Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # CTC5
View all CCR5 Primary Antibodies »

Product Specifications

Immunogen

CHO Chinese hamster ovary cell line transfected with human CCR5

Specificity

Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat CCR5 APC‑conjugated Antibody

Detection of CCR5 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of CCR5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG1Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR5 antibody in Mouse Splenocytes antibody by Flow Cytometry.

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Poly-clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR5 antibody in Rat Splenocytes antibody by Flow Cytometry.

Detection of CCR5 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat CCR5 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes

Reviewed Applications

Read 2 reviews rated 5 using FAB1802A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Spectra Viewer

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References

  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

Additional CCR5 Products

Product Documents for Human/Mouse/Rat CCR5 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CCR5 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse/Rat CCR5 APC‑conjugated Antibody

Customer Reviews for Human/Mouse/Rat CCR5 APC‑conjugated Antibody (2)

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  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID:22637726
    Species: Human
    Verified Customer | Posted 01/21/2015
  • Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: See PMID: 22637726
    Species: Human
    Verified Customer | Posted 01/21/2015

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