Human/Mouse IL-12/IL-35 p35 Antibody

(10 citations)

Species Reactivity
Human, Mouse
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
Monoclonal Mouse IgG₁ Clone # 27537
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended
Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL-12 (Catalog # 219-IL)
CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells or mouse splenocytes treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3⁺ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References:
1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
2. Collison, L.W. et al. (2007) Nature 450:566.
3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Long Name:
Interleukin 12 p35
Entrez Gene IDs:
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names:
IL-12/IL-35 p35; IL-12A; IL35p35

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Species	Applications	Sample Type
Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection Authors: Li Wang J Biol Chem, 2016;0(0):. Species: Human Sample Type: Cell Lysates Application: WB Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1. Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R Proc Natl Acad Sci U S A, 2015;112(9):E957-65. Species: Mouse Sample Type: Whole Tissue Application: Bioassay Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region. Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S J Immunol, 2015;194(1):113-24. Species: Human Sample Type: Whole Cells Application: Neut Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo. Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M Nat Commun, 2015;6(0):6353. Species: Mouse Sample Type: Whole Cells Application: Flow Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27. Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D J Immunol, 2014;192(2):804-16. Species: Mouse Sample Type: Cell Culture Supernates Application: ELISA Development Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade. Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D J Immunol, 2012;189(12):5590-601. Species: Human Sample Type: whole cells Application: Neut Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC. Authors: Jindra C Eur J Immunol, 2010;40(2):321-9. Species: Human Sample Type: Cell Culture Supernates Application: IP Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma. Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A J. Allergy Clin. Immunol., 2009;123(1):239-48. Species: Human Sample Type: Whole Cells Application: Flow Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells. Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S Eur. J. Immunol., 2008;38(4):1012-23. Species: Human Sample Type: Whole Cells Application: Flow Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination. Authors: Nagarajan S, Selvaraj P Vaccine, 2005;24(13):2264-74. Species: Human Sample Type: Cell Lysates Application: WB

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