Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Rat, Porcine, Transgenic Mouse, Xenograft

Applications

Validated:

Immunohistochemistry, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunocytochemistry/ Immunofluorescence, Dot Blot, IHC Paraffin-embedded, Westen Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 269518
View all Caspase-3 Primary Antibodies »

Product Specifications

Immunogen

KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # U26943

Specificity

Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody

Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) antibody by Western Blot.

Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Caspase-3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC).

Caspase‑3 in Jurkat Human Cell Line.

Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Caspase-3 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P).

Caspase‑3 in Human Colon Cancer Tissue.

Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Detection of Cleaved Caspase-3 antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry.

Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Caspase-3 by Western Blot

Detection of Mouse Caspase-3 by Western Blot

Epidermis-specific deletion of Rac1 increases UV-light-induced keratinocyte apoptosis in vivo. (a) H/E staining of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Black arrows indicate sunburn cells. (b) Graph shows the percentage number of sunburn cells at 12 h with (red bars) or without (blue bars) UV-irradiation in Rac1 fl/fl (n=4) and Rac1-EKO (n=5) mice. The percentage of sunburn cells within the epidermis after UV-irradiation in Rac1 fl/fl mice was 3.4%, whereas in Rac1-EKO mice it was 8.6% of total epidermal keratinocytes. Non-irradiated samples showed <1% sunburn cells in both the genotypes. (c) Immunostainings against cleaved caspase-3 (green) of UV-irradiated skin of Rac1 fl/fl and Rac1-EKO mice at 12 h after UV-irradiation. Nuclei are stained in blue. Scale bar=100 μm. (d) Western blot analysis of cleaved caspase-3 from epidermal lysates of untreated (no UV) and UV-light treated (UV) Rac1 fl/fl and Rac1-EKO mice. Non-irradiated controls showed no bands for cleaved capsase-3, whereas samples of irradiated epidermis showed cleaved caspase-3-specific bands at ~25 kDa and 23 kDa. Numbers on the left denote molecular weights in kDa. (e) Graph shows densitometry analysis of western blot in (d). Error bars show S.D. Asterisks show P-value<0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Caspase-3 by Western Blot

Detection of Mouse Caspase-3 by Western Blot

Rac1 deficiency increases sensitivity towards UV-light-induced keratinocyte apoptosis in vitro. (a and b) Western blot analysis of cleaved caspase-3 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h (a) and at 12 h (b) with (UV) or without (no UV) UV-irradiation. (c) Densitometry analysis of cleaved caspase-3 at 6 h after UV-irradiation from Rac1 fl/fl and Rac1-EKO keratinocytes. (d) Quantification of CPDs by CPD ELISA carried out from genomic DNA isolated from Rac1 fl/fl and Rac1-EKO mouse primary keratinocytes immediately after UV-irradiation. Error bars show S.D. Asterisks show P-value<0.01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Caspase-3 by Western Blot

Detection of Mouse Caspase-3 by Western Blot

Increase in UV-light-induced apoptosis in Rac1-deficient keratinocytes requires activation of caspase-8. (a) Western blot analysis of cleaved caspase-8 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (b) Densitometry analysis of fold change of cleaved caspase-8 in (a) normalized to GAPDH in Rac1 fl/fl and Rac1-EKO samples after UV-irradiation. (c) Western blot analysis of cleaved caspase-8 from CPDPL/Rac1-EKO epidermal lysates from mice kept in the dark or under the photoreactivtion lamp (PR). (d) Densitometric analysis of cleaved caspase-8 western blots in c. Error bars show S.D. (e) Western blot analysis of cleaved caspase-3 from wild-type (WT) and TNF receptor-1-deficient (TNFR-1 KO) cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (f) Western blot analysis of cleaved caspase-3 from TNFR-1 KO cultured keratinocytes incubated with DMSO or Rac1 inhibitor (EHT 1864) at 6 h with (UV) or without (no UV) UV-irradiation. GAPDH is used as a loading control. Numbers on the left denote molecular weights in kDa. * and *** represent P-value<0.05 and <0.001, respectively Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Caspase-3 by Western Blot

