Human/Mouse/Rat CCR5 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1802A-025
FAB1802A-100
Detection of CCR5 in Human PBMCs by Flow Cytometry.
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Product Details
Citations (8)
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Human/Mouse/Rat CCR5 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Source
Monoclonal Mouse IgG1 Clone # CTC5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Flow Cytometry Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry. View Larger

Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Poly-clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR5 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References
  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Citations for Human/Mouse/Rat CCR5 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. CCR5 antagonist blocks metastasis of basal breast cancer cells.
    Authors: Velasco-Velazquez M, Jiao X, De la Fuente M, Pestell T, Ertel A, Lisanti M, Pestell R
    Cancer Res, 2102;72(15):3839-50.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation
    Authors: JM Laufer, MA Hauser, I Kindinger, V Purvanov, A Pauli, DF Legler
    Cell Rep, 2019;29(4):995-1009.e6.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. CCR5 governs DNA damage and breast cancer stem cell expansion
    Authors: X Jiao, MA Velasco-Ve, M Wang, Z Li, H Rui, AR Peck, JE Korkola, X Chen, S Xu, JB DuHadaway, S Guerrero-R, S Addya, D Sicoli, Z Mu, G Zhang, A Stucky, X Zhang, M Cristofani, A Fatatis, JW Gray, JF Zhong, GC Prendergas, RG Pestell
    Cancer Res., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CIB1 and CIB2 are HIV-1 helper factors involved in viral entry
    Sci Rep, 2016;6(0):30927.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. CCR5 receptor antagonists block metastasis to bone of v-Src oncogene-transformed metastatic prostate cancer cell lines.
    Authors: Sicoli D, Jiao X, Ju X, Velasco-Velazquez M, Ertel A, Addya S, Li Z, Ando S, Fatatis A, Paudyal B, Cristofanilli M, Thakur M, Lisanti M, Pestell R
    Cancer Res, 2015;74(23):7103-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  7. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010;24(4):491-502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

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