Human/Mouse/Rat DARPP-32 Alexa Fluor 405 Affinity Purified P

R&D Systems | Catalog # AF6259V

R&D Systems
Discontinued Product
AF6259V has been discontinued. View all DARPP-32 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human DARPP‑32
Arg51-Ala204
Accession # Q9UD71

Specificity

Detects human, mouse, and rat DARPP‑32 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse/Rat DARPP-32 Alexa Fluor 405 Affinity Purified P

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Alternate Names

DARPP32, PPP1R1B

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Gene Symbol

PPP1R1B

UniProt

Additional DARPP-32 Products

Product Documents for Human/Mouse/Rat DARPP-32 Alexa Fluor 405 Affinity Purified P

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat DARPP-32 Alexa Fluor 405 Affinity Purified P


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Related Research Areas

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Protocols

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FAQs

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