Detection of Rat Caspase-3 by Western Blot

Western blot analysis of high-mobility group box-1 (HMGB1), brain-derived neurotrophic factor (BDNF), synaptophysin, and cleaved caspase-3 in rat retinas. There is a significant increase in the expression of HMGB1 and cleaved caspase-3 and a significant decrease in the expression of BDNF and synaptophysin in the retinas of diabetic rats (D) compared with the nondiabetic control rats (N). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

BFSE decreases macrophage viability. THP-1 macrophage, or MDM cells, were exposure to air control or 1%, 2.5%, 5%, 10% BFSE or 10% CSE for 24 h. LDH from THP-1 macrophage or MDM cells was measured in supernatants after 24 h (n = 4) (A). THP-1 macrophage expression of Bcl-2 and PARP was assessed by western blot (B), band densitometry analysis of Bcl-2 (C) and PARP (D) was performed, data is a representation of three independent experiments, and was baselined to the air treated control sample, and normalized to beta -actin expression. Error bars represent 95% confidence intervals. Representative confocal images of active caspase-3 (E) and PAR (F), in THP-1 macrophage with quantitative MFI measurement of active caspase-3 (G) and PAR (H). (n = 4) *p < 0.05, **p < 0.01. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30194323), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Western Blot

Detection of Human Caspase-3 by Western Blot

Rac1 deficiency increases sensitivity towards UV-light-induced keratinocyte apoptosis in vitro. (a and b) Western blot analysis of cleaved caspase-3 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h (a) and at 12 h (b) with (UV) or without (no UV) UV-irradiation. (c) Densitometry analysis of cleaved caspase-3 at 6 h after UV-irradiation from Rac1 fl/fl and Rac1-EKO keratinocytes. (d) Quantification of CPDs by CPD ELISA carried out from genomic DNA isolated from Rac1 fl/fl and Rac1-EKO mouse primary keratinocytes immediately after UV-irradiation. Error bars show S.D. Asterisks show P-value<0.01 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Caspase-3 by Western Blot

Detection of Rat Caspase-3 by Western Blot

Western blot analysis of rat retinas. Intravitreal administration of high-mobility group box-1 (HMGB1) induced a significant upregulation of the expression of HMGB1 and cleaved caspase-3 and a significant downregulation of the expression of brain-derived neurotrophic factor (BDNF) and synaptophysin compared with intravitreal administration of phosphate buffer saline (PBS). Each experiment was repeated 2 to 3 times with fresh samples (n = 6). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23766563), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Apoptotic markers in PFII treated NHDFs.(A–C) [Ca2+]i fluorescence visualizations in cells as revealed by using an LSCM with fluorescent probe Fluo3/AM, scale bar - 100 µm. (D–F) Externalized phosphatidylserine by annexin V-fluorescein binding after PFII treatment, scale bar - 100 µm. Third (G–I) and fourth (J–L) panels show actin cytoskeletal organization and caspase-3 activation. Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Detection of Human Caspase-3 by Immunocytochemistry/Immunofluorescence

Caspase-3 activation and actin cytoskeletal organization in melanoma A375 cells cultured in the presence of PFII.Cells were grown on glass coverslips in the presence of 65, 130, 260 µM or 1 µM staurosporine (STS). (A–E) Active caspase-3 was visualized with anti-active caspase-3 antibody followed by a FITC-conjugated secondary antibody (green). (F–J) Actin was visualized using laser scanning confocal microscope (LSCM) after staining with Alexa Fluor 568 - conjugated phalloidin (red). Scale bar - 50 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0057991), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Caspase-3 by Western Blot

Detection of Mouse Caspase-3 by Western Blot

Increase in UV-light-induced apoptosis in Rac1-deficient keratinocytes requires activation of caspase-8. (a) Western blot analysis of cleaved caspase-8 from Rac1 fl/fl and Rac1-EKO cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (b) Densitometry analysis of fold change of cleaved caspase-8 in (a) normalized to GAPDH in Rac1 fl/fl and Rac1-EKO samples after UV-irradiation. (c) Western blot analysis of cleaved caspase-8 from CPDPL/Rac1-EKO epidermal lysates from mice kept in the dark or under the photoreactivtion lamp (PR). (d) Densitometric analysis of cleaved caspase-8 western blots in c. Error bars show S.D. (e) Western blot analysis of cleaved caspase-3 from wild-type (WT) and TNF receptor-1-deficient (TNFR-1 KO) cultured keratinocytes at 6 h with (UV) or without (no UV) UV-irradiation. (f) Western blot analysis of cleaved caspase-3 from TNFR-1 KO cultured keratinocytes incubated with DMSO or Rac1 inhibitor (EHT 1864) at 6 h with (UV) or without (no UV) UV-irradiation. GAPDH is used as a loading control. Numbers on the left denote molecular weights in kDa. * and *** represent P-value<0.05 and <0.001, respectively Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/28277539), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Western Blot

Detection of Mouse Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Western Blot

Gamma herpesvirus 68 ( gamma HV68) replicates in alveolar epithelial cells (AECs). (A) Wild-type mice were infected with 5 × 104 PFU gamma HV68 on day 0. On day 7 after infection, frozen sections were prepared, and stained with a rabbit polyclonal antisera against gamma HV68, or with non-immune rabbit sera as control. The goat anti-rabbit secondary was linked to alkaline phosphatase. Vivid replication of gamma HV68 is visible in alveolar lining cells (original magnification × 100). Sections shown are representative of four mice examined. (B) AECs were isolated from lungs of Balb/c or TLR-9-/- mice treated with bleomycin plus gamma HV68 on day 21, and were cultured on fibronectin-coated slides (TiterTek). Sections were stained with antibodies (M30 Cytodeath), and the number of positive cells per high power field (HPF; ×400) were calculated (n = 30 HPF per genotype). (C) AECs were isolated from Balb/c or TLR-9-/- mice and cells were infected in vitro with 0.01 or 0.001 PFU gamma HV68 for 48 hours. Cell lysates were then analyzed for cleaved caspase 3 by western blotting. Data are from one experiment, representative of two. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21810214), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Immunohistochemistry

Detection of Human Human/Mouse Cleaved Caspase-3 (Asp175) Antibody by Immunohistochemistry

Assessment of the skin structure and apoptosis upon treatment with wound gels. Representative images and zoom-ins of the boxed areas of haematoxylin and eosin (H&E)-stained tape-stripped (TS) sections from biopsies (age 38 years) either left untreated or treated with indicated wound gels using a light microscope. The presence of caspase-3 expressing cells was evaluated using a caspase-3 (CASP3) antibody and visualisation with a secondary Alexa Fluor™ 546 AB (red). Nuclei were counterstained with DAPI (blue). Scale bar = 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/36261541), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin

Immunohistochemistry

0.3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon cancer tissue

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: Jurkat human acute T cell leukemia cell line treated with Staurosporine was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol

Western Blot

0.5 µg/mL
Sample: Staurosporine-treated Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line

Reviewed Applications

Read 13 reviews rated 4.5 using MAB835 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Alternate Names

Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA

Entrez Gene IDs

836 (Human); 12367 (Mouse)

Gene Symbol

CASP3

UniProt

U26943

Additional Caspase-3 Products

Product Documents for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody

For research use only

Related Research Areas

Cysteine Proteases and Regulators

Citations for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody

Customer Reviews for Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody (13)

4.5 out of 5
13 Customer Ratings
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Showing  1 - 5 of 13 reviews Showing All
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  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult lung
    Species: Mouse
    Verified Customer | Posted 03/23/2025
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 09/23/2023
    In the immunoblot, our goal is to see the expressing differences within different treatments, and we clearly saw the bands. Although there were some non-specific bands, but they did not affect the discrimination between groups.
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 09/22/2022
    I used it to block this protein.
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Kidney tissue
    Species: Mouse
    Verified Customer | Posted 08/04/2021
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 02/16/2020
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/17/2018
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Liver tissue
    Species: Mouse
    Verified Customer | Posted 09/27/2017
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Byoung San Moon
    Application: Immunohistochemistry
    Sample Tested: Embryonic brain
    Species: Mouse
    Verified Customer | Posted 09/07/2017
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: IPS2 induced pluripotent stem cells derived cells
    Species: Human
    Verified Customer | Posted 02/08/2017
    Human/Mouse Cleaved Caspase‑3 (Asp175) Antibody MAB835
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22160308
    Species: Human
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23483962
    Species: Human
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23536519
    Species: Human
    Verified Customer | Posted 02/25/2015
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 23637762
    Species: Mouse
    Verified Customer | Posted 02/25/2015

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