Human/Mouse IL-12/IL-35 p35 Antibody

Name: Human/Mouse IL-12/IL-35 p35 Antibody
Brand: R&D Systems
Rating: 4 (1 reviews)

MAB1570 11 Citations 1 Review Datasheet / COA / SDS

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MAB1570-SP
MAB1570-100
MAB1570-500

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Citations (11)

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Reviews (1)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse IL-12/IL-35 p35 Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

Source

Monoclonal Mouse IgG₁ Clone # 27537

Purification

Protein A or G purified from ascites

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human IL-12 (Catalog # 219-IL)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

Human peripheral blood mononuclear cells or mouse splenocytes treated with LPS, fixed with paraformaldehyde, and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-12/IL-35 p35

Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3⁺ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3⁺ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).

References

Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
Collison, L.W. et al. (2007) Nature 450:566.
Bardel, E. et al. (2008) J. Immunol. 181:6898.
Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.

Long Name

Interleukin 12 p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Alternate Names

IL-12/IL-35 p35; IL-12A; IL35p35

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Mouse IL-12/IL-35 p35 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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Dendritic Cell-Secreted Cytotoxic T-Lymphocyte-Associated Protein-4 Regulates the T-cell Response by Downmodulating Bystander Surface B7
Authors: MM Halpert, V Konduri, D Liang, Y Chen, JB Wing, S Paust, JM Levitt, WK Decker
Stem Cells Dev., 2016;25(10):774-87.
Species: Human
Sample Types: Cell Culture Supernates
Applications: WB
Gene expression and antiviral activity of interleukin-35 in response to influenza A virus infection
Authors: Li Wang
J Biol Chem, 2016;0(0):.
Species: Human
Sample Types: Cell Lysates
Applications: WB
Ex vivo-expanded but not in vitro-induced human regulatory T cells are candidates for cell therapy in autoimmune diseases thanks to stable demethylation of the FOXP3 regulatory T cell-specific demethylated region.
Authors: Rossetti M, Spreafico R, Saidin S, Chua C, Moshref M, Leong J, Tan Y, Thumboo J, van Loosdregt J, Albani S
J Immunol, 2015;194(1):113-24.
Species: Human
Sample Types: Whole Cells
Applications: Neut
Deltex1 antagonizes HIF-1alpha and sustains the stability of regulatory T cells in vivo.
Authors: Hsiao H, Hsu T, Liu W, Hsieh W, Chou T, Wu Y, Jiang S, Lai M
Nat Commun, 2015;6(0):6353.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow
Dendritic cell SIRT1-HIF1alpha axis programs the differentiation of CD4+ T cells through IL-12 and TGF-beta1.
Authors: Liu G, Bi Y, Xue L, Zhang Y, Yang H, Chen X, Lu Y, Zhang Z, Liu H, Wang X, Wang R, Chu Y, Yang R
Proc Natl Acad Sci U S A, 2015;112(9):E957-65.
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay
Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27.
Authors: Kochetkova I, Thornburg T, Callis G, Holderness K, Maddaloni M, Pascual D
J Immunol, 2014;192(2):804-16.
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: ELISA Development
Human prostate tumor antigen-specific CD8+ regulatory T cells are inhibited by CTLA-4 or IL-35 blockade.
Authors: Olson B, Jankowska-Gan E, Becker J, Vignali D, Burlingham W, McNeel D
J Immunol, 2012;189(12):5590-601.
Species: Human
Sample Types: Whole Cells
Applications: Neut
Human rhinoviruses induce IL-35-producing Treg via induction of B7-H1 (CD274) and sialoadhesin (CD169) on DC.
Authors: Jindra C
Eur J Immunol, 2010;40(2):321-9.
Species: Human
Sample Types: Cell Culture Supernates
Applications: IP
Der p 1 suppresses indoleamine 2, 3-dioxygenase in dendritic cells from house dust mite-sensitive patients with asthma.
Authors: Maneechotesuwan K, Wamanuttajinda V, Kasetsinsombat K, Huabprasert S, Yaikwawong M, Barnes PJ, Wongkajornsilp A
J. Allergy Clin. Immunol., 2009;123(1):239-48.
Species: Human
Sample Types: Whole Cells
Applications: Flow
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Authors: Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
Eur. J. Immunol., 2008;38(4):1012-23.
Species: Human
Sample Types: Whole Cells
Applications: Flow

Human tumor membrane vesicles modified to express glycolipid-anchored IL-12 by protein transfer induce T cell proliferation in vitro: a potential approach for local delivery of cytokines during vaccination.
Authors: Nagarajan S, Selvaraj P
Vaccine, 2005;24(13):2264-74.
Species: Human
Sample Types: Cell Lysates
Applications: WB