Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

Catalog # Availability Size / Price Qty
MAB835
MAB835-SP
Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot.
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Product Details
Citations (62)
FAQs
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Reviews (7)

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
Source
Monoclonal Rabbit IgG Clone # 269518
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # U26943
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunohistochemistry
0.3-25 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) antibody by Western Blot. View Larger

Detection of Human and Mouse Cleaved Caspase‑3 (Asp175) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 µM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase-3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry Caspase-3 antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

Caspase‑3 in Jurkat Human Cell Line. Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry Caspase-3 antibody in Human Colon Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

Caspase‑3 in Human Colon Cancer Tissue. Caspase-3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase-3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Cleaved Caspase-3 antibody in Jurkat Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Cleaved Caspase‑3 in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 µM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase-3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-3

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Entrez Gene IDs
836 (Human); 12367 (Mouse)
Alternate Names
Apopain; apoptosis-related cysteine protease; CASP3; CASP-3; caspase 3, apoptosis-related cysteine peptidase; Caspase3; Caspase-3; CPP32; CPP-32; CPP32B; CPP32SREBP cleavage activity 1; Cysteine protease CPP32; EC 3.4.22; EC 3.4.22.56; LICE-1; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; YAMA

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Citations for Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

62 Citations: Showing 1 - 10
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  1. CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines
    Authors: Y Kaibori, D Nagakubo
    Cancer Gene Therapy, 2022;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Cellular recovery after prolonged warm ischaemia of the whole body
    Authors: D Andrijevic, Z Vrselja, T Lysyy, S Zhang, M Skarica, A Spajic, D Dellal, SL Thorn, RB Duckrow, S Ma, PQ Duy, AU Isiktas, D Liang, M Li, SK Kim, SG Daniele, K Banu, S Perincheri, MC Menon, A Huttner, KN Sheth, KT Gobeske, GT Tietjen, HP Zaveri, SR Latham, AJ Sinusas, N Sestan
    Nature, 2022;608(7922):405-412.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. Insights into the Pharmacokinetics and In Vitro Cell-Based Studies of the Imidazoline I2 Receptor Ligand B06
    Authors: A Bagán, JA Morales-Ga, C Griñán-Fer, C Díaz, J Pérez Del, MC Ramos, F Vicente, B Pérez, J Brea, MI Loza, M Pallàs, C Escolano
    International Journal of Molecular Sciences, 2022;23(10):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. NF1 mutation drives neuronal�activity-dependent initiation of optic glioma
    Authors: Y Pan, JD Hysinger, T Barron, NF Schindler, O Cobb, X Guo, B Yalç?n, C Anastasaki, SB Mulinyawe, A Ponnuswami, S Scheaffer, Y Ma, KC Chang, X Xia, JA Toonen, JJ Lennon, EM Gibson, JR Huguenard, LM Liau, JL Goldberg, M Monje, DH Gutmann
    Nature, 2021;594(7862):277-282.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  5. Ziyuglycoside II inhibits the growth of digestive system cancer cells through multiple mechanisms
    Authors: Y Zhong, XY Li, F Zhou, YJ Cai, R Sun, RP Liu
    Chinese journal of natural medicines, 2021;19(5):351-363.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Measles virus exits human airway epithelia within dislodged metabolically active infectious centers
    Authors: CE Hippee, BK Singh, AL Thurman, AL Cooney, AA Pezzulo, R Cattaneo, PL Sinn
    PloS Pathogens, 2021;17(8):e1009458.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Facilitative lysosomal transport of bile acids alleviates ER stress in mouse hematopoietic precursors
    Authors: AK Persaud, S Nair, MF Rahman, R Raj, B Weadick, D Nayak, C McElroy, M Shanmugam, S Knoblaugh, X Cheng, R Govindaraj
    Nature Communications, 2021;12(1):1248.
    Species: Mouse
    Sample Types:
    Applications: ICC/IF
  8. Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
    Authors: AT Kwok, NS Mohamed, JF Plate, RR Yammani, S Rosas, TA Bateman, E Livingston, JE Moore, BA Kerr, J Lee, CM Furdui, L Tan, ML Bouxsein, VL Ferguson, LS Stodieck, DC Zawieja, MD Delp, XW Mao, JS Willey
