Human/Mouse IL-12/IL-35 p35 Antibody

Catalog # Availability Size / Price Qty
MAB1570-SP
MAB1570-100
MAB1570-500
Product Details
Citations (18)
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Supplemental Products
Reviews (1)

Human/Mouse IL-12/IL-35 p35 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Source
Monoclonal Mouse IgG1 Clone # 27537
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human IL-12 (Catalog # 219-IL)
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Human peripheral blood mononuclear cells or mouse splenocytes treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References
  1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
  2. Collison, L.W. et al. (2007) Nature 450:566.
  3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
  4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
Long Name
Interleukin 12 p35
Entrez Gene IDs
3592 (Human); 16159 (Mouse); 84405 (Rat)
Alternate Names
CLMF;IL12A;IL-12A;IL-12p35;Ll12a;NFSK;NKSF1;P35; IL-12/IL-35 p35; IL-12A; IL35p35

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Citations for Human/Mouse IL-12/IL-35 p35 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Interleukin-35 expression is associated with colon cancer progression
    Authors: Jian Zhang, Tao Mao, Shuyun Wang, Dongsheng Wang, Zhaojian Niu, Zhenqing Sun et al.
    Oncotarget
  2. Engineering Rapalog-Inducible Genetic Switches Based on Split-T7 Polymerase to Regulate Oncolytic Virus-Driven Production of Tumour-Localized IL-12 for Anti-Cancer Immunotherapy
    Authors: Nikolas T. Martin, Mathieu J. F. Crupi, Zaid Taha, Joanna Poutou, Jack T. Whelan, Sydney Vallati et al.
    Pharmaceuticals (Basel)
  3. Operational immune tolerance towards transplanted allogeneic pancreatic islets in mice and a non-human primate
    Authors: Midhat H. Abdulreda, Dora M. Berman, Alexander Shishido, Christopher Martin, Maged Hossameldin, Ashley Tschiggfrie et al.
    Diabetologia
  4. Effects of phased joint intervention on IL-35 and IL-17 expression levels in patients with portal hypertension
    Authors: YUGANG WANG, JINBIN DONG, WENYING MENG, JIALI MA, NA WANG, JUE WEI et al.
    International Journal of Molecular Medicine
  5. Interleukin-35 Prevents the Elevation of the M1/M2 Ratio of Macrophages in Experimental Type 1 Diabetes
    Authors: Z Luo, C Soläng, R Larsson, K Singh
    International Journal of Molecular Sciences, 2022-07-19;23(14):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Epigenetic DNA Methylation of EBI3 Modulates Human Interleukin-35 Formation via NFkB Signaling: A Promising Therapeutic Option in Ulcerative Colitis
    Authors: A Wetzel, B Scholtka, F Schumacher, H Rawel, B Geisendörf, B Kleuser
    International Journal of Molecular Sciences, 2021-05-19;22(10):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  7. EBV-induced gene 3 augments IL-23R&alpha protein expression through a chaperone calnexin
    Authors: I Mizoguchi, M Ohashi, H Hasegawa, Y Chiba, N Orii, S Inoue, C Kawana, M Xu, K Sudo, K Fujita, M Kuroda, SI Hashimoto, K Matsushima, T Yoshimoto
    J. Clin. Invest., 2020-11-02;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection
    Authors: Li Wang
    J Biol Chem, 2016-06-15;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7
    Authors: MM Halpert, V Konduri, D Liang, Y Chen, JB Wing, S Paust, JM Levitt, WK Decker
    Stem Cells Dev., 2016-05-02;25(10):774-87.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  10. Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
    Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
    Nat Commun, 2015-02-19;6(0):6353.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
    Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
    Proc Natl Acad Sci U S A, 2015-02-17;112(9):E957-65.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  12. Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region.
    Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S
    J Immunol, 2014-12-01;194(1):113-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  13. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
    Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
    J Immunol, 2013-12-11;192(2):804-16.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  14. Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
    Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
    J Immunol, 2012-11-14;189(12):5590-601.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
    Authors: Jindra C
    Eur J Immunol, 2010-02-01;40(2):321-9.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Flow Cytometry, Immunoprecipitation
  16. Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
    Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
    J. Allergy Clin. Immunol., 2008-12-05;123(1):239-48.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
    Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
    Eur. J. Immunol., 2008-04-01;38(4):1012-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
    Authors: Nagarajan S, Selvaraj P
    Vaccine, 2005-12-07;24(13):2264-74.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Human/Mouse IL-12/IL-35 p35 Antibody
By Anonymous on 08/06/2018
Application: WB Sample Tested: Cell Culture Supernates Species: Mouse