CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.
Human/Mouse/Rat CCR5 PE‑conjugated Antibody
R&D Systems | Catalog # FAB1802P
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)
Applications
Validated:
Flow Cytometry
Cited:
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # CTC5
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Product Specifications
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Specificity
Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat CCR5 PE‑conjugated Antibody
Detection of CCR5 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P) or (B) Mouse IgG1Phycoerythrin Isotype Control (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Polyclonal Antibody (Catalog # AB-404-NA) for 3 days to induce Th1 development were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of CCR5 in Rat Splenocytes by Flow Cytometry.
Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 PE-conjugated Monoclonal Antibody (Catalog # FAB1802P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human/Mouse/Rat CCR5 PE‑conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes
Reviewed Applications
Read 2 reviews rated 4 using FAB1802P in the following applications:
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5
References
- Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Alternate Names
CCR5, CD195
Gene Symbol
CCR5
Additional CCR5 Products
Product Documents for Human/Mouse/Rat CCR5 PE‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human/Mouse/Rat CCR5 PE‑conjugated Antibody
For research use only
Citations for Human/Mouse/Rat CCR5 PE‑conjugated Antibody
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Customer Reviews for Human/Mouse/Rat CCR5 PE‑conjugated Antibody (2)
4 out of 5
2 Customer Ratings
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Application: Flow CytometrySample Tested: CCR5-transfected MOLT4 cellsVerified Customer | Posted 10/26/2015Human CCR5-transfected MOLT4 cells were stained with either a PE-conjugated isotype control or the anti-CCR5 antibody. Staining was performed at room temperature for 10 minutes using the dilution and buffers stated above. <br />Specificity: Reasonably specific<br />Sensitivity: Reasonably sensitive<br />Buffer: PBS with 2.5% Calf Serum<br />Dilution: 1/10
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Application: Flow CytometrySample Tested: SplenocytesVerified Customer | Posted 10/26/2015Normal rat spenocytes were stained for 10 min in room temperatures followed by 3 washes. <br />Specificity: Reasonably specific<br />Sensitivity: Sensitive<br />Buffer: PBS + 2.5% FBS<br />Dilution: 1/10
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
