Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Contactin-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Contactin-1
Asp21-Thr999
Accession # AAH66864

Specificity

Detects human, mouse, and rat Contactin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) Contactin-2 and rmContactin-4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Contactin‑1 Antibody

Detection of Human, Mouse, and Rat Contactin-1 antibody by Western Blot.

Detection of Human, Mouse, and Rat Contactin‑1 by Western Blot.

Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7549) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Contactin-1 at approximately 135 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Contactin-1 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

Contactin‑1 in Mouse Embryo.

Contactin-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti-Human/Mouse/Rat Contactin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7549) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to dorsal root ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat Contactin‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

1-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (13 d.p.c.)

Western Blot

0.2 µg/mL
Sample: Human brain (hypothalamus) tissue, mouse brain (cerebellum) tissue, and rat brain tissue

Reviewed Applications

Read 1 review rated 5 using AF7549 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Contactin-1

CNTN1 (Contactin-1; also neuronal cell surface protein F3/F11) is a 135-145 kDa GPI-linked glycoprotein member of the Contactin family, Ig superfamily of molecules. It is expressed on the surface of oligodendrocytes and neurons, principally on axons, and has multiple binding partners. In particular, it binds to astrocyte tenascin-C, Schwann cell NF/neurofascin-155, and oligodendrocyte produced tenascin-R and Notch in-trans, plus paranodin in-cis. Tenascin-R interactions inhibit neurite outgrowth, paranodin/NF155 interactions may contribute to Ranvier node development, and Notch ligation results in oligodendrocyte maturation. CNTN1 also binds to chondroitin sulfate-E, an interaction that initiates neurite outgrowth. Mouse CNTN1 is synthesized as a 1020 amino acid (aa) preproprecursor (aa 1-1020). It contains a 20 aa signal sequence, followed by a 981 aa mature region (aa 21-1001), and a 19 aa propeptide. The mature region possesses six consecutive C2-type Ig‑like domains (aa 41-603) that regulate neurite outgrowth, followed by three FN-type III domains (aa 605-901) that regulate adhesiveness. It is estimated that 15% of the native MW constitutes carbohydrate, part of which is represented by the HNK-1 antigen (3-sulfo-GluA-Gal-GlcNac). Over aa 20-999, mouse CNTN1 shares 96% and 99% aa sequence identity with human and rat CNTN1, respectively.

Alternate Names

CNTN1, Contactin1, F11, F3cam

Entrez Gene IDs

1272 (Human); 12805 (Mouse)

Gene Symbol

CNTN1

UniProt

AAH66864

Additional Contactin-1 Products

Product Documents for Human/Mouse/Rat Contactin‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Contactin‑1 Antibody

For research use only

Citations for Human/Mouse/Rat Contactin‑1 Antibody

Customer Reviews for Human/Mouse/Rat Contactin‑1 Antibody (1)

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  • Human/Mouse/Rat Contactin-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/05/2022
    Human/Mouse/Rat Contactin‑1 Antibody AF7549

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail