Human/Mouse/Rat Erythropoietin Antibody

(1 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human, mouse, and rat Erythropoietin (Epo) in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse Thymopoietin (Tpo) is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin
    Ala27-Arg192
    Accession # P07321
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human Erythropoietin (Ultrapure) (Catalog # 286-EP)
    Recombinant Mouse Erythropoietin (Catalog # 959-ME)
    Recombinant Rat Erythropoietin (Catalog # 1306-RE)
  • Neutralization
    Measured by its ability to neutralize Erythropoietin-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 0.25-1.25 µg/mL in the presence of 10 ng/mL Recombinant Mouse Erythropoietin.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by Erythropoietin and Neutralization by Mouse Erythropoietin Antibody.

Recombinant Mouse Erythropoietin (Catalog #
959‑ME) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Erythropoietin (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat Erythropoietin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF959). The ND50 is typically 0.25-1.25 µg/mL.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Erythropoietin

Erythropoietin (Epo) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of Epo is required for biological activities in vivo (4). Mature mouse Epo shares 95% amino acid sequence identity with rat Epo and 73% - 82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6 - 8). Epo promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the Epo receptor (Epo R) (8, 9). Epo R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of Epo R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating Epo allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce Epo production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). Epo additionally plays a
tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

  • References:
    1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
    2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
    3. Wen, D. et al. (1993) Blood 82:1507.
    4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
    5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
    6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
    7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
    8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
    9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
    10. Acs, G. et al. (2001) Cancer Res. 61:3561.
    11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
    12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
    13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
  • Entrez Gene IDs:
    2056 (Human); 13856 (Mouse); 24335 (Rat)
  • Alternate Names:
    EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Suppression of hepcidin during anemia requires erythropoietic activity.
    Authors: Pak M, Lopez MA, Gabayan V, Ganz T, Rivera S
    Blood, 2006;108(12):3730-5.
    Species: Mouse
    Sample Type: In Vivo
    Application: In vivo
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 8
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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