Human/Mouse/Rat GRIN2A/NMDAR2A Antibody

(3 citations)   
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Specific for the ~180 kDa NR2A subunit of the NMDA Receptor in Western blots of most brain regions. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Fusion protein from the C-terminus of the NR2A subunit of rat NMDA Receptor
  • Formulation
    Lyophilized from 5 mM ammonium bicarbonate
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1:1000 dilution
    See below
  • Immunohistochemistry
    1:1000 to 1:2000 dilution
    Frozen sections; unpublished observations
  • Immunoprecipitation
    3 μL per 200 μg lysate
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of GRIN2A/NMDAR2A by Western Blot. Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2A subunit of the NMDA Receptor. The labeling by the antibody was blocked by the N-terminal NR2A-subunit of the NMDA Receptor fusion protein (not shown).
Preparation and Storage
  • Reconstitution
    This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    After reconstitution the antibody should be aliquoted and stored at ≤ -20° C. This product is stable at ≤- 20° C for at least 1 year.
Background: GRIN2A/NMDAR2A
NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes, which code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2A form generates the highest conductance NMDAR. Rat NR2A is a 180 kDa, 1464 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 533 aa extracellular domain (ECD) and a 627 aa cytoplasmic region. The molecule is described as 4-TM. However, the second-TM segment is only partial (or reenterant), and this makes the C-terminus intracellular. The loop connecting TM segments 3 and 4, plus the N-terminal ECD constitutes the glutamate binding site. Rat NR2 is 99% and 95% aa identical to mouse and human NR2, respectively.
  • Long Name:
    Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2A
  • Entrez Gene IDs:
    2903 (Human); 14811 (Mouse); 24409 (Rat)
  • Alternate Names:
    EPND; FESD; GluN2A; GRIN2A; LKS; NMDA R, NR2A Subunit; NMDAR2A; NR2A
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Transmembrane protein 108 is required for glutamatergic transmission in dentate gyrus
    Authors: HF Jiao, XD Sun, R Bates, L Xiong, L Zhang, F Liu, L Li, HS Zhang, SQ Wang, MT Xiong, M Patel, AM Stranahan, WC Xiong, BM Li, L Mei
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(5):1177-1182.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
  2. Lrp4 in astrocytes modulates glutamatergic transmission
    Authors: Xiang-Dong Sun
    Nat Neurosci, 2016;0(0):.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  3. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012;5(0):22.
    Species: Rat
    Sample Type: Tissue Homogenates
    Application: WB
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