Human/Mouse/Rat CCR5 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1802P-025
FAB1802P-100
Detection of CCR5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human/Mouse/Rat CCR5 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Source
Monoclonal Mouse IgG1 Clone # CTC5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR5 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References
  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Citations for Human/Mouse/Rat CCR5 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Effector Vgamma9Vdelta2 T cells dominate the human fetal gammadelta T-cell repertoire.
    Authors: Dimova T, Brouwer M, Gosselin F, Tassignon J, Leo O, Donner C, Marchant A, Vermijlen D
    Proc Natl Acad Sci U S A, 2015;112(6):E556-65.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. CXCR4 expression on pathogenic T cells facilitates their bone marrow infiltration in a mouse model of aplastic anemia.
    Authors: Arieta Kuksin C, Gonzalez-Perez G, Minter L
    Blood, 2015;125(13):2087-94.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CD56highCD16-CD62L- NK cells accumulate in allergic contact dermatitis and contribute to the expression of allergic responses.
    Authors: Carbone T, Nasorri F, Pennino D
    J. Immunol., 2010;184(2):1102-10.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.
    Authors: Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
    J. Immunol., 2010;184(6):2886-98.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Lactoferrin, a major defense protein of innate immunity, is a novel maturation factor for human dendritic cells.
    Authors: Spadaro M, Caorsi C, Ceruti P, Varadhachary A, Forni G, Pericle F, Giovarelli M
    FASEB J., 2008;22(8):2747-57.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Systemic and local characterization of regulatory T cells in a chronic fungal infection in humans.
    Authors: Cavassani KA, Campanelli AP, Moreira AP, Vancim JO, Vitali LH, Mamede RC, Martinez R, Silva JS
    J. Immunol., 2006;177(9):5811-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
    Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
    J. Immunol., 2002;169(8):4172-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Mouse/Rat CCR5 PE-conjugated Antibody

Average Rating: 4 (Based on 2 Reviews)

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Human/Mouse/Rat CCR5 PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Cytometry Sample Tested: CCR5-transfected MOLT4 cells

Human CCR5-transfected MOLT4 cells were stained with either a PE-conjugated isotype control or the anti-CCR5 antibody. Staining was performed at room temperature for 10 minutes using the dilution and buffers stated above.
Specificity: Reasonably specific
Sensitivity: Reasonably sensitive
Buffer: PBS with 2.5% Calf Serum
Dilution: 1/10


Human/Mouse/Rat CCR5 PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Cytometry Sample Tested: Splenocytes

Normal rat spenocytes were stained for 10 min in room temperatures followed by 3 washes.
Specificity: Reasonably specific
Sensitivity: Sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10