Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2191C
Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody in Data
2 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
Monoclonal Mouse IgG1 Clone # 27537
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
PerCP (Excitation= nm, Emission= nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. View Larger

Detection of IL‑12/IL‑35 p35 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody (Catalog # IC2191C) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. View Larger

Detection of IL‑12/IL‑35 p35 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Monoclonal Antibody (Catalog # IC2191C) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Long Name
Interleukin 12 p35
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names
IL-12/IL-35 p35; IL-12A; IL35p35

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human/Mouse IL-12/IL-35 p35 PerCP-conjugated Antibody
By Anonymous on 05/30/2017
Application: Flow Sample Tested: Pancreas tissue Species: Mouse