Human/Mouse Heparan Sulfate 3‑O‑Sulfotransferase 4/HS3ST4 Alexa Fluor™ Plus 555‑conjugated Antibody

R&D Systems | Catalog # AF6085AFP555

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Polyclonal Sheep IgG
View all Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Primary Antibodies »

Product Specifications

Specificity

Detects human and mouse Heparan Sulfate 3-O-Sulfotransferase4/HS3ST4 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4

Heparan Sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of Heparan Sulfate that is predominantly found in mast cells. Both heparin and Heparan Sulfate contain disaccharide repeats of uronic acid and N‑acetylglucosamine and are modified by the same sulfotransferases (1, 2). The uronic acid residues can be sulfated at the 2-O position by Heparan Sulfate 2-O sulfotransferase (HS2ST). The N‑acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N‑deacetylase/N‑sulfotransferases (NDSTs), Heparan Sulfate 3-O sulfotransferases (HS3STs) and Heparan Sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome (3, 4). HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events (5). HS3ST4 can generate tetrasulfated Heparan Sulfate disaccharide, the most highly sulfated sugar found in biological samples (6, 7), and may have a role in assisting HSV-1 entry and spread (8). HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs (3, 5). The enzyme activity was assayed using an SDS-PAGE based method (9).

References

  1. Bernfield, M. et al. (1999) Annu. Rev. Biochem. 68:729.
  2. Esko, J.D. and Selleck, S.B. (2002) Annu. Rev. Biochem. 71:435.
  3. Shworak, N.W. et al. (1999) J. Biol. Chem. 274:5170.
  4. Xu, D. et al. (2005) Biochem. J. 386:451.
  5. Lawrence, R. et al. (2007) Matrix Biol. 26:442.
  6. Mochizuki, H. et al. (2003) J. Biol. Chem. 278:26780.
  7. Wu, Z.L. et al. (2004) J. Biol. Chem. 279:1861.
  8. Tiwari,V. et al. (2005) Biochem. Biophys. Res. Commun. 338:930.
  9. Wu, Z.L. et al. (2010) BMC Biotechnol. 10:11.

Alternate Names

3-OST-4

Entrez Gene IDs

9951 (Human)

Gene Symbol

HS3ST4

UniProt

Q9Y661

Additional Heparan Sulfate 3-O-Sulfotransferase 4/HS3ST4 Products

Product Documents

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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Associated Pathways

Articular Cartilage Extracellular Matrix
Articular Cartilage Extracellular Matrix Thumbnail