NMDAR2A Antibody

R&D Systems | Catalog # PPS012

R&D Systems
Discontinued Product
PPS012 has been discontinued. View all NMDAR2A products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all NMDAR2A Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor

Specificity

Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NMDAR2A Antibody

Detection of GRIN2A/NMDAR2A antibody by Western Blot.

Detection of GRIN2A/NMDAR2A by Western Blot.

Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the NMDA Receptor fusion protein (not shown).
Detection of GRIN2A/NMDAR2A by Western Blot

Detection of GRIN2A/NMDAR2A by Western Blot

Expression of neurodevelopmental proteins in the hippocampus and neocortex of adult KMO(−/−) mice. The panels illustrate the expression of several of the proteins examined in this study by Western blots (n = 4/group) using the hippocampus (HIPPO) and neocortex (CX). Data are shown for the optical density of blots relative to actin for (A) GluN2A (180 kDa), (B) PSD-95 (95 kDa), (C) DISC1 (70 kDa protein), (D) DISC1 (100 kDa protein), (E) Unc5H3 (130 kDa), (F) Shh (45 kDa), (G) doublecortin (DCX; 45 kDa) and (H) actin (42 kDa). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26365611), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for NMDAR2A Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:2000 dilution
Sample: Frozen sections; unpublished observations

Immunoprecipitation

3 μL per 200 μg lysate

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) lysate

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Formulation

Lyophilized from 5 mM ammonium bicarbonate

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

After reconstitution the antibody should be aliquoted and stored at ≤ -20° C. This product is stable at ≤- 20° C for at least 1 year.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NMDAR2A

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A

Alternate Names

EPND, FESD, GluN2A, glutamate receptor, ionotropic, N-methyl D-aspartate 2A, hNR2A, LKS, NMDA R, NR2A Subunit, NMDA receptor subtype 2A, NMDAR2A, N-methyl D-aspartate receptor subtype 2A, N-methyl-D-aspartate receptor subunit 2A, NR2A, subunit epsilon-1

Entrez Gene IDs

2903 (Human); 14811 (Mouse); 24409 (Rat)

Gene Symbol

GRIN2A

Additional NMDAR2A Products

Product Documents for NMDAR2A Antibody

Certificate of Analysis

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Product Specific Notices for NMDAR2A Antibody

For research use only

Citations for NMDAR2A Antibody

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Protocols

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