Human CCR5 Antibody

Catalog # Availability Size / Price Qty
MAB1802-100
MAB1802-SP
Detection of CCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (2)

Human CCR5 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.
Source
Monoclonal Mouse IgG1 Clone # CTC5
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
CHO Chinese hamster ovary cell line transfected with human CCR5
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
Lee, B. et al. (1999) J. Biol. Chem. 274:9617. This application was not tested by R&D Systems.
 
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of CCR5 antibody in Human Peripheral Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of CCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB1802) or (B) control antibody (Catalog # MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CD3 APC-conjugated monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References
  1. Lee, B. et al. (1999) J. Biol. Chem. 274:9617.
Entrez Gene IDs
1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)
Alternate Names
C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Citations for Human CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies
    Authors: M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic
    Journal of Translational Medicine, 2022;20(1):39.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Modeling of CCR5 Recognition by HIV-1 gp120: How the Viral Protein Exploits the Conformational Plasticity of the Coreceptor
    Authors: C Jacquemard, F Koensgen, P Colin, B Lagane, E Kellenberg
    Viruses, 2021;13(7):.
    Species: Human
    Sample Types: Membranes
    Applications: Flow Cytometry
  3. CCR5 structural plasticity shapes HIV-1 phenotypic properties
    Authors: P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane
    PLoS Pathog., 2018;14(12):e1007432.
    Species: Human
    Sample Types: Whole Cells
    Applications: Competitive Binding Assay
  4. A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody.
    Authors: Chain B, Arnold J, Akthar S, Brandt M, Davis D, Noursadeghi M, Lapp T, Ji C, Sankuratri S, Zhang Y, Govada L, Saridakis E, Chayen N
    PLoS ONE, 2015;10(6):e0128381.
    Species: Human
    Sample Types: Peptide
    Applications: ELISA Development
  5. Divergent effects of hypoxia on dendritic cell functions.
    Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
    Blood, 2008;112(9):3723-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
    Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
    J. Biol. Chem., 1999;274(14):9617-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. A maraviroc-resistant HIV-1 with narrow cross-resistance to other CCR5 antagonists depends on both N-terminal and extracellular loop domains of drug-bound CCR5.
    Authors: Tilton J, Wilen C, Didigu C, Sinha R, Harrison J, Agrawal-Gamse C, Henning E, Bushman F, Martin J, Deeks S, Doms R
    J Virol, 0;84(20):10863-76.

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Reviews for Human CCR5 Antibody

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Human CCR5 Antibody
By Anonymous on 11/29/2021
Application: Western Blot Sample Tested: Dendritic cells Species: Human

Human CCR5 Antibody
By Anonymous on 06/28/2017
Application: Western Blot Sample Tested: U937 human histiocytic lymphoma cell line Species: Human