Human/Mouse/Rat DARPP-32 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF6259S Datasheet / COA / SDS

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AF6259S-100UG

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Human/Mouse/Rat DARPP-32 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat DARPP‑32 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DARPPâ€‘32
Arg51-Ala204
Accession # Q9UD71

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kDa protein that anomalously migrates at about 32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP‑32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name

Dopamine and cAMP-regulated Phosphoprotein

Entrez Gene IDs

84152 (Human); 19049 (Mouse); 360616 (Rat)

Alternate Names

DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)