Home / DGK-iota / Human/Mouse DGK-iota Alexa Fluor® 647-conjugated Antibody

Human/Mouse DGK-iota Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB6435R Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB6435R-100UG

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Human/Mouse DGK-iota Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha,
-beta, -δ, -epsilon, -eta, -gamma, -kappa, -theta, or -zeta is observed.
Source
Monoclonal Mouse IgG2a Clone # 648538
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human DGK‑ι
Ser925-Val1065 (predicted)
Accession # O75912
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DGK-iota

Diacylglycerol kinase iota (DGK-iota ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-iota also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa 178-232 and aa 251-310), a catalytic domain (aa 372-507), and two ankyrin repeats (aa 958-990 and aa 997-1026). Over aa 925-1065, human DGK-iota shares 94% and 96% aa sequence identity with mouse and rat DGK-iota, respectively.

Long Name
Diacylglycerol Kinase, iota 64kDa
Entrez Gene IDs
9162 (Human); 320127 (Mouse); 688705 (Rat)
Alternate Names
DAG kinase iota; DGKI; DGKiota; DGK-iota; diacylglycerol kinase iota; diacylglycerol kinase, iota; Diglyceride kinase iota; EC 2.7.1.107

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