Human/Mouse IL-12/IL-35 p35 APC-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human IL‑12/IL-35 p35 in direct ELISAs and Western blots. Detects the p35 subunit either as part of a p40/p35 heterodimer or as a free subunit after reduction of the heterodimer. This antibody does not recognize IL-12 p40 homodimers but shows strong cross-reactivity with the p35 subunits from porcine and mouse systems.
  • Source
    Monoclonal Mouse IgG1 Clone # 27537
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Stability & Storage
    Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
Background: IL-12/IL-35 p35
Interleukin 12 (IL-12) and interleukin 35 (IL-35) are heterodimeric cytokines composed of alpha and beta chains. IL-12 is composed of p35 and p40 subunits, while IL-35 is comprised of p35 paired with EBI-3 (1). In mice, IL-35 is produced by FoxP3+ regulatory T cells and may function as an inhibitory cytokine to suppress T cell proliferation (2). Human FoxP3+ Tregs do not constitutively express IL-35 (3), but expression may be induced by activated dendritic cells (4).
  • References:
    1. Collison, L.W. and D.A.A. Vignali (2008) Immunol. Rev. 226:248.
    2. Collison, L.W. et al. (2007) Nature 450:566.
    3. Bardel, E. et al. (2008) J. Immunol. 181:6898.
    4. Seyerl, M. et al.(2010) Eur. J. Immunol. 40:321.
  • Long Name:
    Interleukin 12 p35
  • Entrez Gene IDs:
    3592 (Human); 16159 (Mouse); 84405 (Rat)
  • Alternate Names:
    IL-12A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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Species
Sample Type
  1. Regulatory T cells that recognize a ubiquitous stress-inducible self-antigen are long-lived suppressors of autoimmune arthritis.
    Authors: van Herwijnen M, Wieten L, van der Zee R, van Kooten P, Wagenaar-Hilbers J, Hoek A, den Braber I, Anderton S, Singh M, Meiring H, van Els C, van Eden W, Broere F
    Proc Natl Acad Sci U S A, 2012;109(35):14134-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow Cytometry,
  2. Differential regulation of interleukin-12 (IL-12)/IL-23 by Tim-3 drives T(H)17 cell development during hepatitis C virus infection.
    Authors: Wang, Jia M, Shi, Lei, Ma, Cheng J, Ji, Xiao J, Ying, Ruo S, Wu, Xiao Y, Wang, Ke S, Li, Guangyu, Moorman, Jonathan, Yao, Zhi Q
    J Virol, 2013;87(8):4372-83.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow Cytometry,
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 4
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Staining Reagents
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Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 3
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 2
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007
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