Human/Mouse DACH2 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: DACH2

DACH2 (Dachshund 2) is a 65 kDa (predicted) member of the DACH family of transcription factors. It is expressed in the dermomyotome and Mullerian duct, and promotes Mullerian duct formation and myogenin gene suppression. Human DACH2 is 599 amino acids (aa) in length and contains a DD1 domain in the N-terminus (aa 66‑162) that mediates DNA binding, and a C-terminal, coiled-coil DD2 domain (aa 452‑543) that interacts with EYA proteins. Multiple splice variants of DACH2 are known. There is an alternate start site at Met220 that may be accompanied by both a deletion of aa 259‑311 and a three aa substitution for aa 585‑599. In addition, there is a deletion of aa 163‑175 that may be accompanied by an Ala substitution for aa 584‑599. Finally, there is a 41 aa substitution for aa 1‑175. Over aa 419‑583, human DACH2 is 93% aa identical to mouse DACH2.

Product Datasheets