Human CCR5 Antibody

Name: Human CCR5 Antibody
Brand: R&D Systems
SKU: MAB1802-SP
Rating: 5 (2 reviews)

MAB1802 7 Citations 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1802-100
MAB1802-SP

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Detection of CCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry.

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Citations (7)

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Reviews (2)

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.

Source

Monoclonal Mouse IgG₁ Clone # CTC5

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

CHO Chinese hamster ovary cell line transfected with human CCR5

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Lee, B. et al. (1999) J. Biol. Chem. 274:9617. This application was not tested by R&D Systems.

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of CCR5 in Human Peripheral Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB1802) or (B) control antibody (Catalog # MAB002), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B) and Mouse anti-Human CD3 APC-conjugated monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References

Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Alternate Names

C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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Identifying CCR5 coreceptor populations permissive for HIV-1 entry and productive infection: implications for in vivo studies
Authors: M Weichseldo, Y Tagaya, M Reitz, AL DeVico, OS Latinovic
Journal of Translational Medicine, 2022;20(1):39.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Modeling of CCR5 Recognition by HIV-1 gp120: How the Viral Protein Exploits the Conformational Plasticity of the Coreceptor
Authors: C Jacquemard, F Koensgen, P Colin, B Lagane, E Kellenberg
Viruses, 2021;13(7):.
Species: Human
Sample Types: Membranes
Applications: Flow Cytometry
CCR5 structural plasticity shapes HIV-1 phenotypic properties
Authors: P Colin, Z Zhou, I Staropoli, J Garcia-Per, R Gasser, M Armani-Tou, Y Benureau, N Gonzalez, J Jin, BJ Connell, S Raymond, P Delobel, J Izopet, H Lortat-Jac, J Alcami, F Arenzana-S, A Brelot, B Lagane
PLoS Pathog., 2018;14(12):e1007432.
Species: Human
Sample Types: Whole Cells
Applications: Competitive Binding Assay
A Linear Epitope in the N-Terminal Domain of CCR5 and Its Interaction with Antibody.
Authors: Chain B, Arnold J, Akthar S, Brandt M, Davis D, Noursadeghi M, Lapp T, Ji C, Sankuratri S, Zhang Y, Govada L, Saridakis E, Chayen N
PLoS ONE, 2015;10(6):e0128381.
Species: Human
Sample Types: Peptide
Applications: ELISA Development
Divergent effects of hypoxia on dendritic cell functions.
Authors: Mancino A, Schioppa T, Larghi P, Pasqualini F, Nebuloni M, Chen IH, Sozzani S, Austyn JM, Mantovani A, Sica A
Blood, 2008;112(9):3723-34.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
J. Biol. Chem., 1999;274(14):9617-26.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
A maraviroc-resistant HIV-1 with narrow cross-resistance to other CCR5 antagonists depends on both N-terminal and extracellular loop domains of drug-bound CCR5.
Authors: Tilton J, Wilen C, Didigu C, Sinha R, Harrison J, Agrawal-Gamse C, Henning E, Bushman F, Martin J, Deeks S, Doms R
J Virol, 0;84(20):10863-76.