Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Monoclonal Mouse IgG1 Clone # 27537
Protein A or G purified from ascites
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35 Arg23-Ser219 Accession # P29459
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Human peripheral blood mononuclear cells or mouse splenocytes treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-12/IL-35 p35
12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha
and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is
comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+
regulatory T cells and may function as an inhibitory cytokine to suppress T
cell proliferation (2). Human FoxP3+ Tregs do not constitutively
express IL-35 (3), but expression may be induced by activated dendritic cells
Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
R&D Systems personnel manually curate a database that contains references using R&D Systems products.
The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.