Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 362616
View all PBEF/Visfatin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PBEF/Visfatin
Met1-His491
Accession # Q99KQ4

Specificity

Detects human and mouse PBEF/Visfatin in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human PBEF is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse PBEF/Visfatin Antibody

Detection of PBEF/Visfatin antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of PBEF/Visfatin in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Human/Mouse PBEF/ Visfatin Monoclonal Antibody (Catalog # MAB4044, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human/Mouse PBEF/Visfatin Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose‑like cell line fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human and mouse PBEF

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PBEF/Visfatin

PBEF, also called Nampt or visfatin, is a ubiquitous 52 kDa nicotinamide phosphoribosyltransferase. It is the rate-limiting component in the biosynthesis of NAD+, and functions in the cytoplasm to regulate energy metabolism during stress responses and immune activation. Although it lacks a signal sequence, PBEF appears to be secreted by visceral adipose tissue and functions as a noncompetitive insulin mimetic. Mouse PBEF shows 96% and > 99% aa identity with human and rat PBEF, respectively.

Long Name

Pre-B-Cell Colony-Enhancing Factor 1

Alternate Names

NAmPRTase, NAMPT, NMPRTase, PBEF1, Visfatin

Entrez Gene IDs

10135 (Human); 59027 (Mouse); 297508 (Rat)

Gene Symbol

NAMPT

UniProt

Q99KQ4

Additional PBEF/Visfatin Products

Product Documents for Human/Mouse PBEF/Visfatin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PBEF/Visfatin Antibody

For research use only

Citations for Human/Mouse PBEF/Visfatin Antibody

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FAQs

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Associated Pathways

Adipokines & Insulin Signaling Pathways Adipokines & Insulin Signaling Pathway Thumbnail