Human/Mouse Neurexin 3/NRXN3 Antibody

Catalog # Availability Size / Price Qty
AF5269
AF5269-SP
Human/Mouse Neurexin 3/NRXN3 Antibody in Western Blot
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Human/Mouse Neurexin 3/NRXN3 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Neurexin 3/NRXN3 in Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Neurexin 1 beta is observed and less than 5% cross-reactivity recombinant rat (rr) Neurexin 1 alpha and rrNeurexin 2 beta is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Neurexin 3/NRXN3
Ser35-Thr357
Accession # NP_620426
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
Immersion fixed paraffin-embedded sections of human brain (cerebellum and cortex)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human and Mouse Neurexin 3/NRXN3 by Western Blot. View Larger

Detection of Human and Mouse Neurexin 3/NRXN3 by Western Blot. Western blot shows lysates of mouse brain tissue and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse Neurexin 3/NRXN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5269) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Neurexin 3/NRXN3 at approximately 160-170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurexin 3/NRXN3

The alpha and beta forms of Neurexins 1‑3 are transmembrane neuronal glycoproteins which are transcribed from each of three NRXN genes that utilize alternate promoters. Like other Neurexins, the extracellular domain (ECD) of Neurexin 3 alpha contains six LNS domains interspersed with three EGF-like domains, while that of Neurexin 3 beta contains only the sixth LNS domain and no EGF-like domains (1‑3). Mature human Neurexin 3 beta is a 70 kDa glycosylated protein with a 528 amino acid (aa) ECD and a 56 aa cytoplasmic domain that contains a motif for binding PDZ scaffolding proteins (3‑5). Within comparable regions of the ECD, human Neurexin 3 beta shares 99% aa sequence identity with mouse and rat Neurexin 3 beta. It shares 65% aa sequence identity with comparable regions of the ECD of human Neurexin 1 beta and 2 beta. Alternative splicing of human Neurexin 3 beta generates multiple isoforms. There are potentially soluble and secreted variants and some which contain a fibronectin type III-like domain (4, 6). Neurexin 3 beta is widely expressed in the brain where it binds the postsynaptic Neuroligins 1, 2, and 3 (6‑9). Neurexin 3 beta may also be expressed in non-nervous tissues with a potentially cardiac-specific isoform (10). Human Neurexin 3 beta polymorphisms which affect the splicing pattern are associated with susceptibility to alcohol dependence (6). The Neurexin 3 beta genetic locus has been linked to opioid and nicotine addiction, and Neurexin 3 beta gene expression is up‑regulated after short term exposure of mice to cocaine (11‑13).

References
  1. Craig, A.M. and Y. Kang (2007) Curr. Opin. Neurobiol. 17:43.
  2. Dean, C. and T. Dresbach (2006) Trends Neurosci. 29:21.
  3. Lise, M.-F. and A. El-Husseini (2006) Cell. Mol. Life Sci. 63:1833.
  4. Rowen, L. et al. (2002) Genomics 79:587.
  5. Ushkaryov, Y. A. et al. (1994) J. Biol. Chem. 269:11987.
  6. Hishimoto, A. et al. (2007) Hum. Mol. Genet. 16:2880.
  7. Ullrich, B. et al. (1995) Neuron 14:497.
  8. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  9. Ichtchenko, K. et al. (1995) Cell 81:435.
  10. Occhi, G. et al. (2002) Biochem. Biophys. Res. Commun. 298:151.
  11. Lachman, H.M. et al. (2007) Hum. Mol. Genet. 16:1327.
  12. Bierut, L.J. et al. (2007) Hum. Mol. Genet. 16:24.
  13. Kelai, S. et al. (2008) Neuroreport 19:751.
Entrez Gene IDs
9369 (Human); 18191 (Mouse)
Alternate Names
C14orf60; chromosome 14 open reading frame 60; KIAA0743MGC176711; Neurexin 3; neurexin III; Neurexin III-alpha; Neurexin III-beta; neurexin-3-beta; NRXN3

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