Human/Mouse/Rat CCK-AR Antibody

R&D Systems | Catalog # AF2680

R&D Systems
Discontinued Product
AF2680 has been discontinued. View all CCK-AR products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all CCK-AR Primary Antibodies »

Product Specifications

Immunogen

Rat CCK-A R synthetic peptide
Lys256-Glu267
Accession # P30551

Specificity

Detects human, mouse, and rat CCK-A R.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat CCK-AR Antibody

CCK-A R antibody in Rat Pancreas by Immunohistochemistry (IHC-Fr).

CCK-A R in Rat Pancreas.

CCK-A R was detected in perfusion fixed frozen sections of rat pancreas using 5 µg/mL Human/Mouse/Rat CCK-A R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2680) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat CCK-AR Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse heart and kidney and rat pancreas

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCK-A R
Recombinant Mouse CCK-A R
Recombinant Rat CCK-A R

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCK-AR

The physiologic actions of the cholecystokinin (CCK) family of peptide hormones are mediated through two classes of G protein-coupled receptors, CCK-A R and CCK-B R. CCK-A R is a 428 amino acid protein with 7 putative transmembrane domains. This GPCR is involved in gallbladder contraction, insulin secretion, and has also been described in specific regions of the central nervous system. CCK-A R has been implicated in pancreatic tumorigenesis as well as the pathogenesis of eating disorders and drug addiction.

Long Name

Cholecystokinin Receptor, Type A

Alternate Names

CCK-A R, CCK1R, CCKAR

Entrez Gene IDs

886 (Human); 12425 (Mouse)

Gene Symbol

CCKAR

UniProt

P30551

Additional CCK-AR Products

Product Documents for Human/Mouse/Rat CCK-AR Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CCK-AR Antibody

For research use only

Citations for Human/Mouse/Rat CCK-AR Antibody

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Protocols

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