Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
Intracellular Staining by Flow Cytometry
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Preparation and Storage
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-12/IL-35 p35
12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha
and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is
comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+
regulatory T cells and may function as an inhibitory cytokine to suppress T
cell proliferation (2). Human FoxP3+ Tregs do not constitutively
express IL-35 (3), but expression may be induced by activated dendritic cells
Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.