Detects human AlphaB Crystallin/CRYAB in direct ELISAs and human, mouse, and rat AlphaB Crystallin/CRYAB in Western blots. In direct ELISAs, no cross-reactivity with recombinant human AlphaA Crystallin/CRYAA is observed.
Monoclonal Mouse IgG2B Clone # 731502
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Crystallin/CRYAB Met1-Lys175 Accession # P02511
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human, Mouse, and Rat AlphaB Crystallin/CRYAB by Western Blot. Western blot shows lysates of rat heart tissue, mouse heart tissue, and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human AlphaB Crystallin/CRYAB and recombinant human AlphaA Crystallin/CRYAA (5 ng/lane) were included. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human AlphaB Crystallin/CRYAB by Simple WesternTM. Simple Western lane view shows lysates of human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for AlphaB Crystallin/CRYAB at approximately 28 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human/Mouse/Rat AlphaB Crystallin/CRYAB Monoclonal Antibody (Catalog # MAB4849). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: AlphaB Crystallin/CRYAB
CRY-alpha B (crystalline alpha -B chain/CRYAB; also HspB5) is a 22-23 kDa member of the HSP20 family of proteins. It has widespread expression, and is found in lens epithelium where it noncovalently oligomerizes with CRYAA to generate a transparent 350-1000 kDa alpha -crystalline protein complex. Human CRYAB is 175 amino acids (aa) in length. It contains an alpha -crystalline Hsp domain over aa 66-149. Multiple post-translational modifications may exist. The N-terminal Met and MetAspIleAlaIleHis sequence is occasionally cleaved. It may also be phosphorylated at Ser45 and 59, be potentially O-GlcNAc modified at Thr158, 162 or 170, and acetylated at Lys92. An alternate start site at Met68 may be accompanied by a 47 aa substitution for aa 109-175. Full-length human CRYAB shares 54% aa sequence identity with CRYAA, and 98% aa identity with mouse CRYAB.
Entrez Gene IDs:
1410 (Human); 12955 (Mouse); 25420 (Rat)
alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
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