    Scientific Reports, 2021;11(1):10469.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. CD8+ tissue-resident memory T cells promote liver fibrosis resolution by inducing apoptosis of hepatic stellate cells
    Authors: Y Koda, T Teratani, PS Chu, Y Hagihara, Y Mikami, Y Harada, H Tsujikawa, K Miyamoto, T Suzuki, N Taniki, T Sujino, M Sakamoto, T Kanai, N Nakamoto
    Nature Communications, 2021;12(1):4474.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Genome-wide analysis of copy number alterations led to the characterisation of PDCD10 as oncogene in ovarian cancer
    Authors: C De Marco, P Zoppoli, N Rinaldo, S Morganella, M Morello, V Zuccalà, MV Carriero, D Malanga, R Chirillo, P Bruni, C Malzoni, D Di Vizio, R Venturella, F Zullo, A Rizzuto, M Ceccarelli, G Ciliberto, G Viglietto
    Translational Oncology, 2021;14(3):101013.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. KDM5B promotes immune evasion by recruiting SETDB1 to silence retroelements
    Authors: SM Zhang, WL Cai, X Liu, D Thakral, J Luo, LH Chan, MK McGeary, E Song, KRM Blenman, G Micevic, S Jessel, Y Zhang, M Yin, CJ Booth, LB Jilaveanu, W Damsky, M Sznol, HM Kluger, A Iwasaki, MW Bosenberg, Q Yan
    Nature, 2021;598(7882):682-687.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Calebin A Potentiates the Effect of 5-FU and TNF-&beta (Lymphotoxin &alpha) against Human Colorectal Cancer Cells: Potential Role of NF-&kappaB
    Authors: C Buhrmann, AB Kunnumakka, B Popper, M Majeed, BB Aggarwal, M Shakibaei
    Int J Mol Sci, 2020;21(7):.
    Species: Human
    Sample Types: Whole Cell
    Applications: IF
  13. MicroRNA miR-24-3p reduces DNA damage responses, apoptosis, and susceptibility to chronic obstructive pulmonary disease
    Authors: J Nouws, F Wan, E Finnemore, W Roque, SJ Kim, IS Bazan, CX Li, CM Sköld, Q Dai, X Yan, M Chioccioli, V Neumeister, CJ Britto, J Sweasy, RS Bindra, ÅM Wheelock, JL Gomez, N Kaminski, PJ Lee, M Sauler
    JCI Insight, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Transcription factor 21 expression in injured podocytes of glomerular diseases
    Authors: J Usui, M Yaguchi, S Yamazaki, M Takahashi-, T Kawamura, S Kaneko, SV Seshan, P Ronco, K Yamagata
    Sci Rep, 2020;10(1):11516.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  15. The interruption of atypical PKC signaling and Temozolomide combination therapy against glioblastoma
    Authors: A Dey, SMA Islam, R Patel, M Acevedo-Du
    Cell Signal, 2020;77(0):109819.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  16. Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition
    Authors: LB Jones, S Kumar, CR Bell, VA Peoples, BJ Crenshaw, MT Coats, JA Scoffield, GC Rowe, B Sims, QL Matthews
    Pathogens, 2019;8(4):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Dot Blot
  17. The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination
    Authors: H Zahednasab, M Firouzi, S Kaboudania, Z Mojallal-T, S Karampour, H Keyvani
    Cytokine, 2019;113(0):417-426.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  18. Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity
    Authors: S Giraud, C Steichen, P Couturier, S Tillet, V Mallet, R Coudroy, JM Goujon, P Hannaert, T Hauet
    Biomed Res Int, 2019;2019(0):8572138.
    Species: Porcine
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Piwi-Interacting RNA1037 Enhances Chemoresistance and Motility in Human Oral Squamous Cell Carcinoma Cells
    Authors: G Li, X Wang, C Li, S Hu, Z Niu, Q Sun, M Sun
    Onco Targets Ther, 2019;12(0):10615-10627.
    Species: Xenograft
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. Tissue-regenerative potential of the secretome of gamma-irradiated peripheral blood mononuclear cells is mediated via TNFRSF1B-induced necroptosis
    Authors: E Simader, L Beer, M Laggner, V Vorstandle, A Gugerell, M Erb, P Kalinina, D Copic, D Moser, A Spittler, E Tschachler, H Jan Ankers, M Mildner
    Cell Death Dis, 2019;10(10):729.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Elaeagnus glabra f. oxyphylla Attenuates Scopolamine-Induced Learning and Memory Impairments in Mice by Improving Cholinergic Transmission via Activation of CREB/NGF Signaling
    Authors: E Sohn, HS Lim, YJ Kim, BY Kim, JH Kim, SJ Jeong
    Nutrients, 2019;11(6):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  22. The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway
    Authors: P Shen, J Chen, M Pan
    Mol Med Rep, 2018;18(3):3332-3340.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  23. Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function
    Authors: R Hamon, HB Tran, E Roscioli, M Ween, H Jersmann, S Hodge
