Human/Mouse IL-12/IL-35 p35 Antibody
Human/Mouse IL-12/IL-35 p35 Antibody Summary
Arg23-Ser219
Accession # P29459
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12/IL-35 p35
Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
- Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
- Collison, L.W. et al. (2007) Nature 450:566.
- Bardel, E. et al. (2008) J. Immunol. 181:6898.
- Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Product Datasheets
Citations for Human/Mouse IL-12/IL-35 p35 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7
Authors: MM Halpert, V Konduri, D Liang, Y Chen, JB Wing, S Paust, JM Levitt, WK Decker
Stem Cells Dev., 2016;25(10):774-87.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot -
Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection
Authors: Li Wang
J Biol Chem, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region.
Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S
J Immunol, 2015;194(1):113-24.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
Nat Commun, 2015;6(0):6353.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry -
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay -
Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
J Immunol, 2014;192(2):804-16.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development -
Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
J Immunol, 2012;189(12):5590-601.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
Authors: Jindra C
Eur J Immunol, 2010;40(2):321-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation -
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
J. Allergy Clin. Immunol., 2009;123(1):239-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008;38(4):1012-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Authors: Nagarajan S, Selvaraj P
Vaccine, 2005;24(13):2264-74.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
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