Human/Mouse/Rat CCR5 PE-conjugated Antibody

(7 citations)
(2 Reviews)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
  • Source
    Monoclonal Mouse IgG1 Clone # CTC5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with human CCR5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB1802P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins
Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL‑12 (Catalog # 419-ML) and 10 μg/mL Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE‑conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

  • References:
    1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
  • Entrez Gene IDs:
    1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
  • Alternate Names:
    C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Species
Applications
Sample Type
  1. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015;112(6):E556-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
    Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
    Blood, 2015;125(13):2087-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2010;184(2):1102-10.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008;22(8):2747-57.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
    Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
    J. Immunol., 2006;177(9):5811-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
    Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
    J. Immunol., 2002;169(8):4172-82.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 PE-conjugated Antibody

Ctrl IC002P 38  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 4 (Based on 2 reviews)

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We have 2 review tested in 1 application: Flow Cytometry.
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Images Ratings Applications Species Reviewed By Date Details
Flow Cytometry Human/Mouse/Rat CCR5 PE‑conjugated Antibody FAB1802P
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Very Good
 Flow Anonymous 10/26/2015
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Image Details
Flow Cytometry Human/Mouse/Rat CCR5 PE‑conjugated Antibody FAB1802P
Flow Cytometry: Human/Mouse/Rat CCR5 PE‑conjugated Antibody [FAB1802P]

Summary

ApplicationFlow Cytometry
Sample TestedCCR5-transfected MOLT4 cells

Other Experimental Details

Other Experimental DetailsHuman CCR5-transfected MOLT4 cells were stained with either a PE-conjugated isotype control or the anti-CCR5 antibody. Staining was performed at room temperature for 10 minutes using the dilution and buffers stated above.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS with 2.5% Calf Serum
Dilution: 1/10
Flow Cytometry Human/Mouse/Rat CCR5 PE‑conjugated Antibody FAB1802P
Enlarge
Very Good
 Flow Anonymous 10/26/2015
Show
Image Details
Flow Cytometry Human/Mouse/Rat CCR5 PE‑conjugated Antibody FAB1802P
Flow Cytometry: Human/Mouse/Rat CCR5 PE‑conjugated Antibody [FAB1802P]

Summary

ApplicationFlow Cytometry
Sample TestedSplenocytes

Other Experimental Details

Other Experimental DetailsNormal rat spenocytes were stained for 10 min in room temperatures followed by 3 washes.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10

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