: Chen RY, Chen HX, Lin JX
    J. Exp. Clin. Cancer Res., 2010;29(0):13.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  22. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010;48(6):1225-35.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  23. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010;15(4):463-73.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  24. Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
    Authors: Terayama R, Bando Y, Murakami K, Kato K, Kishibe M, Yoshida S
    Neuroscience, 2007;148(1):175-87.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  25. Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
    Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
    J. Cell. Physiol., 2007;210(2):417-27.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
  26. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
    Authors: Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
    J. Cell. Sci., 2006;119(0):4247-56.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 26 Citations
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Average Rating: 4.3 (Based on 3 reviews)

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Read what people are saying who have used Human/Mouse Cleaved Caspase-3 (Asp175) Antibody.

We have 3 reviews tested in 2 species: Human, Mouse.
We have 3 reviews tested in 3 applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence.

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Images Ratings Applications Species Reviewed By Date Details
  Very Good
 Flow Mouse Anonymous 09/27/2017
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Summary

ApplicationFlow Cytometry
Sample TestedLiver tissue
SpeciesMouse
Immunohistochemistry Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
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Very Good
 IHC Mouse Anonymous 09/07/2017
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Image Details
Immunohistochemistry Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Immunohistochemistry: Human/Mouse Cleaved Caspase-3 (Asp175) Antibody [MAB835]

Summary

ApplicationImmunohistochemistry
Sample TestedEmbryonic brain
SpeciesMouse
Immunocytochemistry/Immunofluorescence Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
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Excellent
 ICC/IF Human Anonymous 02/08/2017
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Image Details
Immunocytochemistry/Immunofluorescence Human/Mouse Cleaved Caspase-3 (Asp175) Antibody MAB835
Immunocytochemistry/Immunofluorescence: Human/Mouse Cleaved Caspase-3 (Asp175) Antibody [MAB835]

Summary

ApplicationImmunocytochemistry/Immunofluorescence
Sample TestedIPS2 induced pluripotent stem cells derived cells
SpeciesHuman

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