Human/Mouse/Rat Contactin‑1 Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # AF7549AFP405

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Polyclonal Sheep IgG
View all Contactin-1 Primary Antibodies »

Product Specifications

Specificity

Detects human, mouse, and rat Contactin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Contactin-2 and rmContactin-4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for Human/Mouse/Rat Contactin‑1 Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Contactin-1

CNTN1 (Contactin-1; also neuronal cell surface protein F3/F11) is a 135-145 kDa GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules. It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners. In particular, it binds to astrocyte tenascin-C, Schwann cell NF/neurofascin-155, and oligodendrocyte produced tenascin-R and Notch in-trans, plus paranodin in-cis. Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation. CNTN1 also binds to chondroitin sulfate-E, an interaction that initiates neurite outgrowth. Mouse CNTN1 is synthesized as a 1020 amino acid (aa) preproprecursor (aa 1-1020). It contains a 20 aa signal sequence, followed by a 981 aa mature region (aa 21-1001), and a 19 aa propeptide. The mature region possesses six consecutive C2-type Ig‑like domains (aa 41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa 605-901) that regulate adhesiveness. It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK-1 antigen (3-sulfo-GluA-Gal-GlcNac). Over aa 20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively.

Alternate Names

CNTN1, Contactin1, F11, F3cam

Entrez Gene IDs

1272 (Human); 12805 (Mouse)

Gene Symbol

CNTN1

UniProt

AAH66864

Additional Contactin-1 Products

Product Documents for Human/Mouse/Rat Contactin‑1 Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Contactin‑1 Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways
Notch Signaling Pathway Thumbnail