Recombinant Mouse Erythropoietin/EPO Protein

Carrier Free

Catalog # Availability Size / Price Qty
959-ME-010/CF

With Carrier

Catalog # Availability Size / Price Qty
959-ME-010
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (16)
FAQs
Reviews (1)

Recombinant Mouse Erythropoietin/EPO Protein Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.4‑2 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Erythropoietin/EPO protein
Ala27-Arg192
Accession #
N-terminal Sequence
Analysis
Ala27
Predicted Molecular Mass
18.6 kDa
SDS-PAGE
36 kDa, reducing conditions

Product Datasheets

959-ME (with carrier)

959-ME/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

959-ME

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

959-ME/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N-glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of (EPO is required for biological activities in vivo (4). Mature mouse (EPO shares 95% amino acid sequence identity with rat (EPO and 73%-82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. Epo is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6-8). (EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the (EPO receptor (EPO R) (8, 9). (EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of (EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating (EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce (EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). (EPO additionally plays a tissue-protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References
  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.
Entrez Gene IDs
2056 (Human); 13856 (Mouse); 24335 (Rat)
Alternate Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2

Citations for Recombinant Mouse Erythropoietin/EPO Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4
    Authors: N Funk, M Munz, T Ott, K Brockmann, A Wenninger-, R Kühn, D Vogt-Weise, F Giesert, W Wurst, T Gasser, S Biskup
    Sci Rep, 2019;9(1):4515.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Establishment of an erythroid progenitor cell line capable of enucleation achieved with an inducible c-Myc vector
    Authors: S Mayers, PD Moço, T Maqbool, PN Silva, DM Kilkenny, J Audet
    BMC Biotechnol., 2019;19(1):21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Hto, Tritiated Amino Acid Exposure and External Exposure Induce Differential Effects on Hematopoiesis and Iron Metabolism
    Authors: JM Bertho, D Kereselidz, L Manens, C Culeux, V Magneron, J Surette, M Blimkie, L Bertrand, H Wyatt, M Souidi, I Dublineau, N Priest, JR Jourdain
    Sci Rep, 2019;9(1):19919.
    Species: Mouse
    Sample Types: Plasma
    Applications: Multiplex Standard
  4. FGF23 modulates the effects of erythropoietin on gene expression in renal epithelial cells
    Authors: M Yashiro, M Ohya, T Mima, Y Ueda, Y Nakashima, K Kawakami, Y Ishizawa, S Yamamoto, S Kobayashi, T Yano, Y Tanaka, K Okuda, T Sonou, T Shoshihara, Y Iwashita, Y Iwashita, K Tatsuta, R Matoba, S Negi, T Shigematsu
    Int J Nephrol Renovasc Dis, 2018;11(0):125-136.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Administration of nucleoside-modified mRNA encoding broadly neutralizing antibody protects humanized mice from HIV-1 challenge
    Authors: N Pardi, AJ Secreto, X Shan, F Debonera, J Glover, Y Yi, H Muramatsu, H Ni, BL Mui, YK Tam, F Shaheen, RG Collman, K Karikó, GA Danet-Desn, TD Madden, MJ Hope, D Weissman
    Nat Commun, 2017;8(0):14630.
    Species: Mouse
    Sample Types: Plasma
    Applications: ELISA Developmet
  6. Clostridium perfringens ?-toxin impairs erythropoiesis by inhibition of erythroid differentiation
    Authors: T Takagishi, M Takehara, S Seike, K Miyamoto, K Kobayashi, M Nagahama
    Sci Rep, 2017;7(1):5217.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Administration of erythropoietin prevents bone loss in osteonecrosis of the femoral head in mice
    Authors: T Xu, H Jin, Y Lao, P Wang, S Zhang, H Ruan, Q Mao, L Zhou, L Xiao, P Tong, C Wu
    Mol Med Rep, 2017;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  8. The Differential Effects of Erythropoietin Exposure to Oxidative Stress on Microglia and Astrocytes in vitro
    Authors: P Pathipati, DM Ferriero
    Dev. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. The mouse Char10 locus regulates severity of pyruvate kinase deficiency and susceptibility to malaria
    Authors: A Laroque, G Min-Oo, M Tam, P Ponka, MM Stevenson, P Gros
    PLoS ONE, 2017;12(5):e0177818.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  10. Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency
    Stem Cell Reports, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Active enhancers are delineated de novo during hematopoiesis, with limited lineage fidelity among specified primary blood cells.
    Authors: Luyten A, Zang C, Liu X, Shivdasani R
    Genes Dev, 2014;28(16):1827-39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Embryonic stem cell-derived haematopoietic progenitor cells down-regulate the CD3 xi chain on T cells, abrogating alloreactive T cells.
    Authors: Kim E, Miyake B, Aggarwal M, Voetlause R, Griffith M, Zavazava N
    Immunology, 2014;142(3):421-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate.
    Authors: Grover, Amit, Mancini, Elena, Moore, Susan, Mead, Adam J, Atkinson, Deborah, Rasmussen, Kasper D, O'Carroll, Donal, Jacobsen, Sten Eir, Nerlov, Claus
    J Exp Med, 2014;211(2):181-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Role of Gas6 in erythropoiesis and anemia in mice.
    Authors: Angelillo-Scherrer A, Burnier L, Lambrechts D, Fish RJ, Tjwa M, Plaisance S, Sugamele R, DeMol M, Martinez-Soria E, Maxwell PH, Lemke G, Goff SP, Matsushima GK, Earp HS, Chanson M, Collen D, Izui S, Schapira M, Conway EM, Carmeliet P
    J. Clin. Invest., 2008;118(2):583-96.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Differential requirements for hematopoietic commitment between human and rhesus embryonic stem cells.
    Authors: Rajesh&lt;/LastName&gt;&lt;ForeNam D&lt;/Initial, Rajesh D, Chinnasamy N, Mitalipov SM, Wolf DP, Slukvin I, Thomson JA, Shaaban AF
    Stem Cells, 2007;25(2):490-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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Recombinant Mouse Erythropoietin Protein
By Anonymous on 05/12/2016
Application: Immunoassay Standard