CCK-A R in Rat Pancreas.
CCK-A R was detected in perfusion fixed frozen sections of rat pancreas using 5 µg/mL Human/Mouse/Rat CCK-A R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2680) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCK-A R
The physiologic actions of the cholecystokinin (CCK) family of peptide hormones are mediated through two classes of G protein-coupled receptors, CCK-A R and CCK-B R. CCK-A R is a 428 amino acid protein with 7 putative transmembrane domains. This GPCR is involved in gallbladder contraction, insulin secretion, and has also been described in specific regions of the central nervous system. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction.
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