Human/Mouse Proprotein Convertase 2/PCSK2 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF6018AFP405

R&D Systems
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Sheep IgG
View all Proprotein Convertase 2/PCSK2 Primary Antibodies »

Product Specifications

Specificity

Detects human Proprotein Convertase 2/PCSK2 in direct ELISAs and, human and mouse Proprotein Convertase 2/PCSK2 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications

Application
Recommended Usage

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Proprotein Convertase 2/PCSK2

Proprotein Convertase 2 (PCSK2) is a subtilisin-like serine peptidase that processes proteins into biologically active products in the secretory pathway. Like other furin/kexin family members, PCSK2 cleaves its substrates primarily after sites of paired basic amino acid residues (1). Autoactivation of PCSK2 occurs in a post‑Golgi compartment of the secretory system (2). Interaction with the secretory protein 7B2 is required for proper activation of PCSK2 (3). The N-terminal domain of 7B2 stabilizes active PCSK2, and a C-terminal fragment of 7B2 can inhibit PCSK2 activity (4). rhPCSK2 was coexpressed with 7B2 to facilitate activation of the enzyme. PCSK2 is a major proteolytic processing enzyme of the regulated secretory pathway of the neuroendocrine system, where it generates a number of hormones and neuropeptides (5). Products of PCSK2 processing include enkephalins, insulin, somatostatin, dynorphin, and LHRH. Within the region used as an immunogen, human PCSK2 shares 97% aa identity with mouse PCSK2.

References

  1. Zhou, A. et al. (1999) J. Biol. Chem. 274:20745.
  2. Lamango, N.S. et al. (1999) Arch. Biochem. Biophys. 362:275.
  3. Zhu, X. and I. Lindberg (1995) J. Cell Biol. 129:1641.
  4. Lamango, N. et al. (1996) Arch. Biochem. Biophys. 330:238.
  5. Rouillé, Y. et al. (1995) Front. Neuroendocrinol. 16:322.

Long Name

Proprotein Convertase Subtilisin/Kexin Type 2

Alternate Names

NEC2, PC2, PCSK2, SPC2

Entrez Gene IDs

5126 (Human); 18549 (Mouse); 25121 (Rat)

Gene Symbol

PCSK2

UniProt

P16519 (Human)

Additional Proprotein Convertase 2/PCSK2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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