Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Alexa Fluor 488 (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 731502
Loading...

Product Specifications

Immunogen

E. coli-derived recombinant human Crystallin/CRYAB
Met1-Lys175
Accession # P02511

Specificity

Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: AlphaB Crystallin/CRYAB

CRY-alpha B (crystalline alpha -B chain/CRYAB; also HspB5) is a 22-23 kDa member of the HSP20 family of proteins.  It has widespread expression, and is found in lens epithelium where it noncovalently oligomerizes with CRYAA to generate a transparent 350-1000 kDa alpha -crystalline protein complex.  Human CRYAB is 175 amino acids (aa) in length. It contains an alpha -crystalline Hsp domain over aa 66-149.  Multiple post-translational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved.  It may also be phosphorylated at Ser45 and 59, be potentially O-GlcNAc modified at Thr158, 162 or 170, and acetylated at Lys92.  An alternate start site at Met68 may be accompanied by a 47 aa substitution for aa 109-175.  Full-length human CRYAB shares 54% aa sequence identity with CRYAA, and 98% aa identity with mouse CRYAB.  

Alternate Names

CRYA2, CRYAB, CTPP2, HSPB5

Entrez Gene IDs

1410 (Human); 12955 (Mouse); 25420 (Rat)

Gene Symbol

CRYAB

UniProt

Additional AlphaB Crystallin/CRYAB Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Customer Reviews

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat AlphaB Crystallin/CRYAB Alexa Fluor® 488‑conjugated Antibody and earn rewards!

Have you used Human/Mouse/Rat AlphaB Crystallin/CRYAB Alexa Fluor® 488‑conjugated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies