Human/Mouse IL-5 Alexa Fluor® 700-conjugated Antibody

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R&D Systems Antibodies
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Human/Mouse IL-5 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse IL-5 in ELISAs. In sandwich immunoassays with BAM6051 as the detection antibody, no cross-reactivity with recombinant human (rh) IL-3, rhGM-CSF, or recombinant mouse (rm) IL-5 is observed. In sandwich ELISAs with BAM705 as the detection antibody, no cross-reactivity with rhIL-5, rmIL-3, or rmGM-CSF is observed.
Monoclonal Rat IgG1 Clone # TRFK5
Mouse T cell clone-derived partially purified recombinant mouse IL-5
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)


Recommended Concentration
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
Human peripheral blood monocytes treated with PMA and Calcium Ionomycin, fixed with paraformaldehyde and permeabilized with saponin

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-5

IL-5 is a disulfide-linked homodimeric cytokine that is secreted by T cells. IL-5 promotes the proliferation, differentation, and activation of eosinophils. It binds to a receptor complex consisting of one IL-5 specific alpha chain and one non-binding common beta chain that is shared with the receptors for GM-CSF and IL-3.

Long Name
Interleukin 5
Entrez Gene IDs
3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline)
Alternate Names
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I

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Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.


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Human/Mouse IL-5 Alexa Fluor® 700-conjugated Antibody
By Oliver Burton on 09/12/2017
Application: Flow Sample Tested: Mouse splenocytes Species: Mouse

Splenocytes from C57BL/6 and Foxp3-deficient Scurfy mice were stimulated for 4 hours with phorbol 12,13-dibutyrate (500ng/ml), ionomycin (750ng/ml) and brefeldin A (2ug/ml). Cells were stained with fixable viability dye, then fixed with 2% formalin, permeabilized, and stained overnight for cytokines and gating markers (CD3, CD4).

Foxp3-deficient mice were chosen as a positive control because they are highly Th2-biased. IL-5 is not normally present in C57BL/6 CD4s in any great quantity. Another appropriate positive control would be ILC2 (e.g., gut lamina propria leukocytes).

Unstimulated cells that were otherwise treated identically served as a negative control.

Flow diagrams are shown gating on viable CD3+CD4+ T cells.