Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody Summary
Arg23-Ser219
Accession # P29459
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of IL‑12/IL‑35 p35 in PBMC's treated with 1µg/mL LPS for 24 hrs and monensin for 3 hrs vs untreated PBMC's by Flow Cytometry PBMC's treated with 1µg/mL LPS for 24 hrs and monensin for 3 hrs (A) vs untreated PBMC's (B) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 PerCP‑conjugated Monoclonal Antibody (Catalog # IC2191C) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
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Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody (Catalog # IC2191C) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-12/IL-35 p35
Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
- Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
- Collison, L.W. et al. (2007) Nature 450:566.
- Bardel, E. et al. (2008) J. Immunol. 181:6898.
- Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Product Datasheets
Citations for Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Combined dendritic cell and anti-TIGIT immunotherapy potentiates adaptive NK cells against HIV-1
Authors: Sánchez-Cerrillo, I;Agudo-Lera, M;Popova, O;Tsukalov, I;Calvet-Mirabent, M;de Los Santos, I;García-Fraile, L;Fuentes, P;Delgado-Arévalo, C;Alcain, J;Sánchez-Gaona, N;Grau-Expósito, J;Lázaro-Díez, M;Muñoz-Calleja, C;Alfranca, A;Genescà, M;Prado, JG;Vrbanac, V;Balazs, A;Buzón, MJ;Toribio, ML;Muñoz-Fernández, MA;Sánchez-Madrid, F;Martín-Gayo, E;
EMBO molecular medicine
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
IL-35-producing B cells in gastric cancer patients
Authors: Ke Wang, Jianming Liu, Jiansheng Li
Medicine (Baltimore)
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IL-35-producing B cells in gastric cancer patients
Authors: Ke Wang, Jianming Liu, Jiansheng Li
Medicine (Baltimore)
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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