Human/Mouse Ghrelin Alexa Fluor® 594-conjugated Antibody

Catalog #: FAB8200T Datasheet / COA / SDS

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FAB8200T-100UG

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Human/Mouse Ghrelin Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse and human Ghrelin/Obestatin prepropeptide in ELISA. Detects human Ghrelin peptides (acylated and de-acylated) in ELISA.

Source

Monoclonal Rat IgG2a Clone # 883622

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Obestatin/Ghrelin prepropeptide
Gly24-Lys117
Accession # Q9EQX0

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Ghrelin

GHRL (Ghrelin/Obestatin Prepropeptide) is a 117 aa precursor peptide that is processed into two chains, Ghrelin (aa24-51) and Obestatin (aa76-98). The 18 kDa prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases. Mature Ghrelin is modified with an N-octanoyl group on serine 3 which is required for receptor binding. Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa. Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs. Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Entrez Gene IDs

51738 (Human); 58991 (Mouse); 59301 (Rat)

Alternate Names

appetite-regulating hormone; Ghrelin; ghrelin/obestatin preprohormone; ghrelin/obestatin prepropeptide; GHRL; Growth hormone secretagogue; Growth hormone-releasing peptide; Motilin-related Peptide; MTLRPgrowth hormone secretagogue receptor ligand; obestatin; Protein M46