Human/Mouse/Rat CCR5 APC-conjugated Antibody

(6 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
  • Source
    Monoclonal Mouse IgG1 Clone # CTC5
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    CHO Chinese hamster ovary cell line transfected with human CCR5
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CCR5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3 epsilon Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG1 Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins
Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL‑12 (Catalog # 419‑ML) and 10 μg/mL Goat Anti-Mouse IL‑4 Poly­clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR5 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC‑conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

  • References:
    1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
  • Entrez Gene IDs:
    1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
  • Alternate Names:
    C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Species
Applications
Sample Type
  1. CCR5 antagonist blocks metastasis of basal breast cancer cells.
    Authors: Velasco-Velazquez M, Jiao X, De la Fuente M, Pestell T, Ertel A, Lisanti M, Pestell R
    Cancer Res, 2102;72(15):3839-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. CIB1 and CIB2 are HIV-1 helper factors involved in viral entry
    Sci Rep, 2016;6(0):30927.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. CCR5 receptor antagonists block metastasis to bone of v-Src oncogene-transformed metastatic prostate cancer cell lines.
    Authors: Sicoli D, Jiao X, Ju X, Velasco-Velazquez M, Ertel A, Addya S, Li Z, Ando S, Fatatis A, Paudyal B, Cristofanilli M, Thakur M, Lisanti M, Pestell R
    Cancer Res, 2015;74(23):7103-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. IL-27 in human secondary lymphoid organs attracts myeloid dendritic cells and impairs HLA class I-restricted antigen presentation.
    Authors: Morandi, Fabio, Di Carlo, Emma, Ferrone, Soldano, Petretto, Andrea, Pistoia, Vito, Airoldi, Irma
    J Immunol, 2014;192(6):2634-42.
  5. HIV-1 infection is characterized by profound depletion of CD161+ Th17 cells and gradual decline in regulatory T cells.
    Authors: Prendergast A, Prado JG, Kang YH, Chen F, Riddell LA, Luzzi G, Goulder P, Klenerman P
    AIDS, 2010;24(4):491-502.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells.
    Authors: Bayry J, Triebel F, Kaveri SV, Tough DF
    J. Immunol., 2007;178(7):4184-93.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG1 APC-conjugated Antibody

Ctrl IC002A 8  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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