Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 27537
View all IL-12/IL-35 p35 Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑12/IL-35 p35
Arg23-Ser219
Accession # P29459

Specificity

Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Antibody

Detection of IL‑12/IL‑35 p35 in PBMC's treated with 1µg/mL LPS for 24 hrs and monensin for 3 hrs vs untreated PBMC's by Flow Cytometry

PBMC's treated with 1µg/mL LPS for 24 hrs and monensin for 3 hrs (A) vs untreated PBMC's (B) were stained with Mouse Anti-Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Monoclonal Antibody (Catalog # IC2191A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of IL-12/IL-35 p35 antibody in Human PBMCs or Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑12/IL‑35 p35 in Human PBMCs or Mouse Splenocytes by Flow Cytometry.

(A) Human peripheral blood mononuclear cell (PBMCs) or (B) mouse splenocytes either untreated (light orange filled histogram) or treated with LPS (dark orange filled histogram) were stained with Mouse Anti-Human/Mouse IL-12/IL-35 p35 APC-conjugated Monoclonal Antibody (Catalog # IC2191A) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cell (PBMCs) and mouse splenocytes treated with LPS were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IL-12/IL-35 p35

p35 is a 34-36 kDa monomeric member of the IL-6 superfamily of molecules. It is 219 amino acids in length, and possibly contains an N-terminal 22 aa signal sequence. In any event, it does not appear to be individually secreted, and its release from cells is commonly assumed to be as part of a disulfide-linked heterodimer linked to either p40 (forming IL-12) or EBI3 (forming IL-35). Multiple cell types are known to express p35, and include keratinocytes, CD138+ plasma cells, naïve B cells, monocyte-derived dendritic cells, macrophages, CD86lo B7-H1hi CD206+ B7-DC+ tolerogenic dendritic cells, neutrophils, activated monocytes and macrophages, trophoblasts, testicular endothelial cells, and T cells, including CD4+ and CD8+ T helper cells, and CD4+ CD25+ Tregs. The presence of the subunit does not necessarily indicate function, as the p35:p40 heterodimer is associated with promoting inflammatory IFN-gamma production by NK cells, while the p35:EBI3 heterodimer is anti-inflammatory through its inhibition of Th17 cell development. Over aa 23-219, human p35 shares 83% and 57% aa sequence identity with pig and mouse p35, respectively.

Long Name

Interleukin 12 p35

Alternate Names

IL-12A, IL35p35

Entrez Gene IDs

3592 (Human); 16159 (Mouse); 84405 (Rat)

Gene Symbol

IL12A

UniProt

P29459

Additional IL-12/IL-35 p35 Products

Product Documents for Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse IL‑12/IL‑35 p35 APC‑conjugated Antibody

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