Human/Mouse MYF-5 Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
IC4027G-100UG
Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry
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Human/Mouse MYF-5 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human MYF-5 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 593128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry View Larger

Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF‑5 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC4027G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MYF-5

MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Long Name
Myogenic Regulatory Factor 5
Entrez Gene IDs
4617 (Human); 17877 (Mouse); 299766 (Rat)
Alternate Names
bHLHc2; bHLHc2Class C basic helix-loop-helix protein 2; MYF5; MYF-5; myogenic factor 5

Product Datasheets

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