    Sci Rep, 2018;8(1):13424.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  24. Effect of the S-nitrosoglutathione reductase inhibitor N6022 on bronchial hyperreactivity in asthma
    Authors: LG Que, Z Yang, NL Lugogo, RK Katial, SA Shoemaker, JM Troha, DM Rodman, RM Tighe, M Kraft
    Immun Inflamm Dis, 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  25. Trabecular meshwork morphogenesis: A comparative analysis of wildtype and anterior segment dysgenesis mouse models
    Authors: RL Rausch, RT Libby, AE Kiernan
    Exp. Eye Res., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis
    Authors: BS Moon, J Bai, M Cai, C Liu, J Shi, W Lu
    Nat Commun, 2018;9(1):401.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  27. Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo
    Authors: A Shaalan, G Carpenter, G Proctor
    Mol. Immunol., 2018;94(0):190-199.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway
    Authors: ZP You, MJ Yu, YL Zhang, K Shi
    Mol Med Rep, 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  29. Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis
    Authors: KR Greenow, M Zverev, S May, H Kendrick, GT Williams, T Phesse, L Parry
    Oncotarget, 2018;9(92):36430-36443.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. The effect of the cholinergic anti-inflammatory pathway on collagen-induced arthritis involves the modulation of dendritic cell differentiation
    Authors: D Liu, T Li, H Luo, X Zuo, S Liu, S Wu
    Arthritis Res. Ther., 2018;20(1):263.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  31. Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis
    Authors: T Rahim, P Becquart, ME Baeva, J Quandt
    J Neuroinflammation, 2018;15(1):270.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  32. The protective effects of Resveratrol against radiation-induced intestinal injury
    Authors: H Zhang, H Yan, X Zhou, H Wang, Y Yang, J Zhang, H Wang
    BMC Complement Altern Med, 2017;17(1):410.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  33. WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response
    Authors: S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra
    Cell Death Dis, 2017;8(7):e2955.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  34. Resveratrol fuels HER2 and ER?-positive breast cancer behaving as proteasome inhibitor
    Authors: C Andreani, C Bartolacci, K Wijnant, R Crinelli, M Bianchi, M Magnani, A Hysi, M Iezzi, A Amici, C Marchini
    Aging (Albany NY), 2017;9(2):508-523.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice
    Authors: X Wang, GX Xue, WC Liu, H Shu, M Wang, Y Sun, X Liu, YE Sun, CF Liu, J Liu, W Liu, X Jin
    Aging Cell, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  36. Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist
    Authors: K Sato, R Shirai, M Hontani, R Shinooka, A Hasegawa, T Kichise, T Yamashita, H Yoshizawa, R Watanabe, TA Matsuyama, H Ishibashi-, S Koba, Y Kobayashi, T Hirano, T Watanabe
    J Am Heart Assoc, 2017;6(4):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  37. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
    Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
    Sci Rep, 2017;7(1):8225.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Loss of Tmem30a leads to photoreceptor degeneration
    Authors: L Zhang, Y Yang, S Li, S Zhang, X Zhu, Z Tai, M Yang, Y Liu, X Guo, B Chen, Z Jiang, F Lu, X Zhu
    Sci Rep, 2017;7(1):9296.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways
    Authors: N Prevete, F Liotti, A Illiano, A Amoresano, P Pucci, A de Paulis, RM Melillo
    Oncoimmunology, 2017;6(4):e1293213.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  40. Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever
    PLoS Pathog, 2016;12(5):e1005656.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  41. Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells
    Authors: Lucian Beer
    J. Radiat. Res, 2016;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  42. H-Ras and K-Ras oncoproteins induce different tumor spectra when driven by the same regulatory sequences
    Cancer Res., 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  43. Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin
    Authors: Elaine Fuchs
    Cell, 2016;167(5):1323-1338.e14.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  44. Antitumor cytotoxicity induced by bone-marrow-derived antigen-presenting cells is facilitated by the tumor suppressor protein p53 via regulation of IL-12
    Authors: Tania L Slatter
    Oncoimmunology, 2016;5(3):e1112941.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  45. Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
