Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 261034
View all MSK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MSK2
Met1-Ser772
Accession # O75676

Specificity

Detects human and mouse MSK2 in Western blots. Does not detect recombinant human MSK1.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse MSK2 Antibody

Detection of Human/Mouse MSK2 antibody by Western Blot.

Detection of Human/Mouse MSK2 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 1 µg/mL Rat Anti-Human/Mouse MSK2 Monoclonal Antibody (Catalog # MAB2310) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional reference, recombinant human MSK1 and MSK2 (2 ng/lane) were included. A specific band for MSK2 was detected at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human/Mouse MSK2 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, DU145 human prostate carcinoma cell line, and TS1 mouse helper T cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: MSK2

Mitogen- and stress-activated protein kinase 2 (MSK2), also known as ribosomal protein S6 kinase B (RSKB) and RPS6KA4, belongs to the family of AGC kinases. Both MSK2 and the related MSK1 have two kinase domains connected by a regulatory linker region and are activated by the mitogen-activated protein kinases ERK1, ERK2, and p38. Nuclear MSK phosphorylates and activates a number of transcription factors, including ATF1 and CREB.

Long Name

Mitogen- and Stress-activated Protein Kinase 2

Alternate Names

RPS6KA4, RSKB

Entrez Gene IDs

8986 (Human); 56613 (Mouse)

Gene Symbol

RPS6KA4

UniProt

O75676

Additional MSK2 Products

Product Documents for Human/Mouse MSK2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse MSK2 Antibody

For research use only

Citations for Human/Mouse MSK2 Antibody

Customer Reviews for Human/Mouse MSK2 Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse MSK2 Antibody and earn rewards!

Have you used Human/Mouse MSK2 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

MAPK Signaling: Oxidative Stress Pathway MAPK Signaling: Oxidative Stress Pathway Thumbnail