Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Transgenic Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 593128
View all MYF-5 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MYF-5
Met1-Leu255
Accession # P13349

Specificity

Detects human MYF-5 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse MYF‑5 Antibody

MYF-5 antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC).

MYF‑5 in C2C12 Mouse Cell Line.

MYF-5 was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human/Mouse MYF-5 Monoclonal Antibody (Catalog # MAB4027) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of MYF‑5 antibody in C2C12 Mouse Cell Line antibody by Flow Cytometry.

Detection of MYF‑5 in C2C12 Mouse Cell Line by Flow Cytometry.

C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse MYF-5 Monoclonal Antibody (Catalog # MAB4027, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human/Mouse MYF‑5 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed C2C12 mouse myoblast cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line fixed with paraformaldehyde and permeabilized with saponin

Reviewed Applications

Read 1 review rated 5 using MAB4027 in the following applications:

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MYF-5

MYF-5 (Myogenic Factor 5), also known as bHLHc2, is a 37-39 kDa transcriptional activator that belongs to the bHLH superfamily of myogenic regulatory factors. It is found in embryonic skeletal muscle progenitors and neurons, and is induced by SHH and Wnt1. Expression of MYF-5 protein prompts nonmuscle cells to adopt a muscle precursor phenotype. Notably, neurons appear to block MYF-5 accumulation, thus preserving their unique phenotype. MYF-5 forms heterodimers with multiple molecules. When complexed to I-mfa, MYF-5 is retained in the cytoplasm and is inactive; when complexed to E12, MYF-5 is transcriptionally active. Phosphorylation regulates MYF-5 stability. Human MYF-5 is 255 amino acids (aa) in length. It contains one myogenic basic region (aa 1-83) followed by an HLH motif (aa 96-135). Full-length human MYF-5 (aa 1-255) shares 89% aa sequence identity with mouse MYF-5.

Long Name

Myogenic Regulatory Factor 5

Alternate Names

bHLHc2, MYF5

Entrez Gene IDs

4617 (Human); 17877 (Mouse); 299766 (Rat)

Gene Symbol

MYF5

UniProt

P13349 (Human)

Additional MYF-5 Products

Product Documents for Human/Mouse MYF‑5 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MYF‑5 Antibody

For research use only

Citations for Human/Mouse MYF‑5 Antibody

Customer Reviews for Human/Mouse MYF‑5 Antibody (1)

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  • Human/Mouse MYF-5 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: C2C12 mouse myoblast cell line
    Species: Mouse
    Verified Customer | Posted 05/31/2022
    Human/Mouse MYF‑5 Antibody MAB4027

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Protocols

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