    Authors: Himmelein S, Lindemann A, Sinicina I, Strupp M, Brandt T, Hufner K
    J Virol, 2015;89(10):5747-50.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  46. The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis.
    Authors: Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A
    Oncogene, 2015;34(29):3826-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  47. The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
    Authors: Diefenbacher M, Popov N, Blake S, Schulein-Volk C, Nye E, Spencer-Dene B, Jaenicke L, Eilers M, Behrens A
    J Clin Invest, 2014;124(8):3407-18.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  49. Edaravone protect against retinal damage in streptozotocin-induced diabetic mice.
    Authors: Yuan, Dongqing, Xu, Yidan, Hang, Hui, Liu, Xiaoyi, Chen, Xi, Xie, Ping, Yuan, Songtao, Zhang, Weiwei, Lin, Xiaojun, Liu, Qinghuai
    PLoS ONE, 2014;9(6):e99219.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  50. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation.
    Authors: Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I
    Int J Cancer, 2014;134(10):2489-503.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  51. Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever.
    Authors: Oestereich L, Rieger T, Neumann M, Bernreuther C, Lehmann M, Krasemann S, Wurr S, Emmerich P, de Lamballerie X, Olschlager S, Gunther S
    PLoS Negl Trop Dis, 2014;8(5):e2804.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics.
    Authors: Hahn W, Kuzmicic J, Burrill J, Donoghue M, Foncea R, Jensen M, Lavandero S, Arriaga E, Bernlohr D
    Am J Physiol Endocrinol Metab, 2014;306(9):E1033-45.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  53. Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane.
    Authors: Janek T, Krasowska A, Radwanska A, Lukaszewicz M
    PLoS ONE, 2013;8(3):e57991.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  54. The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer.
    Authors: Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I
    Cell Death Dis, 2013;4(0):e627.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  55. The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure.
    Authors: Downs I, Liu J, Aw TY, Adegboyega PA, Ajuebor MN
    PLoS ONE, 2012;7(6):e38051.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  56. FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis.
    Authors: Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F
    Cancer Res, 2012;72(13):3424-36.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  57. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010;15(4):463-73.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  58. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  59. In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression.
    Authors: Chen RY, Chen HX, Lin JX
    J. Exp. Clin. Cancer Res., 2010;29(0):13.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  60. Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury.
    Authors: Terayama R, Bando Y, Murakami K, Kato K, Kishibe M, Yoshida S
    Neuroscience, 2007;148(1):175-87.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  61. Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B.
    Authors: Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM
    J. Cell. Physiol., 2007;210(2):417-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  62. Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells.
    Authors: Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW
    J. Cell. Sci., 2006;119(0):4247-56.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse Cleaved Caspase-3 (Asp175) Antibody

Average Rating: 4.7 (Based on 7 Reviews)

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Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 09/22/2022
Application: B/N Sample Tested: HEK293 human embryonic kidney cell line Species: Human

I used it to block this protein.


Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 08/04/2021
Application: IHC Sample Tested: Kidney tissue Species: Mouse

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 02/16/2020
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 01/17/2018
Application: WB Sample Tested: MDA-MB-231 human breast cancer cell line Species: Human

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 09/27/2017
Application: Flow Sample Tested: Liver tissue Species: Mouse

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Byoung San Moon on 09/07/2017
Application: IHC Sample Tested: Embryonic brain Species: Mouse

Human/Mouse Cleaved Caspase-3 (Asp175) Antibody
By Anonymous on 02/08/2017
Application: ICC/IF Sample Tested: IPS2 induced pluripotent stem cells derived cells Species: Human